Team:Paris/Notebook/Freezer

From 2008.igem.org

Contents 1 Last number 2 PCR products 3 Plasmids: MiniPrep Products 4 Digestion Products 5 Strains (glycerol stock) Last number (check on 11 sept 2008, subject to change by unwritten on the wiki Last Ligation number used 185 Last PCR number used 154 Last Digestion number used 202 Last Miniprep number used 172 Last Strain number used 168 PCR products PCR products Number Name Oligo F Oligo R Template Length Comments Product Left ? [DNA]µg/mL Date PCR124 pFlgA O100 O101 - 261pb yes 0.5 August 7 PCR125 pFlgB O102 O103 - 261pb yes 0.5 August 7 PCR126 pFlhB O108 O109 - 260pb yes 0.5 August 7 PCR127 flhDC O132 O131 MG1655 no August 8 PCR128 OmpR* O138 O139 Strain OmpR* 700pb yes 150 August 8 PCR129 EnvZ* O126 O127 Strain EnvZ* 1400pb yes 100 August 8 PCR130 E0240 rbs+ O141 O140 MP143 900pb no August 11 PCR131 flhD rbs- O132 O133 MG1655 350pb no August 11 PCR132 pFlhD rbs- O130 O131 MG1655 600pb no August 11 PCR133 flhDC + prom O110 O131 MG1655 1300pb no August 11 PCR134 flhDC + prom O111 O131 MG1655 no August 11 PCR135 pfliL O124 O125 MG1655 yes August 11 PCR136 pFlhDC O110 O113 MG1655 yes August 11 PCR137 pFlhDC O 111 O 113 MG1655 yes August 11 PCR138 FlhDC (RBS -) O 132 O 131 MG 1655 yes August 15 PCR139 pflhDC (URI) O 110 O 113 PCR 130 yes August 15 PCR140 flhDC (RBS -) O 132 O 131 PCR 130 yes August 15 PCR141 Gene FlhDC + rbs nat O 134 O 131 MG1655 1000pb yes August 26 PCR142 pFliA + rbs nat O 145 O 144 MG1655 325pb yes August 26 PCR143 pFliA sans rbs O 145 O 146 MG1655 310pb yes August 26 PCR144 pFliA + Gene FliA O 145 O 151 MG1655 1100pb yes August 26 PCR145 PCRFliA1 O 148 O 152 MG1655 150pb yes August 26 PCR146 PCRFliA2 O 153 O 150 MG1655 670pb yes August 26 PCR 147 EnvZ* O 126 O 127 S 120 1412 bp yes 150 August 26 PCR 148 OmpR* O 138 O 139 S 119 789 bp yes 101 August 26 PCR 151 pFLiA without nat. rbs O 145 O 146 MG1655 310 bp yes 34 August 26 PCR 152 pFliA rbs nat. O 145 O 144 MG1655 325 bp yes 19 August 26 PCR 153 pFliA+Gene FliA non-mutated O 145 O 151 MG1655 1100 bp yes 39 August 26 PCR 154 Gene FliA mutated (EcoRI/PstI) O 148 O 150 MG1655 800 bp 154.1, 154.2, 154.3 yes 50 August 26 Plasmids: MiniPrep Products Plasmids: MiniPrep Products 2008 Number Name Plamsid Biobricks Description Products in use/comments Conc. ug/mL Date MP100 [http://partsregistry.org/Part:BBa_B0034 B0034 ] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS (Efficiency = 1) July 26 MP101.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Strongest Constitutive promoter July 26 333 July 22, July 23, July 26 MP101.2 finished MP101.4 200 MP102 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 pJ61002] Weak Constitutive promoter July 26 July 22, July 26 MP103 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] promoter pLas (lasr & PAI regulated) July 26 July 23, July 26 MP104.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressible promoter July 26 145 July 23, July 26 MP104.3 147 MP104.4 51 MP105.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) ->LasI 34 July 26 MP105.2 31 MP106.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) -> TetR 80 August 24 MP106.3 40 MP107 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] lasR activator with LVA Tag July 23 MP108 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] lasR activator July 26 July 22, July 23, July 26 MP109 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] YFP July 26 MP110 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] GFP July 26 July 23, July 26 MP111 [http://partsregistry.org/Part:BBa_E1010 E1010 ] [http://partsregistry.org/Part:pSB2K3 pSB2K3] mRFP July 26 July 23, July 26 MP112 OmpR M57I July 23 MP113 EnvZ I281S July 23 MP114.1 [http://partsregistry.org/Part:BBa_C0040 C0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressor 173 July 23 MP114.2 263 MP115 [http://partsregistry.org/Part:pSB1A3 pSB1A3] July 23 MP116 [http://partsregistry.org/Part:BBa_J23100 pJ23100] [http://partsregistry.org/Part:BBa_J61002 J61002] Strong constitutive promoter in J61002 July 26 MP117 [http://partsregistry.org/Part:BBa_J23107 pJ23107] [http://partsregistry.org/Part:BBa_J61002 J61002] Medium constitutive promoter in J61002 July 26 July 24, July 26 MP118.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Double terminator July 26 220 July 24, July 26 MP118.3 187 MP119.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] AraC pBAD July 26 42 July 24, July 26 MP119.2 26 MP119.3 39 MP120 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong RBS (Efficiency = 0,6) July 26 MP121 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] ECFP (RBS+LVA+Double Term) July 26 July 24, July 26 MP122 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] gfp tri-part; strong rbs July 26 MP123 [http://partsregistry.org/Part:J61002 J61002] August 5 MP124 L102 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong rbs - YFP D129 (BV) - D118 (BI) August 1 MP125 L103 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong rbs - mRFP D129 (BV) - D122 (BI) August 1 MP126 L104 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong rbs - lasR activator D129 (BV) - D114 (BI) August 1 MP127 L105 [http://partsregistry.org/Part:J61002 J61002] Strong promoter - ECFP D123 (BV) - D130 (BI) August 1 MP128 L106 [http://partsregistry.org/Part:J61002 J61002] Strong promoter - gfp Tripart D123 (BV) - D131 (BI) August 1 MP129 L107 [http://partsregistry.org/Part:J61002 J61002] Strong promoter - ECFP D103 (BV) - D130 (BI) August 1 MP130 L108 [http://partsregistry.org/Part:J61002 J61002] Strong promoter - gfp Tripart D103 (BV) - D131 (BI) August 1 MP131 L110 [http://partsregistry.org/Part:J61002 J61002] Medium promoter - gfp Tripart D124 (BV) - D131 (BI) August 1 MP132 L111 [http://partsregistry.org/Part:J61002 J61002] Weak promoter - ECFP D104 (BV) - D130 (BI) August 1 MP133 L112 [http://partsregistry.org/Part:J61002 J61002] Weak promoter - GFP tripart D104 (BV) - D131 (BI) August 1 MP134 L115 [http://partsregistry.org/Part:pSB1A2 pSB1A2] pLas - ECFP D105 (BV) - D130 (BI) August 1 MP135 L116 [http://partsregistry.org/Part:pSB1A2 pSB1A2] pLas - gfp Tripart D105 (BV) - D131 (BI) August 1 MP136 L117 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] yfp - Double Terminator D125 (FV) - D117 (FI) August 1 MP137 L118 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] rfp - Double Terminator D125 (FV) - D121 (FI) August 1 MP138 L119 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] lasR activator + LVA - Double Terminator D125 (FV) - D113 (FI) August 1 MP139 L121 [http://partsregistry.org/Part:pSB1A2 pSB1A2] pTet - gfp tripart D106 (BV) - D131 (BI) August 1 MP140 L124 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS - rfp D102 (BV) - D122 (BI) August 1 MP141 L125 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS - yfp D102 (BV) - D118 (BI) August 1 MP142 [http://partsregistry.org/Part:pSB3K3 pSB3K3] August 8 MP143.1 [http://partsregistry.org/Part:BBa_E0240 E0240] [http://partsregistry.org/Part:pSB1A2 pSB1A2] gfp generator 138 August 8 MP143.2 150 MP144.1 L128 [http://partsregistry.org/Part:J61002 J61002] pFlgA-RFP D132 (FI) - D136 (FV) MP144.3 ; MP144.4 127 August 12 MP144.2 166 MP144.3 163 MP144.4 154 MP145.1 L129 [http://partsregistry.org/Part:J61002 J61002] pFlgB-RFP D133 (FI) - D136 (FV) MP145.6 ; MP145.7 136 August 12 MP145.2 151 MP145.6 163 MP145.7 188 MP146.1 L130 [http://partsregistry.org/Part:J61002 J61002] pFlhB-RFP D134 (FI) - D136 (FV) MP146.1 ; MP146.2 298 August 12 MP146.2 142 MP146.7 170 MP146.8 143 MP147 L100 [http://partsregistry.org/Part:pSB1A2 pSB1A2] rbs TetR - ECFP D110 (BV) - D130 (BI) August 13 MP148.1 L101 [http://partsregistry.org/Part:pSB1A2 pSB1A2] rbs TetR - GFP tripart D110 (BV) - D131 (BI) DO NOT WORK !!!! MP148.2 ; MP148.3 109 August 13 MP148.2 123 MP148.3 123 MP149 L114 [http://partsregistry.org/Part:pSB2K3 pSB2K3] AracpBAD - gfp tripart D126 (BV) - D131 (BI) August 13 MP150 L120 [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressible promoter - ECFP D106 (BV) - D130 (BI) August 13 MP151 L122 [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS-lasI - ECFP D107 (BV) - D130 (BI) August 13 MP152 L123 [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS lasI - gfp tripart D107 (BV) - D131 (BI) August 13 MP153.1 L126 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI) MP153.1 ; MP153.3 100 August 13 MP153.2 131 MP153.3 113 MP154 L132 [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhDC D142(FV) - D139(FI) August 13 MP155 L133 [http://partsregistry.org/Part:pSB1A2 pSB1A2] OmpR* D142(FV) - D140(FI) WT sequence (August 19) August 13 ; August 19 MP156 L134 [http://partsregistry.org/Part:pSB1A2 pSB1A2] EnvZ* D142(FV) - D141(FI) lacks about 300 bp in EnvZ* (August 19) August 13 August 19 MP157 L138 [http://partsregistry.org/Part:pSB3K3 pSB3K3] gfp generator (E0240) D137(FV) - D138(FI) August 13 MP158 L139 [http://partsregistry.org/Part:pSB3K3 pSB3K3] E0240 RBS+ D140 (PstI) - D144 (ES) seems to have the plasmid without the insert and one with the insert August 15 MP159 L141 [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhD D142 (ES) - D145 (ES) has not been sequenced August 15 MP160 L142 [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhDC+promotor D143 (ES) - D145 (ES) does not have the insert August 15 MP161 L150 [http://partsregistry.org/Part:pSB1A2 pSB1A2] pLas + rbs-tetR-gfp tripart D105 (SP) - D146 (XP) incorrect clone -> to do again August 19 MP162.1 L151.1 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] rbs-lasR + double terminator D147 (ES) - D125 (EX) 151 August 19 MP162.2 172 MP162.3 149 MP163.1 [http://partsregistry.org/Part:BBa_B0032 B0032] [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS 80 August 20 MP163.2 79 MP164 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS+ ECFP+ LVA+ term August 20 MP165 [http://partsregistry.org/Part:BBa_E0430 E0430] [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS+ YFP+ LVA- term August 20 MP166 [http://partsregistry.org/Part:BBa_E0432 E0432] [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS+ YFP LVA+ term BUT be careful , it doesn't work. See registry August 20 MP167 [http://partsregistry.org/Part:BBa_E0420 E0420] [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS+ ECFP LVA- term ceci ne contient pas de LVA apparemment. ça correspondrait au ECFP normal de longue durée de vie August 20 MP168 [http://partsregistry.org/Part:BBa_I732078 I732078] [http://partsregistry.org/Part:pSB1A3 pSB1A3] RBS+ mRFP LVA+ term August 20 MP171 L167 [http://partsregistry.org/Part:pSB1A2 pSB1A2] gfp generator(E0240) - pTet gfp generator(E0240) - pTet August 25 MP172 L166 [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS(B0032) - tetR RBS(B0032) - tetR August 25 Digestion Products (Legend: Vector, Insert) Ø Digestion Products Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix Size BB Description Product in use []ng/µL Date D100.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26 D100.2 D101 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2076 15 10 July 29 D102.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 26 August 15 D102.2 D103.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 27 July 26 D103.2 35 D104.1 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26 D104.2 D105.1 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 26 D105.2 D106.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 8 July 26 D106.2 D107 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2750 14 August 23 5 12 July 26 July 23 D108.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 29 D108.2 D109 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 708 July 26 10 July 26 D110 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2768 14 August 23 20 8 19 July 26 August 23 August 28 D111.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 26 D111.2 D112 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2079 703 8 September 5 D113 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 779 20 July 26 D114 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 4003 778 20 July 26 D115.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29 D115.2 D116.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29 D116.1 D117 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI SpeI FI 3166 746 5 July 29 D118 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 3167 745 20 July 26 D119 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 743 10 July 26 D120 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 742 10 July 26 D121 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 704 10 July 26 D122 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 703 10 July 26 D123.1 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 26 July 26 D123.2 23 D124.1 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26 D124.2 D125.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 26 10 July 26 August 23 August 28 D125.2 24 3687 D126.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26 D126.2 D127.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26 D127.2 D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29 D128.1 D129.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29 D129.2 D130.1 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26 D130.2 D130.3 D131.1 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 8 July 26 D131.2 D131.3 D132 pFlgA - EcoRI SpeI FI - 250 5 August 6 D133 pFlgB - EcoRI SpeI FI - 250 5 August 6 D134 pFlhB - EcoRI SpeI FI - 249 5 August 6 D135 pFlhDC - EcoRI SpeI FI - problem August 6 D136 - [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI FV 2925 - August 6 August 16 D137 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FV 2727 - August 6 D138 E0240 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 899 - August 6 D139 flhDC - EcoRI PstI FI - 972 August 6 D140 E040 rbs+ - PstI PstI I - August 12 D141 flhD RBS - - EcoRI SpeI FI - August 12 D142 flhC RBS - - EcoRI SpeI FI - August 12 D143 flhDC + promoter - EcoRI SpeI FI - August 12 D144 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FI - August 12 D145 pSB1A2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - August 12 August 15 D146 MP148 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 2057 1609 rbs TetR - GFP tripart D110 (BV) - D131 (BI) August 15 D147 MP153 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 2056 799 Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI) August 15 D149 pfliL - EcoRI SpeI FI - August 14 August 16 D150 pfliL - XbaI PstI BI - August 14 August 16 D152 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI - August 14 D153 PCR 138 - gène flhDC - EcoRI SpeI FI - August 15 D154 PCR 140 - gène flhDC - EcoRI SpeI FI - August 15 D155 PCR 139 - pflhDC (URI) - EcoRI SpeI FI - August 15 D158 MP 155.1 - OmpR* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15 D159 MP 156.1 - EnvZ* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15 D161 MP 104 - pTet [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV - 39 August 16 D162 MP 114 - TetR [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - 11 August 16 D163 MP 143 - gfp generator [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 23 14 11 August 16 August 23 D164 MP 101 - pJ23101 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] SpeI PstI BV - 3 August 16 D165 MP162.2 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] XbaI PstI BI 2057 798 rbs-lasR - double terminator D147 (FI) - D125 (FV) 27 August 18 D166 MP 165.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV - 2942 bp RBS (B0034)+ YFP+ LVA- term B0015 59 August 26 September 3 D179 MP3.4 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] SpeI PstI BV B0015 (double terminator B0010-B0012) August 20 D180 MP101.1 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] SpeI PstI BV promoter J23101 August 20 D181 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV pTet (TetR repressible promoter) August 20 D182 MP114.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI TetR August 20 D183 MP119.3 - XbaI PstI BI pBad promoter August 20 D184 MP143.1 [http://partsregistry.org/Part:BBa_pSB2K3 pSB2K3] EcoRI SpeI FI gfp generator August 18 D185 MP163.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV B0032 RBS August 18 D187 MP168.1 [http://partsregistry.org/Part:BBa_pSB1A3 pSB1A3] EcoRI XbaI FV RBS (B0034)+ mRFP LVA+ term B0015 43 September 3 D188 PCR 138 - XbaI PstI Insert - 767 bp OmpR* August 26 D189 MP 101.2 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] XbaI PstI Vector - 2073 bp pSB1A2 August 26 D191 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2724 tetR 22 August 29 D192 MP144.2 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] EcoRI XbaI FV 2267 FlgA 26 August 29 D193 MP171 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 961 gfp generator(E0240) - pTet 9,5 August 29 D194 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 704 RBS(B0032) - tetR 13 August 29 D195 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 703 RBS(B0032) - tetR 11 August 29 D196 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2119 pTet 45 September 3 D197 MP157.2 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI 898 gfp generator(E0240) 12 September 3 D198 MP164.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2981 RBS (B0034)+ ECFP+ LVA+ term B0015 36 September 3 D199 MP167.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2942 RBS (B0034)+ ECFP LVA- term (B0015) 61 September 3 D200 MP171.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 960 gfp generator(E0240) - pTet 43 September 3 D201 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2724 tetR 13 August 29 D202 MP101.1 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] EcoRI nothing 3037 25 September 8 Strains (glycerol stock) Strains glycerol stock Number Name Biobricks Description Verified clone Date S103 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] AraC pBAD July 23 S106 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] ECFP (RBS+LVA+ Double Term) July 23 S107 [http://partsregistry.org/Part:BBa_B0034 B0034] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS (Efficiency = 1) July 23 S108 [http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] promoter pLas (lasr & PAI regulated) July 23 S109 [http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Strongest Constitutive promoter July 23 S110 [http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Weak Constitutive promoter July 23 S111 [http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressible promoter July 23 S112 [http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) ->LasI July 23 S113 [http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] B0034(rbs) -> TetR July 23 S114 [http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] lasR activator with LVA Tag July 23 S115 [http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] lasR activator July 23 S116 [http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] YFP July 23 S117 [http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] GFP July 23 S118 [http://partsregistry.org/Part:BBa_E1010 E1010] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] mRFP July 23 S119 OmpR M57I July 23 S120 EnvZ I281S July 23 S121 [http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressor July 23 S122 [http://partsregistry.org/Part:pSB1A3 pSB1A3] July 23 S123 L102 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong rbs - YFP D129 (BV) - D118 (BI) Aug 1 S124 L103 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong rbs - mRFP D129 (BV) - D122 (BI) Aug 1 S125 L104 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strong rbs - lasR activator D129 (BV) - D114 (BI) Aug 1 S126 L105 in [http://partsregistry.org/Part:J61002 J61002] Strong promoter - ECFP D123 (BV) - D130 (BI) Aug 1 S127 L106 in [http://partsregistry.org/Part:J61002 J61002] Strong promoter - gfp Tripart D123 (BV) - D131 (BI) Aug 1 S128 L107 in [http://partsregistry.org/Part:J61002 J61002] Strongest promoter - ECFP D103 (BV) - D130 (BI) Aug 1 S129 L108 in [http://partsregistry.org/Part:J61002 J61002] Strong promoter - GFP tripart D103 (BV) - D131 (BI) Aug 1 S130 L110 in [http://partsregistry.org/Part:J61002 J61002] Medium promoter - gfp tripart D124 (BV) - D131 (BI) Aug 1 S131 L111 in [http://partsregistry.org/Part:J61002 J61002] Weak promoter - ECFP D104 (BV) - D130 (BI) Aug 1 S132 L112 in [http://partsregistry.org/Part:J61002 J61002] Weak promoter - gfp Tripart D104 (BV) - D131 (BI) Aug 1 S133 L115 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] pLas - ECFP D105 (BV) - D130 (BI) Aug 1 S134 L116 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] pLas - gfp Tripart D105 (BV) - D131 (BI) Aug 1 S135 L117 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] yfp - Double Terminator D125 (FV) - D117 (FI) Aug 1 S136 L118 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] rfp - Double Terminator D125 (FV) - D121 (FI) Aug 1 S137 L119 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] LasR activator + LVA - Double terminator D125 (FV) - D113 (FI) Aug 1 S138 L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] pTet - gfp Tripart D106 (BV) - D131 (BI) Aug 1 S139 L124 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest rbs - mRFP D102 (BV) - D122 (BI) Aug 1 S140 L125 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest rbs - yfp D102 (BV) - D118 (BI) Aug 1 S141 MG1655 Aug 7 S142 [http://partsregistry.org/Part:J61002 J61002] Aug 7 S143 L128 in [http://partsregistry.org/Part:J61002 J61002] pFlgA-RFP D132 (FI) - D136 (FV) MP144.3 ; MP144.4 August 12 S144 L129 in [http://partsregistry.org/Part:J61002 J61002] pFlgB-RFP D133 (FI) - D136 (FV) MP145.6 ; MP145.7 August 12 S145 L130 in [http://partsregistry.org/Part:J61002 J61002] pFlhB-RFP D134 (FI) - D136 (FV) MP146.1 ; MP146.2 August 12 S146 L100 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] rbs TetR - ECFP D110 (BV) - D130 (BI) August 13 S147 L101 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] rbs TetR - GFP tripart D110 (BV) - D131 (BI) August 13 S148 L114 in [http://partsregistry.org/Part:pSB2K3 pSB2K3] AracpBAD - gfp tripart D126 (BV) - D131 (BI) August 13 S149 L120 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] tetR repressible promoter - ECFP D106 (BV) - D130 (BI) August 13 S150 L122 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS-lasI - ECFP D107 (BV) - D130 (BI) August 13 S151 L123 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS lasI - gfp tripart D107 (BV) - D131 (BI) August 13 S152 L126 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI) August 13 S153 L132 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhDC D142(FV) - D139(FI) August 13 S154 L133 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] OmpR* D142(FV) - D140(FI) August 13 S154 L133.1 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] OmpR* D142(FV) - D140(FI) WT sequence August 19 S155 L134 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] EnvZ* D142(FV) - D141(FI) August 13 S155 L134.2 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] EnvZ* D142(FV) - D141(FI) lacks about 300 bp in EnvZ* August 19 S156 L138 in [http://partsregistry.org/Part:pSB3K3 pSB3K3] gfp generator (E0240) D137(FV) - D138(FI) August 13 S157 E0240 GFP generator August 15 S158 pSB3K3 plasmid August 15 S159.1 L139.1 in in [http://partsregistry.org/Part:pSB3K3 pSB3K3] E0240 RBS+ D140 (PstI) - D144 (ES) seems to have the plasmid without the insert and one with the insert August 15 S159.2 incorrect clone S160.1 L141.1 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhD D142 (ES) - D145 (ES) has not been sequenced August 15 S160.2 has not been sequenced S160.3 has not been sequenced S161.1 L142.7 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhDC+promotor D143 (ES) - D145 (ES) does not seem to have the insert August 15 S161.2 incorrect clone S162 MP162.1 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] rbs-lasR + double terminator D147 (ES) - D125 (EX) August 18 S163 B0032 in pSB1A2 medium rbs (efficiency = 0,3) August 20 S164 E0422 strongest rbs ECFP LVA+ double terminator August 20 S165 E0430 strongest rbs YFP LVA- double terminator August 20 S166 E0432 strongest rbs YFP LVA+ double terminator August 20 S167 E0420 strongest rbs ECFP LVA- double terminator August 20 S168 I732078 strongest rbs mRFP LVA+ double terminator August 20

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