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Team:Chiba/Calendar-Home/6 September 2008
From 2008.igem.org
5 September 2008 <|> 7 September 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- PCR of BBa_I9030 and two control,BBa_F2620[http://partsregistry.org/Part:BBa_F2620](2007)).
DNA Template 1 dNTP mix 5 Foward Primer 5 Reverse Primer 5 DNA polymerase 1 Thermopol Buffer 5 dH2O 33 TOTAL 50μL
- 95℃,5min -> ( 95℃,1min -> 54℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃ store
- --->Gel Check
Sample DNA 1 Loading Dye 1 dH2O 4 TOTAL 6 - From left;
- 1 I9030 without LVA -> OK
- 2 I9030 Positive Control -> OK
- 3 F2620 Positive control -> OK
Gel Check Sample DNA : R0010
Sample DNA 1 Loading Dye 1 dH2O 4 TOTAL 6
- Sample DNA:
1:R0010,2:S03154,3:J04500,4:C0161,5:C0261
Sample No. 1 2 3 4 5 Sample DNA 1 1 1 1 1 PstⅠ 0.5 0.5 0.5 0.5 0.5 SpeⅠ 0.5 - 1 - - XbaⅠ - 0.5 - 0.5 0.5 Buffer 2 2 - 3 - - Buffer 3 - 6 - 6 6 (10×)BSA 2 0.6 3 - - (100×)BSA - - - 0.6 0.6 dH2O 3 6.4 7 1.4 1.4 TOTAL 20 60 30 60 60
Team:Output
no work
- From left;
