Team:Heidelberg/Notebook/Sensing Group/Notebook/6thweek

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Contents

1 Monday, 09/08/2008
2 Tuesdsay, 09/09/2008
3 Wednesday, 09/10/2008
4 Thursday, 09/11/2008
5 Friday, 09/12/2008

Monday, 09/08/2008

Liquid Culture of 5 colonies from LuxQ Transformation and 1 from F1 transformation
Miniprep of Liquid Culture from previous Transformation (done by Chenchen). Incubated for two days @ RT and about 6 h @ 37 °C
Digestion of the Miniprep products with xbaI (Buffer2 + BSA, 1 ul enzyme @ 37 °C about 40min)

Digestion of LuxQ with xbaI yielded expected bands (4736 + 1204 bp) for all samples, except for no. 4

Tuesdsay, 09/09/2008

Wednesday, 09/10/2008

Thursday, 09/11/2008

Friday, 09/12/2008

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