Brown: Team Resistance/26 October 2008

26 October 2008

Biobricking work

• Made new culture of the PVJ4 transformation so that a miniprep can be done.
• New culture of gene S105 part made.

Began transforming promoters from the registry in order to ligate them to our cassette and create composite parts:

• pBAD prmoter
• IPTG inducible promoter
• Mercury inducible promoter
• Pu inducible promoter (xylene induced)
• T6 promoter, with activity modified by change in temperature

Also re-transformed ligations of the pVJ4 cassette and the S105 cassette (both transformations unsuccessful in the past)

• Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector. We should have seen two bands but we only saw one.

Conductivity Tests

• PVJ4 Colony 2 and 4 plus Controls. 10ml cultures. 420 ul of 50% Arabinose solution will be added. Conductance measurements will be taken before and after lysis has occurred.
• Throughout the test, Optical Density measurements will be taken.