Team:KULeuven/Project/Output

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Pictogram output.png

Contents

Output

GFP.png

We have been searching for an easy accessible output system, that could provide us qualitative and quantitative information in a quick and easy way. The choice for GFP was easily made.

BioBricks

Project output.jpg

Components

A GFP with a C-terminal LVA tag for rapid degradation of the protein (Andersen et al, 1998) was chosen so that we can follow the output signal quickly in time. The Tet promoter ([http://partsregistry.org/Part:BBa_R0040 BBa_R0040]) behaves as described in the input section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 BBa_B0032] with a relative efficiency of 0,3.

Action

This is a very easy system. As the input results in inactivation of the tetR repressor, there is GFP production, proportional to the input signal.

References

Appl Environ Microbiol. 1998 Jun;64(6):2240-6.
New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria.
Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S.
PMID: 9603842



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