User contributions
From 2008.igem.org
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- 02:45, 30 October 2008 (diff | hist) Team:Waterloo/Notebook/Oligos
- 02:42, 30 October 2008 (diff | hist) Team:Waterloo/Notebook/Oligos
- 02:40, 30 October 2008 (diff | hist) Team:Waterloo/Notebook/Oligos
- 02:38, 30 October 2008 (diff | hist) Team:Waterloo/Notebook/Oligos
- 02:35, 30 October 2008 (diff | hist) Team:Waterloo/Parts
- 02:34, 30 October 2008 (diff | hist) Team:Waterloo/Parts
- 02:31, 30 October 2008 (diff | hist) N File:PmeIR.PNG (top)
- 02:31, 30 October 2008 (diff | hist) N File:Prfp.PNG (top)
- 02:31, 30 October 2008 (diff | hist) N File:POCI rfp.PNG (top)
- 02:31, 30 October 2008 (diff | hist) N File:PrecA rfp.PNG (top)
- 02:30, 30 October 2008 (diff | hist) N File:C0052R.PNG (top)
- 02:30, 30 October 2008 (diff | hist) Team:Waterloo/Parts
- 02:29, 30 October 2008 (diff | hist) N File:C0051R.PNG (top)
- 02:28, 30 October 2008 (diff | hist) N File:RfpR.PNG (top)
- 02:28, 30 October 2008 (diff | hist) Team:Waterloo/Parts
- 01:56, 30 October 2008 (diff | hist) N File:RPlacTT.PNG (top)
- 01:56, 30 October 2008 (diff | hist) Team:Waterloo/Parts
- 01:51, 30 October 2008 (diff | hist) File:RTTPOCI.PNG (uploaded a new version of "Image:RTTPOCI.PNG") (top)
- 01:51, 30 October 2008 (diff | hist) N File:RTTPOCI.PNG
- 01:50, 30 October 2008 (diff | hist) Team:Waterloo/Parts
- 01:11, 30 October 2008 (diff | hist) File:Headless Chicken.PNG (uploaded a new version of "Image:Headless Chicken.PNG") (top)
- 01:10, 30 October 2008 (diff | hist) N File:Headless Chicken.PNG
- 01:09, 30 October 2008 (diff | hist) Team:Waterloo/Team
- 01:08, 30 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 01:06, 30 October 2008 (diff | hist) N File:EngSoc.PNG (top)
- 01:05, 30 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 01:05, 30 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 20:47, 29 October 2008 (diff | hist) N Waterloo/30 September 2008 (New page: <u>08/09/30 Tue</u><br> [x] Ligation for C0051'' Plac XO, C0051'', and rfp' XO into pJET<br> [x] Transformation of above ligation<br> [x] Check gel of 09/29 GEx<br> [x] PCR of rfp'+TT<b...) (top)
- 20:42, 29 October 2008 (diff | hist) N Waterloo/29 September 2008 (New page: <u>08/09/29 Mon</u><br> [x] Digest to subclone into pSB2K3<br> [x] Gel<br> [x] Gel extract<br>) (top)
- 20:37, 29 October 2008 (diff | hist) Waterloo/27 September 2008 (top)
- 20:37, 29 October 2008 (diff | hist) N Waterloo/27 September 2008 (New page: <u>08/09/27 Sat</u> [x] check gel of PCR from 09/26 [x] Miniprep PCR products)
- 20:33, 29 October 2008 (diff | hist) N Waterloo/26 September 2008 (New page: <u>08/09/26 Fri</u> <br> [x] Colony PCR of C0051'' and PmeIGene6 patches<br> [x] Make competent cells (TG1)<br>) (top)
- 20:29, 29 October 2008 (diff | hist) N Waterloo/25 September 2008 (New page: <u>08/09/25 Thu</u><br> [x] Inoculation of lasR, C0051'', PmeI' Gene6'<br> [x] Miniprep lasR to get pSB2K3<br> [x] Full digest (E/S) of Gene6', PmeI', C0051', pSB2K3(lasR)<br> [x] Gel of ...) (top)
- 20:24, 29 October 2008 (diff | hist) Waterloo/24 September 2008 (top)
- 20:23, 29 October 2008 (diff | hist) N Waterloo/24 September 2008 (New page: <u>08/09/24 Wed</u> [x] Test gel of 09/23 PCR [x] GEx of 09/23 PCR)
- 20:21, 29 October 2008 (diff | hist) N Waterloo/23 September 2008 (New page: <u>08/09/23</u> <br> [x] Ligation of 09/21 GEx<br> [x] Transformation<br> [x] XO PCR to construct Plac+C0051'', C0051'', rfp''<br> [x] Inoculation of stuff to send for sequencing<br>) (top)
- 20:13, 29 October 2008 (diff | hist) N Waterloo/21 September 2008 (New page: <u>08/09/21 Sun</u><br> [x] Gel of XO PCR from 09/20<br> [x] Excise good bands<br>) (top)
- 20:11, 29 October 2008 (diff | hist) N Waterloo/20 September 2008 (New page: <u>08/09/20 Sat</u> [x] XO PCR to make PmeI'+T7Gene6' and C0051'') (top)
- 20:08, 29 October 2008 (diff | hist) N Waterloo/22 September 2008 (New page: <u>08/09/22 Mon</u><br> [x] Colony PCR of pJET clones<br> [x] Run gel of colony PCR<br> [x] GEx of 9/21 gel<br> [x] Transformation<br>) (top)
- 20:01, 29 October 2008 (diff | hist) N Waterloo/19 September 2008 (New page: <u>08/09/19 Fri</u> <br> [x] Check gel of Plac PCRs on 1.2% gel to see if it is separable from primer cloud<br> [x] Gel extraction<br> [x] Ligation into pJET<br> [x] Transformation of pJE...) (top)
- 19:36, 29 October 2008 (diff | hist) Waterloo/29 October 2008 (top)
- 19:35, 29 October 2008 (diff | hist) N Waterloo/29 October 2008 (New page: <u>08/10/29 Wed</u> [] inoculate (in duplicate) all parts with good sequences to make stock [] make glycerol stock of all good parts - to be done at a later date (after inoculation) [] wo...)
- 19:32, 29 October 2008 (diff | hist) N Waterloo/28 October 2008 (New page: <u>08/10/28 Tue</u><br> [x] ship parts to MIT!<br> [] inoculate (in duplicate) all parts with good sequences to make stock<br> - will be done at a later date<br> [x] autoclave liquid wast...) (top)
- 19:23, 29 October 2008 (diff | hist) Waterloo/27 October 2008 (top)
- 19:23, 29 October 2008 (diff | hist) N Waterloo/27 October 2008 (New page: <u>08/10/27 Mon</u> Submissions must be POSTMARKED by the 29th! [x] Prepare samples for submission [x] check sequencing results!! [x] make more liquid LB media in 5 mL tubes [x] work on ...)
- 19:19, 29 October 2008 (diff | hist) Waterloo/26 October 2008 (top)
- 19:19, 29 October 2008 (diff | hist) N Waterloo/26 October 2008 (New page: <u>08/10/26 Sun</u> [x] subculture inoculations from Friday [x] inoculation of PrecA-rfp' transformaion in TG1)
- 19:16, 29 October 2008 (diff | hist) Waterloo/24 October 2008 (top)
- 19:15, 29 October 2008 (diff | hist) N Waterloo/24 October 2008 (New page: <u>08/10/24 Fri</u> [] pestering Scott/neils about protocols for plate reader [] based on sequencing results, prepare samples to ship on Monday (including putative PrecA-rfp' preps!) http...)
- 17:58, 29 October 2008 (diff | hist) Team:Waterloo (→Gold Level Support Provided By)
- 17:56, 29 October 2008 (diff | hist) Team:Waterloo
- 17:54, 29 October 2008 (diff | hist) Team:Waterloo
- 17:49, 29 October 2008 (diff | hist) Team:Waterloo
- 17:26, 29 October 2008 (diff | hist) Team:Waterloo/Notebook/Oligos
- 16:50, 29 October 2008 (diff | hist) Team:Waterloo/Notebook/Oligos
- 16:48, 29 October 2008 (diff | hist) Team:Waterloo/Notebook/Oligos
- 16:38, 29 October 2008 (diff | hist) N File:Robyn Halbot.PNG (top)
- 16:36, 29 October 2008 (diff | hist) N Team:Waterloo/Notebook/Oligos (New page: {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" !align="center"|Home !align="cent...)
- 16:34, 29 October 2008 (diff | hist) Team:Waterloo/Notebook (→Oligos)
- 16:33, 29 October 2008 (diff | hist) Team:Waterloo/Notebook
- 15:36, 29 October 2008 (diff | hist) N File:Erica Tiberia.PNG (top)
- 14:31, 29 October 2008 (diff | hist) Team:Waterloo/Team
- 14:25, 29 October 2008 (diff | hist) N File:Jordan LaPointe.PNG (top)
- 14:23, 29 October 2008 (diff | hist) N File:Ben Ho.PNG (top)
- 14:21, 29 October 2008 (diff | hist) N File:Maria Trainer.PNG (top)
- 14:16, 29 October 2008 (diff | hist) N File:Scott Clark.PNG (top)
- 14:11, 29 October 2008 (diff | hist) File:John Heil.PNG (uploaded a new version of "Image:John Heil.PNG") (top)
- 14:09, 29 October 2008 (diff | hist) N File:Nick Fischer.PNG (top)
- 14:07, 29 October 2008 (diff | hist) N File:John Heil.PNG
- 13:59, 29 October 2008 (diff | hist) N File:Jostenn Herritt.PNG (top)
- 13:53, 29 October 2008 (diff | hist) N File:Somil Bhargava.PNG (top)
- 13:34, 29 October 2008 (diff | hist) N File:Hillary Yeung.PNG (top)
- 13:32, 29 October 2008 (diff | hist) Team:Waterloo/Team
- 13:29, 29 October 2008 (diff | hist) Team:Waterloo/Team
- 13:25, 29 October 2008 (diff | hist) N File:Andre Masella.PNG (top)
- 13:24, 29 October 2008 (diff | hist) Team:Waterloo/Team
- 01:14, 29 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 23:28, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 23:27, 28 October 2008 (diff | hist) N File:DeptAppMath.PNG (top)
- 23:25, 28 October 2008 (diff | hist) N File:WEEF.PNG (top)
- 23:24, 28 October 2008 (diff | hist) N File:Sarstedt.PNG (top)
- 23:24, 28 October 2008 (diff | hist) N File:FisherSci.PNG (top)
- 23:20, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 22:55, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Competent Cells (top)
- 22:54, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Transformation (top)
- 22:54, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Inoculation (top)
- 22:54, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation (top)
- 22:54, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Ligation (top)
- 22:53, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Gel Electrophoresis (top)
- 22:53, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/DNA Extraction (top)
- 22:52, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/DNA Digestion (top)
- 22:51, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Colony PCR (top)
- 22:51, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes (top)
- 22:51, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Miniprep (top)
- 22:50, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Daily Log (top)
- 22:50, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Daily Log
- 22:37, 28 October 2008 (diff | hist) N File:Brendan McConkey.PNG (top)
- 22:36, 28 October 2008 (diff | hist) N File:Josh Neufeld.PNG (top)
- 22:34, 28 October 2008 (diff | hist) N File:Marc Aucoin.PNG (top)
- 22:33, 28 October 2008 (diff | hist) N File:Trevor Charles.PNG (top)
- 22:31, 28 October 2008 (diff | hist) N File:Barb Moffatt.PNG (top)
- 22:29, 28 October 2008 (diff | hist) N File:Brian Ingalls.PNG (top)
- 22:25, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Daily Log
- 22:25, 28 October 2008 (diff | hist) Team:Waterloo/Notebook
- 22:24, 28 October 2008 (diff | hist) Team:Waterloo/Notebook (→Daily Log)
- 22:23, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Daily Log
- 22:22, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Daily Log (New page: <b><u>Lab Calendar</u></b> <center> <tr><td> {{#calendar: title=Waterloo |year=2008 | month=05}} <td><td> {{#calendar: title=Waterloo |year=2008 | month=06}} <td><td> {{#cal...)
- 22:21, 28 October 2008 (diff | hist) Team:Waterloo/Notebook
- 21:26, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes
- 21:22, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes
- 21:22, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes
- 21:20, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes
- 21:18, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes
- 21:17, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Making SOB Media (Removing all content from page) (top)
- 21:17, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes
- 21:16, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Making LB Media (Removing all content from page) (top)
- 21:16, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes
- 21:15, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Making AI Media (Removing all content from page) (top)
- 21:14, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Recipes (New page: * Making Auto Induction Media * Making LB Media * [[Team:Waterloo/Notebook/Making SOB Media|Making SOB...)
- 21:13, 28 October 2008 (diff | hist) Team:Waterloo/Notebook
- 21:08, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Competent Cells (Team:Waterloo/Notebook/Competent Cells moved to Team:Waterloo/Notebook/Protocols/Competent Cells) (top)
- 21:08, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Competent Cells (Team:Waterloo/Notebook/Competent Cells moved to Team:Waterloo/Notebook/Protocols/Competent Cells)
- 21:07, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Transformation (Team:Waterloo/Notebook/Transformation moved to Team:Waterloo/Notebook/Protocols/Transformation) (top)
- 21:07, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Transformation (Team:Waterloo/Notebook/Transformation moved to Team:Waterloo/Notebook/Protocols/Transformation)
- 21:06, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Inoculation (Team:Waterloo/Notebook/Inoculation moved to Team:Waterloo/Notebook/Protocols/Inoculation) (top)
- 21:06, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Inoculation (Team:Waterloo/Notebook/Inoculation moved to Team:Waterloo/Notebook/Protocols/Inoculation)
- 21:06, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Glycerol Stock Preparation (Team:Waterloo/Notebook/Glycerol Stock Preparation moved to Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation) (top)
- 21:06, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation (Team:Waterloo/Notebook/Glycerol Stock Preparation moved to Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation)
- 21:05, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Ligation (Team:Waterloo/Notebook/Ligation moved to Team:Waterloo/Notebook/Protocols/Ligation) (top)
- 21:05, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Ligation (Team:Waterloo/Notebook/Ligation moved to Team:Waterloo/Notebook/Protocols/Ligation)
- 21:04, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Gel Electrophoresis (Team:Waterloo/Notebook/Gel Electrophoresis moved to Team:Waterloo/Notebook/Protocols/Gel Electrophoresis) (top)
- 21:04, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Gel Electrophoresis (Team:Waterloo/Notebook/Gel Electrophoresis moved to Team:Waterloo/Notebook/Protocols/Gel Electrophoresis)
- 21:04, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/DNA Extraction (Team:Waterloo/Notebook/DNA Extraction moved to Team:Waterloo/Notebook/Protocols/DNA Extraction) (top)
- 21:04, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/DNA Extraction (Team:Waterloo/Notebook/DNA Extraction moved to Team:Waterloo/Notebook/Protocols/DNA Extraction)
- 21:02, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/DNA Digestion (Team:Waterloo/Notebook/DNA Digestion moved to Team:Waterloo/Notebook/Protocols/DNA Digestion) (top)
- 21:02, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/DNA Digestion (Team:Waterloo/Notebook/DNA Digestion moved to Team:Waterloo/Notebook/Protocols/DNA Digestion)
- 21:02, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Colony PCR (Team:Waterloo/Notebook/Colony PCR moved to Team:Waterloo/Notebook/Protocols/Colony PCR) (top)
- 21:02, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Colony PCR (Team:Waterloo/Notebook/Colony PCR moved to Team:Waterloo/Notebook/Protocols/Colony PCR)
- 21:01, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols (top)
- 21:00, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Miniprep (Team:Waterloo/Notebook/Miniprep moved to Team:Waterloo/Notebook/Protocols/Miniprep) (top)
- 21:00, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Miniprep (Team:Waterloo/Notebook/Miniprep moved to Team:Waterloo/Notebook/Protocols/Miniprep)
- 20:59, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors/Protocols (Removing all content from page) (top)
- 20:58, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols (New page: {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" !align="center"|Home !align="cent...)
- 20:58, 28 October 2008 (diff | hist) Team:Waterloo/Notebook
- 20:57, 28 October 2008 (diff | hist) N Team:Waterloo/Sponsors/Protocols (New page: {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" !align="center"|Home !align="cent...)
- 20:56, 28 October 2008 (diff | hist) Team:Waterloo/Notebook
- 20:23, 28 October 2008 (diff | hist) N File:Fermentas.PNG (top)
- 20:22, 28 October 2008 (diff | hist) N File:DiaMed.PNG (top)
- 20:22, 28 October 2008 (diff | hist) N File:BioRad.PNG (top)
- 20:21, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 20:15, 28 October 2008 (diff | hist) N File:SNCLavalin.PNG (top)
- 20:14, 28 October 2008 (diff | hist) N File:EngFac.PNG (top)
- 20:13, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 20:11, 28 October 2008 (diff | hist) N File:ChemEng.PNG (top)
- 20:10, 28 October 2008 (diff | hist) N File:NEB.PNG (top)
- 20:09, 28 October 2008 (diff | hist) N File:FSF.PNG (top)
- 20:08, 28 October 2008 (diff | hist) N File:DeptBio.PNG (top)
- 20:08, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 20:04, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 20:03, 28 October 2008 (diff | hist) N File:SciFac.PNG (top)
- 20:03, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:58, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:46, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:43, 28 October 2008 (diff | hist) N File:MEF.PNG (top)
- 19:18, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:08, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 19:07, 28 October 2008 (diff | hist) m Team:Waterloo/Team
- 18:57, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 18:30, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 18:07, 28 October 2008 (diff | hist) N File:Adam Stinchcombe.PNG (top)
- 18:06, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 18:03, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 18:00, 28 October 2008 (diff | hist) N File:Vanja Polic.PNG (top)
- 18:00, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 17:53, 28 October 2008 (diff | hist) File:Emily Wedland.PNG (uploaded a new version of "Image:Emily Wedland.PNG": Reverted to version as of 17:18, 28 October 2008) (top)
- 17:49, 28 October 2008 (diff | hist) File:Emily Wedland.PNG (uploaded a new version of "Image:Emily Wedland.PNG")
- 17:48, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 17:18, 28 October 2008 (diff | hist) N File:Emily Wedland.PNG
- 17:17, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 14:53, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:51, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:47, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:08, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:05, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 13:53, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 13:31, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 13:09, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:35, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Making SOB Media (New page: <big><b><u>Making SOB Medium</u></b></big><br> Used in growing bacteria for preparing chemically competent cells <br> <b>Ingredients </b><br> - 0.5% (w/v) yeast extract <br> - 2% (w/v)...)
- 19:28, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Competent Cells (New page: <big><b><u>Recipe for Competent Cells </u></b></big> <br> <b>Materials </b><br> Frozen stock DH5a <br> 250mL Erlenmeyer Flask (w/ caps) <br> LB tubes <br> Sterile LB <br> <b>Instructio...)
- 19:08, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Making LB Media (New page: <big><b><u>Making LB Media</u></b></big> <br> <b>Materials </b><br> 500 mL media bottle or rack of test tubes <br> 1 L beaker <br> Magnetic stir bar <br> Tryptone <br> Yeast extract<br> ...)
- 18:59, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Making AI Media (New page: <b><u><big>Making Auto Induction (AI) Media</big></u></b><br> <b>Before you start </b><br> Certain parts are on variable copy number plasmids. The plasmid is normally low copy number, b...)
- 18:47, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Transformation (New page: <b><u><big>Transformation</big></u></b><br> <b>Before you start </b><br> Cells lose their competence once thawed, so be sure to:<br> a) keep them on ice at all times.<br> b) use cells imm...)
- 18:26, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Inoculation (New page: <big><b><u>Inoculation Protocol </u></b></big><br> <b>Materials </b><br> DNA Samples <br> Ampicillin (2µL/sample)<br> Kanamycin (2.5µL/sample)<br> Liquid LB Media (1 tube/sample)<br> <...)
- 18:20, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation (New page: <b><u><big>Glycerol Stock Preparation </big></u></b><br> <b>Before you start </b><br> - It is important to use aseptic technique when carrying out the procedures <br> <b>Materials </b><...)
- 18:10, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Ligation (New page: <big><b><u>Ligation (10µL recipe with 5 insert : 1 vector ratio)</u></b></big> <br> <b>Materials </b><br> MQ water <br> Ligase buffer<br> Ligase <br> Eppendorf tube<br> Vector DNA<br> I...)
- 18:03, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Gel Electrophoresis (New page: <b><u><big>Gel Electrophoresis</big></u></b><br> <b>Materials </b><br> Agarose <br> 1X TAE buffer <br> Gel Red<br> 1:10 diluted 1kb DNA ladder<br> Loading Dye<br> Gel Rig<br> <b>Instru...)
- 17:43, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/DNA Extraction (New page: <b><u><big>DNA Extraction </big></u></b><br> <b>Materials </b><br> All required materials provided in the Fermentas DNA Extraction Kit (K0513)<br> <b>Instructions </b><br> 1. Follow the ...)
- 17:31, 27 October 2008 (diff | hist) Team:Waterloo/Notebook (→Protocols)
- 17:31, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/DNA Digestion (New page: <b><u><big>Digestion of Plasmid DNA </big></u></b><br> <b>Before you start </b><br> - Know what is the total digestion volume mix. From that you can determine the enzyme concentration whi...)
- 17:25, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Diagnostic Digestion (New page: <b><u><big>Diagnostic Digestion of Plasmid DNA </big></u></b><br> <b>Before you start </b><br> - Know what is the total digestion volume mix. From that you can determine the enzyme concen...) (top)
- 17:02, 27 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Colony PCR
- 17:00, 27 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Colony PCR
- 16:57, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Colony PCR (New page: <b><u><big>Colony PCR </big></u></b><br> <b>Materials</b><br> - 19 µL of Master Mix<br> - 1 µL<br> <u>Calculations for Master Mix</u><br> ___ number of colonies (and control) <br> (__...)
- 15:44, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Miniprep (New page: <b><u><big>1-2-3 Miniprep (Resuspension, Lysis, Neutralization)</big></u></b><br> <b>Before you start</b><br> Prepare solutions 1, 2, and 3. Only solution 2 needs to be made fresh. Soluti...)
- 18:26, 26 October 2008 (diff | hist) N File:1Sequence (6).jpg (top)
- 18:17, 26 October 2008 (diff | hist) N File:1Sequence (4).jpg (top)
- 18:16, 26 October 2008 (diff | hist) N File:1Sequence (5).jpg (top)
- 18:15, 26 October 2008 (diff | hist) File:Sequence (5).jpg (uploaded a new version of "Image:Sequence (5).jpg") (top)
- 18:15, 26 October 2008 (diff | hist) Team:Waterloo/Project (→Steps in Assembly)
- 18:12, 26 October 2008 (diff | hist) Team:Waterloo/Project (→Steps in Assembly)
- 18:11, 26 October 2008 (diff | hist) Team:Waterloo/Project (→Steps in Assembly)
- 18:11, 26 October 2008 (diff | hist) File:Sequence (5).jpg (uploaded a new version of "Image:Sequence (5).jpg")
- 18:05, 26 October 2008 (diff | hist) Team:Waterloo/Project (→Steps in Assembly)
- 18:04, 26 October 2008 (diff | hist) File:Sequence (4).jpg (uploaded a new version of "Image:Sequence (4).jpg") (top)
- 18:04, 26 October 2008 (diff | hist) Team:Waterloo/Project (→Steps in Assembly)
- 18:03, 26 October 2008 (diff | hist) File:Sequence (4).jpg (uploaded a new version of "Image:Sequence (4).jpg": Reverted to version as of 01:32, 25 October 2008)
- 18:03, 26 October 2008 (diff | hist) File:Sequence (4).jpg (uploaded a new version of "Image:Sequence (4).jpg")
- 17:58, 26 October 2008 (diff | hist) File:Sequence (3).jpg (uploaded a new version of "Image:Sequence (3).jpg") (top)
- 17:54, 26 October 2008 (diff | hist) Team:Waterloo/Project (→Steps in Assembly)
- 17:53, 26 October 2008 (diff | hist) File:Sequence (2).jpg (uploaded a new version of "Image:Sequence (2).jpg") (top)
- 17:53, 26 October 2008 (diff | hist) File:Sequence (2).jpg (uploaded a new version of "Image:Sequence (2).jpg")
- 17:46, 26 October 2008 (diff | hist) Team:Waterloo/Project (→Steps in Assembly)
- 17:44, 26 October 2008 (diff | hist) File:Sequence (1).jpg (uploaded a new version of "Image:Sequence (1).jpg") (top)
- 03:24, 26 October 2008 (diff | hist) N File:Construct 1.jpg (top)
- 03:17, 26 October 2008 (diff | hist) N File:Construct 1.JPG (top)
- 16:05, 24 October 2008 (diff | hist) N Waterloo/23 October 2008 (New page: <u>08/10/23 Thu</u><br> [x] Kit prep rfp'2/5 pRecA3<br> [x] Nanodrop<br> [x] Send above samples for sequencing along with rTT+POCI and rPlac+TT samples from September 17th<br> [x] make m...) (top)
- 16:01, 24 October 2008 (diff | hist) N Waterloo/21 October 2008 (New page: <u>08/10/21 Tues</u><br> [x] run gel of all colony PCR results<br> [x] inoculate decent looking stuff<br> [x] bring official wiki up to date with lab activities<br>) (top)
- 15:58, 24 October 2008 (diff | hist) N Waterloo/22 October 2008 (New page: <u>08/10/22 Wed</u><br> [x] kit prep of decent looking samples from 10/21 gel<br> - inoculations for all 6 tubes of rfp'2/5 PrecA3 failed<br> [x] nanodrop kit preps<br> [x] send for seque...) (top)
- 15:53, 24 October 2008 (diff | hist) Team:Waterloo/Notebook (→Protocols)
- 15:52, 24 October 2008 (diff | hist) Team:Waterloo/Notebook (→Recipes)
- 15:50, 24 October 2008 (diff | hist) Team:Waterloo/Notebook (→Recipes)
- 15:50, 24 October 2008 (diff | hist) Team:Waterloo/Notebook (→Protocols)
- 15:28, 24 October 2008 (diff | hist) Team:Waterloo/Notebook
- 15:09, 24 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:59, 24 October 2008 (diff | hist) Team:Waterloo/Notebook
- 14:57, 24 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:56, 24 October 2008 (diff | hist) Team:Waterloo/Modeling
- 14:55, 24 October 2008 (diff | hist) Team:Waterloo/Parts
- 14:54, 24 October 2008 (diff | hist) Team:Waterloo/Project
- 14:53, 24 October 2008 (diff | hist) Team:Waterloo/Team
- 14:53, 24 October 2008 (diff | hist) Team:Waterloo/Notebook
- 14:36, 24 October 2008 (diff | hist) N Team:Waterloo/Sponsors (New page: ==2008 Sponsors== ===Gold Level=== <html><center><img src="http://igem.uwaterloo.ca/share/Logos/MEFLogo.png" width="453px" height="147px"><br> <a href="http://www.mef.uwaterloo.ca/"> Mathe...)
- 14:33, 24 October 2008 (diff | hist) Team:Waterloo
- 15:45, 21 October 2008 (diff | hist) N Waterloo/20 October 2008 (New page: <u>08/10/20 Monday</u><br> [x] patches of transformants<br> [x] Stuff tips and general housekeeping<br> [x] Autoclave<br> [x] colony PCR of patches from morning<br>) (top)
- 15:41, 21 October 2008 (diff | hist) N Waterloo/19 October 2008 (New page: <u>08/10/19 Sunday</u><br> [x] transformation of ligations<br>) (top)
- 15:37, 21 October 2008 (diff | hist) N Waterloo/18 October 2008 (New page: <u>08/10/18 Saturday</u><br> [] kit prep Plac-rfp'<br> - Inoculations seemed to have failed again<br> [x] digest (digest 16 ul of a ~600-800 bp part in pSB1A2 so we can subclone into this...) (top)
- 15:33, 21 October 2008 (diff | hist) N Waterloo/17 October 2008 (New page: <u>08/10/17 Friday</u><br> [x] kit prep of transformant inoculants<br> - also, kit prep of plac-rfp'<br> - Inoculations for Plac-rfp' from frozen stock did not work<br> [] digest (digest...) (top)
- 15:27, 21 October 2008 (diff | hist) N Waterloo/16 October 2008 (New page: <u>08/10/16 Thursday</u><br> [x] inoculate transformants (use one colony from each, but inoculate in duplicate!)<br> [x] BIOMORTAR<br> [x] update Box Contents Google Doc with location nu...) (top)
- 15:25, 21 October 2008 (diff | hist) N Waterloo/15 October 2008 (New page: <u>08/10/15 Wednesday</u><br> [x] transform c0051' 6, c0051''-4, PMEI gene 6 (1,2,4)<br>) (top)
- 15:22, 21 October 2008 (diff | hist) N Waterloo/14 October 2008 (New page: <u>08/10/14 Tuesday</u> <br> [] send stuff for sequencing<br> [] inoculate stuff to kit prep more DNA of (running low on some of the stuff we need to subclone/assemble)<br> - c0051', c00...) (top)
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