Team:Heidelberg/Notebook/Sensing Group/Notebook/6thweek

From 2008.igem.org

Revision as of 16:54, 26 October 2008 by Koljaschleich (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Back to the overview

Contents

1 Monday, 09/08/2008
2 Tuesdsay, 09/09/2008
3 Wednesday, 09/10/2008
4 Thursday, 09/11/2008
5 Friday, 09/12/2008

Monday, 09/08/2008

Liquid Culture of 5 colonies from LuxQ Transformation and 1 from F1 transformation
Miniprep of Liquid Culture from previous Transformation (done by Chenchen). Incubated for two days @ RT and about 6 h @ 37 °C
Digestion of the Miniprep products with xbaI (Buffer2 + BSA, 1 ul enzyme @ 37 °C about 40min)

Digestion of LuxQ with xbaI yielded expected bands (4736 + 1204 bp) for all samples, except for no. 4

Sequencing @ GATC yielded correct sequence for LuxQ no. 1

PCR for LuxQ and LuxP with Phusion Mastermix

PCR for LuxQ and LuxP. LuxQ PCR did not work, but first PCR for LuxP was positive.

Tuesdsay, 09/09/2008

Wednesday, 09/10/2008

Thursday, 09/11/2008

Friday, 09/12/2008

Retrieved from "http://2008.igem.org/Team:Heidelberg/Notebook/Sensing_Group/Notebook/6thweek"