Team:KULeuven/30 August 2008

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Contents

Lab Work

Wet Lab

  • A glycerol stock was made of the new parts and of our own parts (K145151, K145001 and K145015).
  • We MiniPrepped the new parts and the two ligations that succeeded (C0040+B0015 and C0060+B0015).
  • The digestions with XbaI were started.
  • The ligations we made yesterday were electroporated today (still in electrocompetent Top10 cells).
  • A liquid culture was made of part R0053.

Dry Lab

Created the oligos for the B1002 and B1006 terminators [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=terminator link]

Modeling

Final updated model uploaded to the wiki (only MatLab)

Wiki

Remarks

I don't think the dam methylation is the problem with our ligations. This is the prefix: gaattcgcggccgcttctagag. The recognition site of XbaI is in bold. There is no overlap for dam methylation (gatc).

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