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- 13:10, 28 October 2008 (diff | hist) Team:NTU-Singapore/Modelling/Stochastic Modeling (→Stochastic Model For E7 + Imm Production)
- 13:08, 28 October 2008 (diff | hist) Team:NTU-Singapore/Modelling/Stochastic Modeling (→Stochastic Modeling)
- 13:05, 28 October 2008 (diff | hist) Team:NTU-Singapore/Modelling/Stochastic Modeling (→Detection & Lysis System Version 2)
- 13:00, 28 October 2008 (diff | hist) Team:NTU-Singapore/Modelling/Stochastic Modeling (→Stochastic Model For Lysis Production)
- 12:49, 28 October 2008 (diff | hist) Team:NTU-Singapore/Modelling/Sensitivity Analysis (→Sensitivity Analysis) (top)
- 12:46, 28 October 2008 (diff | hist) Team:NTU-Singapore/Modelling/Deterministic Modeling (→Detection & Lysis System Version 1) (top)
- 12:44, 28 October 2008 (diff | hist) Team:NTU-Singapore/Modelling/Deterministic Modeling (→Detection & Lysis System Version 1)
- 12:44, 28 October 2008 (diff | hist) Team:NTU-Singapore/Modelling/Deterministic Modeling (→Detection & Lysis System Version 2 (with AND biological gate))
- 12:42, 28 October 2008 (diff | hist) Team:NTU-Singapore/Modelling/Sensitivity Analysis (→Lysis production Sensitivity Analysis)
- 12:41, 28 October 2008 (diff | hist) Team:NTU-Singapore/Modelling/Deterministic Modeling (→Updates)
- 12:39, 28 October 2008 (diff | hist) Team:NTU-Singapore/Modelling/Deterministic Modeling (→Updates)
- 12:38, 28 October 2008 (diff | hist) Team:NTU-Singapore/Modelling/Deterministic Modeling (→Lysis Production system)
- 22:35, 26 October 2008 (diff | hist) m Team:NTU-Singapore/Parts/Characterization of pLsrA-YFP
- 22:33, 26 October 2008 (diff | hist) N File:YFP time.png (top)
- 22:32, 26 October 2008 (diff | hist) N File:LsrA-YFP.jpg (top)
- 22:30, 26 October 2008 (diff | hist) m Team:NTU-Singapore/Wetlab/Experimental Results
- 22:20, 26 October 2008 (diff | hist) N File:OD time.png (top)
- 20:08, 26 October 2008 (diff | hist) m Team:NTU-Singapore/Wetlab/Materials and Equipment (→Preparation of AI-2)
- 20:08, 26 October 2008 (diff | hist) N File:AI2 OD.png (top)
- 20:07, 26 October 2008 (diff | hist) m Team:NTU-Singapore/Wetlab/Materials and Equipment (→Bacteria strains and media)
- 20:06, 26 October 2008 (diff | hist) N File:LuxS ko.png (top)
- 20:05, 26 October 2008 (diff | hist) m Team:NTU-Singapore/Wetlab/Materials and Equipment
- 17:45, 25 October 2008 (diff | hist) m Team:NTU-Singapore/Notebook/20 May 2008
- 17:44, 25 October 2008 (diff | hist) m Team:NTU-Singapore/Notebook/16 May 2008
- 17:44, 25 October 2008 (diff | hist) m Team:NTU-Singapore/Notebook/15 May 2008
- 17:44, 25 October 2008 (diff | hist) m Team:NTU-Singapore/Notebook/14 May 2008
- 17:43, 25 October 2008 (diff | hist) m Team:NTU-Singapore/Notebook/13 May 2008
- 17:43, 25 October 2008 (diff | hist) m Team:NTU-Singapore/Notebook/12 May 2008
- 17:43, 25 October 2008 (diff | hist) m Team:NTU-Singapore/Notebook/9 May 2008
- 17:42, 25 October 2008 (diff | hist) m Team:NTU-Singapore/Notebook/8 May 2008
- 17:42, 25 October 2008 (diff | hist) m Team:NTU-Singapore/Notebook/7 May 2008
- 17:41, 25 October 2008 (diff | hist) m Team:NTU-Singapore/Notebook/6 May 2008
- 17:41, 25 October 2008 (diff | hist) m Team:NTU-Singapore/Notebook/5 May 2008
- 17:39, 25 October 2008 (diff | hist) m Team:NTU-Singapore/Notebook
- 17:30, 25 October 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/20 May 2008 (New page: <div id="header">{{User:Greenbear/sandbox/header}}</div> <div id="maincontent" style="margin-top:100px;"> <html> <div id="arrow"> <a href="https://2008.igem.org/Team:NTU-Singapore/Notebook...)
- 17:27, 25 October 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/16 May 2008 (New page: <div id="header">{{User:Greenbear/sandbox/header}}</div> <div id="maincontent" style="margin-top:100px;"> <html> <div id="arrow"> <a href="https://2008.igem.org/Team:NTU-Singapore/Notebook...)
- 17:26, 25 October 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/15 May 2008 (New page: <div id="header">{{User:Greenbear/sandbox/header}}</div> <div id="maincontent" style="margin-top:100px;"> <html> <div id="arrow"> <a href="https://2008.igem.org/Team:NTU-Singapore/Notebook...)
- 17:24, 25 October 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/14 May 2008 (New page: <div id="header">{{User:Greenbear/sandbox/header}}</div> <div id="maincontent" style="margin-top:100px;"> <html> <div id="arrow"> <a href="https://2008.igem.org/Team:NTU-Singapore/Notebook...)
- 17:23, 25 October 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/13 May 2008 (New page: <div id="header">{{User:Greenbear/sandbox/header}}</div> <div id="maincontent" style="margin-top:100px;"> <html> <div id="arrow"> <a href="https://2008.igem.org/Team:NTU-Singapore/Notebook...)
- 17:22, 25 October 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/12 May 2008 (New page: <html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html> <div id="header">{{User:Greenbear/sandbox/header}}</div> <div id="maincontent"...)
- 17:21, 25 October 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/9 May 2008 (New page: <html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html> <div id="header">{{User:Greenbear/sandbox/header}}</div> <div id="maincontent"...)
- 17:19, 25 October 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/8 May 2008 (New page: <html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html> <div id="header">{{User:Greenbear/sandbox/header}}</div> <div id="maincontent"...)
- 17:18, 25 October 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/7 May 2008 (New page: <html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html> <div id="header">{{User:Greenbear/sandbox/header}}</div> <div id="maincontent"...)
- 17:17, 25 October 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/6 May 2008 (New page: <html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html> <div id="header">{{User:Greenbear/sandbox/header}}</div> <div id="maincontent"...)
- 17:14, 25 October 2008 (diff | hist) N Team:NTU-Singapore/Notebook/5 May 2008 (New page: <html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html> <div id="header">{{User:Greenbear/sandbox/header}}</div> <div id="maincontent"...)
- 17:00, 25 October 2008 (diff | hist) File:NTU new parts.png (uploaded a new version of "Image:NTU new parts.png") (top)
- 16:58, 25 October 2008 (diff | hist) m Team:NTU-Singapore
- 16:57, 25 October 2008 (diff | hist) File:NTU new parts.png (uploaded a new version of "Image:NTU new parts.png")
- 16:52, 25 October 2008 (diff | hist) N File:NTU new parts.png
- 16:41, 25 October 2008 (diff | hist) m Team:NTU-Singapore
- 17:25, 24 October 2008 (diff | hist) m Team:NTU-Singapore/Modelling/Parameter/LysisaD
- 14:22, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 14:09, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 14:08, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 14:03, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 13:51, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 13:29, 24 October 2008 (diff | hist) Team:NTU-Singapore/Parts/Fluorescence Microscope Image
- 13:27, 24 October 2008 (diff | hist) Team:NTU-Singapore/Acknowledgements
- 13:27, 24 October 2008 (diff | hist) Team:NTU-Singapore/Parts
- 13:23, 24 October 2008 (diff | hist) Team:NTU-Singapore/Project
- 13:09, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 12:46, 24 October 2008 (diff | hist) N File:Newparts3 hover.jpg (top)
- 12:30, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 12:28, 24 October 2008 (diff | hist) User:Greenbear/sandbox/header
- 13:31, 22 October 2008 (diff | hist) Team:NTU-Singapore/Acknowledgements (→Acknowledgements)
- 14:29, 14 October 2008 (diff | hist) m User:Greenbear/sandbox/header
- 16:57, 28 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/26 September 2008 (New page: =Friday 26 September= *Collecting the plates: Detection system and Production system were suscessfully transformed into competent LuxS mutants!!!)
- 07:49, 25 September 2008 (diff | hist) m Team:NTU-Singapore/Notebook/25 September 2008
- 03:35, 25 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/25 September 2008 (New page: =Thursday 25 September= *Minipreps for LsrA-YFP, followed by digestional gel check. *Make LuxS mutants more competent. *Grow W3110 in LB plus 0.8% glucose for 4 hours, centrifuge and take ...)
- 03:32, 25 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/24 September 2008 (New page: =Wednesday 24 September= *Collect plate from Tuesday: **LsrA-YFP: 1 colony *Inoculate LsrA into 5ml LBA; inoculate W3110 and LuxS mutants into 5ml LB each.)
- 03:29, 25 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/23 September 2008 (New page: =Tuesday 23 September= 930am-6pm: *Ligation of LsrA-RBS with [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0430 YFP generator]. *Transform detection and production system into L...)
- 08:09, 22 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/22 September 2008 (New page: =Monday 22 September= *Digestional gel check for miniprepped samples: 3xYFP generator (miniprepped last Friday by LuChao). **2 samples have the correct bands: 878bp insert and 2kb vector. ...)
- 06:48, 12 September 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/12 September 2008 (New page: =Friday 12 September:= *1200am: punching and dilution into TE buffer at 50 degrees C of [http://partsregistry.org/cgi/partsdb/dna.cgi?part_name=pSB2K3 pSB2K3] (for obtaining plasmid vector...)
- 17:46, 5 September 2008 (diff | hist) Team:NTU-Singapore/Acknowledgements
- 12:14, 2 September 2008 (diff | hist) m Team:NTU-Singapore/Notebook/2 September 2008 (→Tuesday 2 September)
- 12:13, 2 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/2 September 2008 (New page: =Tuesday 2 September= *Morning: Lu Chao: minipreps for 6 samples from Monday and prepared 1% gel. *Hung: digestional gel check.<br/>Results: **Among 2 samples of LacI-RBS-E7-Term, 1 sample...)
- 12:11, 2 September 2008 (diff | hist) N File:NTU gelcheck 0209.JPG (top)
- 12:06, 2 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/1 September 2008 (New page: =Monday 1 September= *Hung: inoculated: **2x LacI-RBS-E7-term colonies **2xLacI-RBS colonies (for minipreps) **2xLsrA-RBS-Lysis-Term (for minipreps))
- 12:04, 2 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/30 August 2008 (New page: =Saturday 30 August= *Hung: collect plates from Friday night: **The plate of LacI-RBS-E7-Term had 2 colonies. **The other 2 plates for minipreps purpose had many colonies. This also showed...)
- 11:55, 2 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/29 August 2008 (New page: =Friday 29 August= *Lu Chao: prepared new top10 competent cells (60x50ul tubes). *Hung: transformation into top10 for: **The remaining ligation mixture of LacI-RBS-E7-Term. **LacI-RBS (as ...)
- 11:50, 2 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/28 August 2008 (New page: =Thursday 28 August= *Lu Chao: inoculated 1 colony of Top10 cell into 5ml LB broth and incubated overnight at 37 degrees C. This is for the preparation of new top10 competent cells on Frid...)
- 11:47, 2 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/26 August 2008 (New page: =Tuesday 26 August= *Morning: Lu Chao: minipreps of 6 colonies inoculated on Monday and prepared 2% gel for later gel check. *Hung: digestional check:<br/>Results: **Among 4 samples of Lsr...)
- 11:40, 2 September 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/25 August 2008 (New page: =Monday 25 August= *Hung: inoculated: **2 colonies of LacI-RBS (V) - E7-Term(I) **4 colonies of LsrA-RBS (V) - Lysis-Term(I))
- 11:38, 2 September 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/23 August 2008 (New page: =Saturday 23 August= *Lu Chao: collected plates: **LacI-RBS(V)-E7-Term(I): 2 colonies **LacI-RBS(I)-E7-Term(V): no colony **LsrA-RBS(V)-Lysis-Term(I): 4 colonies)
- 11:36, 2 September 2008 (diff | hist) N File:NTU gelcheck 2608.JPG (top)
- 11:33, 2 September 2008 (diff | hist) m Team:NTU-Singapore/Notebook/22 August 2008
- 11:32, 2 September 2008 (diff | hist) N Team:NTU-Singapore/Notebook/22 August 2008 (New page: =Friday 22 August= *830am - 7pm : Hung, Lu Chao: digestion+ligation+transformation of: **LacI-RBS (vector, 2.2kb) with E7-Term (insert, 2.3kb). **LacI-RBS (insert, 250bp) with E7-Term (vec...)
- 11:31, 2 September 2008 (diff | hist) N File:NTU gel 2208.JPG (top)
- 11:54, 19 August 2008 (diff | hist) m Team:NTU-Singapore/Notebook/19 August 2008
- 11:50, 19 August 2008 (diff | hist) N File:NTU gel 19 08.JPG (E7-Term and Lysis-Term gel check) (top)
- 10:43, 19 August 2008 (diff | hist) m Team:NTU-Singapore/Notebook/19 August 2008 (→Tuesday 19 August)
- 10:10, 19 August 2008 (diff | hist) N Team:NTU-Singapore/Notebook/19 August 2008 (New page: =Tuesday 19 August= *800-930: Lu Chao: minipreps for 5xE7-term colonies and 7xlysis-term colonies. Prepare 2% gel. *1330-1400: Hung: digestion of the above plasmids with EcoRI and PstI for...)
- 10:23, 18 August 2008 (diff | hist) N Team:NTU-Singapore/Notebook/18 August 2008 (New page: =Monday 18 August= *Hung: 1545-1630: inoculate of 7xLysis-Term colonies and 5xE7-Term colonies.)
- 12:37, 17 August 2008 (diff | hist) Team:NTU-Singapore/Project/Results (→Testing)
- 12:28, 17 August 2008 (diff | hist) Team:NTU-Singapore/Project/Results (→Testing)
- 12:27, 17 August 2008 (diff | hist) Team:NTU-Singapore/Project/Results (→Testing)
- 08:13, 16 August 2008 (diff | hist) N Team:NTU-Singapore/Notebook/16 August 2008 (New page: =Saturday 16 August= *10am: Hung: collect the plates from Friday. **Plates of E7-Term had 3 colnies **Plates of Lysis-Term had 6 colonies **Plates of LsrA-Lysis had no colony :()
- 08:11, 16 August 2008 (diff | hist) N Team:NTU-Singapore/Notebook/15 August 2008 (New page: =Friday 15 August= Ligation of: #E7(I)-Terminator(V) #LsrA(V)-Lysis(I) #Lysis(V)-Terminator(I) *900-930:Hung: digestion of E7-I(E/S), Terminator-V(E/X), GFP (E/S) to compare the bands wit...)
- 17:41, 13 August 2008 (diff | hist) m Team:NTU-Singapore/Notebook/13 August 2008
- 07:32, 13 August 2008 (diff | hist) N Team:NTU-Singapore/Notebook/13 August 2008 (New page: =Wednesday 13 August= *8am: Lu Chao: minipreps for 6xLsrA-SV and 6xLysis-SV. *3pm-7pm: Hung, Lu Chao: digestional check.)
- 07:28, 13 August 2008 (diff | hist) N Team:NTU-Singapore/Notebook/12 August 2008 (New page: =Tuesday 12 August= *Lu Chao: inoculate 6xLysis-Standard Vector and 6xLsrA-Standard Vector)
- 08:21, 24 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/24 July 2008
- 08:06, 24 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/24 July 2008 (New page: =Thursday 24 July= *RESULT from Wednesday ligation and transformation: **E7-Term got 7 colonies!! **Lysis-standard vector and LsrA-standard vector got lots of colonies!! **Lysis-Term also...)
- 08:01, 24 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/23 July 2008 (New page: =Wednesday 23 July= Hung, Min: ==Digestion for Terminator with X/P on 2% gel== *As we could not see the band of Terminator insert on Tuesday to extract) *Gel loading and extraction + nanod...)
- 07:32, 24 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/22 July 2008
- 07:31, 24 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/22 July 2008
- 06:19, 24 July 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/22 July 2008 (New page: =Tuesday 22 July= Hung,Lu Chao, Min: ==Ligation of E7 (vector) with Terminator (insert)== *E7: cut with S/P for 2 hours *Terminator: cut with X/P for 2 hours)
- 06:16, 24 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/21 July 2008 (New page: =Monday 21 July= Hung, Lu Chao: ==Ligation of Lysis (insert) and Terminator (vector):== *For Terminator (V): cut with E/X in Buffer2 for 1 hour, and 2 hours (digestion time check) *For Lys...)
- 06:00, 24 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/19 July 2008 (New page: =Saturday 19 July= Darius: *Minipreps for E7 and T7ptag inoculated on Friday. *Run PCR for checking E7. *Inoculation for Lysis and LsrA cells.)
- 05:57, 24 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/18 July 2008 (New page: =Friday 18 July= *RESULTS FROM THURSDAY TRANSFORMATION: **Plate of E7 got 4 colonies!! **Plate of T7ptag got 8 colonies!! *We also received LsrA and Lysis (lyophilized form) in Bluescript ...)
- 05:52, 24 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/17 July 2008 (New page: =Thursday 17 July= *Ligate E7 and T7ptag(cut with E/P, gel extracted on Wednesday) into Empty vector. *MinElute PCR purification. *Transform into competent cells. (use all 10ul of each lig...)
- 05:48, 24 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/16 July 2008
- 05:11, 24 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/16 July 2008 (New page: =Wednesday 16 July= *Morning: We carried out several digestion: **GFP with E/P in buffer 2 for 1 hour; in buffer 2 for 2 hours; and in EcoRI buffer for 2 hours (this aims to compare the ef...)
- 11:34, 15 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/15 July 2008 (New page: *All 4 plates of Fe-GFP from Monday had colonies. We did the inoculation in the afternoon. *Ligation of T7ptag, E7 with empty vector from GFP: **We already got E7 (cut with E/P), E7 (cut w...)
- 11:18, 15 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/14 July 2008
- 11:12, 15 July 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/13 July 2008 (New page: *Darius: inoculation for E7-AmpR vector and T7ptag-AmpR vector.)
- 11:10, 15 July 2008 (diff | hist) Team:NTU-Singapore/Notebook/11 July 2008
- 15:47, 10 July 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/10 July 2008 (New page: =Thursday 10 July= *1015-1215: Hung, Lu Chao: minipreps and nanodrops for LacI-GFP 1ul (x3), LacI-GFP 4ul (x2), LacI-RBS 1:3, LacI-RBS 1:6, Fe-GFP 1ul(x3), Fe-GFP 4ul (x1). The microliter ...)
- 19:21, 9 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/9 July 2008
- 19:20, 9 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/9 July 2008
- 15:02, 9 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/9 July 2008
- 14:08, 8 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/8 July 2008
- 14:08, 8 July 2008 (diff | hist) Team:NTU-Singapore/Notebook/8 July 2008
- 16:24, 7 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/7 July 2008 (New page: =Monday 7 July= *Results from last week: **1 tube of LacI-RBS 34 was obtained! **Cells with LacI-RFP plasmids in fact appeared in pink color. And the color looks basically the same as the ...)
- 18:22, 5 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/5 July 2008
- 18:19, 5 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/5 July 2008 (New page: =Saturday 5 July= *Among the plates collected, only the plate of LacI-RFP has colonies. Plates with E7-empty plasmid and LacI-GFP do not have colony. *Lu Chao: minipreps for LacI-RBS 34. *...)
- 18:10, 5 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/4 July 2008 (New page: =Friday 4 July= *Hung, Darius: **Ligation of E7 into empty plasmid vector extracted from GFP plasmid. ***Gel electrophoresis for the E7 plasmids synthesized by PCR (with 2 blunt ends recog...)
- 17:56, 5 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/3 July 2008
- 17:42, 4 July 2008 (diff | hist) m Team:NTU-Singapore
- 15:03, 4 July 2008 (diff | hist) N File:NTU igem team members.JPG (top)
- 02:32, 3 July 2008 (diff | hist) Team:NTU-Singapore (→Graduate Student Advisors)
- 02:12, 3 July 2008 (diff | hist) Team:NTU-Singapore (→Team background)
- 11:32, 2 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/2 July 2008
- 11:30, 2 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/1 July 2008 (→Tuesday 1 July)
- 17:03, 1 July 2008 (diff | hist) m Team:NTU-Singapore
- 14:48, 1 July 2008 (diff | hist) m Team:NTU-Singapore/Acknowledgements
- 09:34, 1 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/30 June 2008
- 09:29, 1 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/1 July 2008
- 09:29, 1 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/1 July 2008
- 09:16, 1 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/1 July 2008
- 09:08, 1 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/1 July 2008 (New page: =Tuesday 1 July= *Hung, Darius, Choon Kit: try out ligation of pFe(insert) with GFP(vector); and pLacI(insert) with RBS34(vector): **1030-1130:prepare digestion mixtures for pFe,GFP,pLacI,...)
- 08:34, 1 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook
- 15:19, 30 June 2008 (diff | hist) m Team:NTU-Singapore (→Project introduction)
- 16:51, 28 June 2008 (diff | hist) m Team:NTU-Singapore
- 16:50, 28 June 2008 (diff | hist) m Team:NTU-Singapore
- 16:48, 28 June 2008 (diff | hist) N File:NTU logo ver3.jpg (top)
- 16:43, 28 June 2008 (diff | hist) m Team:NTU-Singapore
- 16:32, 28 June 2008 (diff | hist) N File:NTU logo ver3.gif (NTU logo version 3) (top)
- 05:12, 28 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/28 June 2008 (New page: =Saturday 28 June= *Hung: 10:30am to 12:30 pm: miniprep for 6 samples of pFe-GFP)
- 14:10, 27 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/27 June 2008 (New page: =Saturday 27 June= *Hung: miniprep for pFe,Terminator,RBS32,RBS34,pT7,GFP,empty plasmid AmpR,LacI *Choon Kit: inoculate pFe-GFP)
- 16:34, 26 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/26 June 2008 (New page: =Thursday 26 June= ==10:30am to 12:30pm:== *Min-Lin, Hung: miniprep (with new kit) for: **GFP self-ligated plasmid (control) **GFP producer E0480 *Choon Kit: nanodrop for digested Fe, GFP ...)
- 16:13, 26 June 2008 (diff | hist) m User:Greenbear/sandbox/header
- 16:12, 26 June 2008 (diff | hist) N File:NTU Banner.gif (top)
- 16:11, 26 June 2008 (diff | hist) m User:Greenbear/sandbox/header
- 15:58, 26 June 2008 (diff | hist) File:NTU Banner2.jpg (uploaded a new version of "Image:NTU Banner2.jpg") (top)
- 15:57, 26 June 2008 (diff | hist) N File:NTU Banner2.jpg
- 13:17, 25 June 2008 (diff | hist) m User:Greenbear/sandbox/header
- 13:03, 25 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/25 June 2008
- 04:48, 25 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/25 June 2008
- 04:15, 25 June 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/25 June 2008 (New page: =Wednesday 25 June=)
- 04:07, 25 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/24 June 2008
- 03:33, 25 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/24 June 2008
- 03:25, 25 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/24 June 2008
- 16:31, 23 June 2008 (diff | hist) N File:NTU Banner copy.jpg (top)
- 16:27, 23 June 2008 (diff | hist) File:NTU IGEM banner.jpg (uploaded a new version of "Image:NTU IGEM banner.jpg") (top)
- 16:25, 23 June 2008 (diff | hist) N File:NTU IGEM banner.jpg (2nd version of NTU iGEM team banner)
- 08:57, 23 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/23 June 2008
- 08:56, 23 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/23 June 2008 (New page: =Monday 23 June= ==Morning (9am-1230pm) *DNA extraction (using Miniprep kit) for 21 samples that Choon Kit inoculated on [[https://2008.igem.org/wiki/index.php?title=Team:NTU-Singapore/Note...)
- 08:49, 23 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/22 June 2008 (New page: =Sunday 22 June= ChoonKit (4-6 pm): Inoculate the following samples: *5 x E7-empty plasmid *5 x supD-empty plasmid *5 x T7ptag-empty plasmid *5 x Fe-GFP *1 x AmpR empty plasmid)
- 08:45, 23 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/21 June 2008
- 08:45, 23 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/20 June 2008 (→Afternoon:)
- 08:45, 23 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/21 June 2008 (New page: =Saturday 21 June= Come and collect the agar plates from 37 degrees C incubator, then put into 4 degrees C fridge. *E7-Empty plasmid, SupD-empty plasmid, T7ptag-empty plasmid have dozens o...)
- 08:34, 23 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/20 June 2008 (New page: =Friday 20 June= ==Morning:== *Miniprep for pLacI, followed by DNA concentrating using QiAQuick PCR purification kit. ==Afternoon:== *Transformation and cell cloning 4 empty plasmids from ...)
- 07:25, 23 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/19 June 2008 (New page: =Thursday 19 June= *Miniprep for SupD & T7ptag *Afternoon: these tasks are carried out for LacI promoter and supD: **Gel extraction **Ligation **Cloning **Inoculate overnight at 37 degrees...)
- 07:22, 23 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/18 June 2008 (New page: =Wednesday 18 June= These tasks are carried out for E7 insert and empty plasmid vector for RBS 0034: *4:30 pm: 1st digestion *6:30 pm: PCR purification, followed by 2nd digestion *11:30 pm...)
- 07:16, 23 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/17 June 2008 (New page: =Tuesday 17 June= *9 am to 2:30pm:We take out 25 cryotubes incubated at 37 degrees C from Monday night: **1 x Special one E7 **4 x GFP **4 x RBS 0032 **4 x RBS 0034 **4 x Fe promoter **4 x...)
- 11:30, 19 June 2008 (diff | hist) m Team:NTU-Singapore
- 11:29, 19 June 2008 (diff | hist) m Team:NTU-Singapore
- 11:26, 19 June 2008 (diff | hist) Nm User:Greenbear/sandbox2 (New page: <html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"> <script src="http://greenbear88.googlepages.com/SpryMenuBar.js" type="text/javascript">...) (top)
- 09:09, 19 June 2008 (diff | hist) m User:Greenbear/sandbox/header
- 08:39, 19 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook
- 08:37, 19 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook
- 07:48, 19 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook
- 05:52, 19 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook
- 05:51, 19 June 2008 (diff | hist) m Team:NTU-Singapore
- 05:47, 19 June 2008 (diff | hist) m User:Greenbear/sandbox/header
- 05:29, 19 June 2008 (diff | hist) m Team:NTU-Singapore/Project
- 05:27, 19 June 2008 (diff | hist) m Team:NTU-Singapore/Project
- 05:19, 19 June 2008 (diff | hist) m User:Greenbear/sandbox/header
- 16:32, 18 June 2008 (diff | hist) m Team:NTU-Singapore (→Project introduction)
- 16:24, 18 June 2008 (diff | hist) N File:NTU team.jpg (top)
- 16:20, 18 June 2008 (diff | hist) File:Xdx29.tmp.jpg (uploaded a new version of "Image:Xdx29.tmp.jpg") (top)
- 10:22, 18 June 2008 (diff | hist) m Team:NTU-Singapore/Project (→Introduction)
- 10:21, 18 June 2008 (diff | hist) Team:NTU-Singapore/Project (→Introduction)
- 10:19, 18 June 2008 (diff | hist) m Team:NTU-Singapore/Acknowledgements (→Acknowledgements)
- 10:18, 18 June 2008 (diff | hist) Team:NTU-Singapore/Acknowledgements (→Acknowledgements)
- 05:43, 16 June 2008 (diff | hist) m Team:NTU-Singapore/Project/Design (→O157:H7 full detector system)
- 05:42, 16 June 2008 (diff | hist) N File:Detector AND gate.jpg (top)
- 05:31, 16 June 2008 (diff | hist) Team:NTU-Singapore/Project/Design (→Construct design)
- 05:29, 16 June 2008 (diff | hist) N File:Production part NTU.jpg (This is the part of producing colicin E7 and immunity protein in response to the presence of lactose/IPTG.) (top)
- 08:33, 13 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/11 June 2008 (→Wednesday 11 June)
- 08:24, 13 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/10 June 2008 (→Tuesday 10 June)
- 08:23, 13 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/10 June 2008 (→Tuesday 10 June)
- 08:09, 13 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/11 June 2008 (→Wednesday 11 June)
- 08:01, 13 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/11 June 2008 (→Wednesday 11 June)
- 07:44, 13 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/11 June 2008 (→Wednesday 11 June)
- 07:35, 13 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/11 June 2008 (→Wednesday 11 June)
- 07:09, 13 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/11 June 2008 (→Wednesday 11 June)
- 05:08, 13 June 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/11 June 2008 (New page: =Wednesday 11 June= *Morning: **Choon Kit, Hung: **#Gel electrophoresis for overnight digested products. Please refer to [https://2008.igem.org/wiki/index.php?title=Team:NTU-Singapore/Note...)
- 05:03, 13 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/10 June 2008 (→Tuesday 10 June)
- 04:50, 13 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/10 June 2008 (→Tuesday 10 June)
- 04:25, 13 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/10 June 2008 (→Tuesday 10 June)
- 04:25, 13 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/10 June 2008 (→Tuesday 10 June)
- 03:35, 10 June 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/10 June 2008 (New page: =Tuesday 10 June= *Morning: **Choon Kit, Hung: digestion of BBa_B0015 terminator and E7 plasmid with SpeI and XbaI. {|border="1" | |BBa_B0015 Terminator |E7 |- |DNA |5 |30 |- |Buffer 2 |1 ...)
- 02:20, 10 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/7 June 2008 (New page: =Saturday 7 June= *Hung: **Take out from the incubator the 6 cryotubes (GFP producer x2,RBS 0032 x2,RBS 0034 x2) =>Spin down =>Pour out the liquid =>Store at -20 oC fridge. **Take out from...)
- 02:11, 10 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/9 June 2008 (→Monday 9 June)
- 01:58, 10 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/9 June 2008 (notebook update)
- 03:06, 9 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/6 June 2008 (→Lab details (to be edited))
- 02:59, 9 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/5 June 2008 (→Lab details)
- 18:13, 8 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/6 June 2008 (New page: =Friday 6 June= ==Lab details (to be edited)== Hung,Choonkit: *Morning: **Take out the PCR samples **Prepare digestion solutions for T7, GFP producer, RBS 0032 and 0034, Immunity 1 and 2, ...)
- 18:08, 8 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/5 June 2008
- 18:07, 8 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/5 June 2008 (New page: =Thursday 5 June= ==Lab details== *Chin Chong, Darius: PCR (updated later) *Hung, Choon Kit: **Inoculation for GFP generator, RBS 0034, T7 promoter **Re-do the amplification of GFP genera...)
- 17:59, 8 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/4 June 2008
- 17:59, 8 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/3 June 2008 (→Lab details)
- 17:56, 8 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/3 June 2008 (→Lab details)
- 17:56, 8 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/3 June 2008
- 17:50, 8 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/2 June 2008
- 17:37, 8 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/4 June 2008
- 17:32, 8 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/4 June 2008 (New page: =Wednesday 4 June= ==Lab details:== *Morning: **Take out 4 cryotubes (2 terminators, 2 LacI-GFP) from yesterday incubation, then spin them down, pour out the liquid to obtain cell pellets....)
- 17:06, 8 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/3 June 2008
- 15:16, 8 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/2 June 2008
- 15:09, 8 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/2 June 2008
- 14:49, 8 June 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/2 June 2008 (New page: =Monday 2nd June= ==Hung(~~~), Zhen Fu== *)
- 12:59, 8 June 2008 (diff | hist) Team:NTU-Singapore/Modeling
- 09:42, 5 June 2008 (diff | hist) Team:NTU-Singapore/Acknowledgements
- 08:39, 2 June 2008 (diff | hist) Team:NTU-Singapore/Team (update team profile)
- 08:33, 2 June 2008 (diff | hist) N File:Teamprofile NTU.jpg (The profiles of NTU iGEM team members) (top)
- 03:46, 2 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/30 May 2008
- 03:45, 2 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/30 May 2008 (→Friday 30 May 2008)
- 03:34, 2 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/30 May 2008 (New page: =Friday 30 May 2008= *Hung (~~~) and Choon Kit: Analysis of Fe promoter plasmid by running Gel Electrophoresis (the remaining steps of phase III).)
- 03:28, 2 June 2008 (diff | hist) Team:NTU-Singapore/Notebook/29 May 2008 (→Wednesday)
- 03:23, 2 June 2008 (diff | hist) m Team:NTU-Singapore/Notebook/28 May 2008
- 04:02, 30 May 2008 (diff | hist) Team:NTU-Singapore/Notebook/28 May 2008
- 03:55, 30 May 2008 (diff | hist) Team:NTU-Singapore/Notebook/27 May 2008
- 03:50, 30 May 2008 (diff | hist) Team:NTU-Singapore/Notebook/27 May 2008 (→Observations:)
- 03:50, 30 May 2008 (diff | hist) Team:NTU-Singapore/Notebook/27 May 2008 (→Notes:)
- 03:49, 30 May 2008 (diff | hist) Team:NTU-Singapore/Notebook/27 May 2008 (→Conclusion:)
- 03:49, 30 May 2008 (diff | hist) Team:NTU-Singapore/Notebook/27 May 2008 (→Observations:)
- 03:44, 30 May 2008 (diff | hist) N File:IMG 0365.JPG (LacI miniprep result. Yeah!) (top)
- 02:52, 30 May 2008 (diff | hist) Team:NTU-Singapore/Notebook/27 May 2008 (→Phase III:)
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