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...oree? See the <a class="pink" href="https://2008.igem.org/Jamboree/Media">Media</a> page for pictures and videos. Post your own links to let us know where **[[Jamboree/Media | Media ]](pictures & fun videos!)

10 KB (1,324 words) - 20:30, 5 May 2016
Team:Illinois/Antibody GPCR Fusion
...of the FUS1-Fluo.Prot. fusion into the yeast genome, which seems to be the standard for this type of thing. *Preparation of Yeast Media [http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/article/m

21 KB (3,227 words) - 02:19, 24 October 2008
Team:Hawaii/Project/Part B
...A'' and ''slr2016'' secretion signal sequences will be syntheized with the standard Biobrick sites. Oligonucleotide fragments of each will be hybridized with i ...efficacy of the signal peptides in transporting GFP into the extracellular media can be measured using a spectrofluorometer.

11 KB (1,558 words) - 02:25, 29 October 2008
Team:University of Chicago/Notebook/Norayucel
7. Added 250microliters SOC media (prepared at 1:30) ...0microliters for pGreen, 1mL for E0240 (scaled up to accommodate for extra media dilution)

11 KB (1,571 words) - 16:42, 28 September 2008
Team:EPF-Lausanne/Notebook
===Media and buffers=== ..., animals or the environment, other than the ones which are encountered in standard laboratory work with ''E.coli''. Therefore, we estimate the risk to researc

16 KB (2,322 words) - 23:27, 29 October 2008
Team:Heidelberg/Human Practice/Open Day
...translated the text in english, the original german version can be found [[Media:IGEM-Veranstaltung_Heidelberg.pdf|'''here''']]). ...en_Day_english.ppt.html here] (rapdishare download file) and as pdf-file [[Media:Open_Day_english.pdf| here]]. 

60 KB (9,576 words) - 15:58, 29 October 2008
Team:UCSF/Materials and Methods
...gal2::NatR, allowing graded activation of galactose inducible promoters ([[Media:Hawkins and Smolke.pdf]]). ...ere grown to early log phase (OD600=0.1-0.3) in complete synthetic dropout media and then treated with 1 μM α-factor (Zymo Research) to activate the pathw

3 KB (499 words) - 03:27, 30 October 2008
Team:Alberta NINT/Project
[[Media:NINT_PlasmidDesignAndConstruction.pdf|Plasmid Design and Construction]] ...ion in the absence of any input. The y-axis is shown in Miller units (the standard for such assays).

21 KB (3,119 words) - 16:44, 30 March 2009
Team:Hawaii/Member Training
## Read [[Image:Idempotent Vector Design for Standard Assembly of Biobricks.pdf|Biobrick Technical Manual]], don't worry about un ## [http://igem.blip.tv/file/254379 Drew Endy: What is a Standard Biological Part] 2 minute 31 seconds

2 KB (309 words) - 12:16, 31 May 2008
Team:UC Berkeley/Protocols
# Plate using the sterilized glass-bead method or with standard spreaders. # Plate using the sterilized glass-bead method or with standard spreaders.

17 KB (2,857 words) - 22:03, 27 October 2008
Jamboree/Compete
...mboree/Compete#Requests_for_variance">Submit any requests for non-BioBrick standard methods</a> : October 8</li> ...xpected to submit their parts to the [http://partsregistry.org Registry of Standard Biological Parts]. In addition to submitting your parts, you must also dec

13 KB (1,920 words) - 06:14, 22 April 2016
Team:Waterloo/Notebook
...plication written in-house for administration of BioBricks. It understands standard assembly and can gather information from the Parts Registry. BioMortar will ...hat it can construct sequences for composite parts automatically. Although Standard Assembly makes it easy to ligate parts, it is fairly easy to reverse the en

3 KB (456 words) - 04:05, 30 October 2008
SOC
#Follow directions to make 1 liter of [[SOB]] media [[Category:Material]] [[Category:Standard Media]]

546 B (73 words) - 02:24, 30 May 2008
Team:Waterloo/Project
...nucleases during uninduced conditions. Cultures will initially be grown on media containing IPTG. Expression of the unstable cI repressor (C0051) will be dr ...me degradation construct will be integrated into the genome via a proposed standard integration vector.

11 KB (1,711 words) - 04:00, 30 October 2008
Team:Groningen/Notebook
...e [http://partsregistry.org/Assembly:Standard_assembly Standard Assembly from the parts registry] site. When we had to assembl '''Media'''

4 KB (643 words) - 12:10, 16 September 2008
Alberta NINT/23 July 2008
showed sufficient cell growth after two days at 37 C. Prepared media (LB+antibiotics+IPTG+glucose/arabinose) and inoculated 1 ml of media with 20 ul of cell cultures

968 B (108 words) - 23:02, 11 October 2008
Team:Davidson-Missouri Western
...rts. We also produced a graphical interface for exploring the Registry of Standard Biological Parts called Viz-A-Brick, and other web-based tools to improve t i'''GEM 2008 powerpoint presentation''' [[Media:MWSU_Davidson_iGEM08.PPT]]

4 KB (583 words) - 03:55, 30 October 2008
Team:University of Washington/Measurement Kit
... After 16 hours, each overnight culture was diluted 100 fold into fresh M9 media with kanamycin, and grown for 3 hours. After three hours, two 200uL samples

4 KB (577 words) - 04:09, 30 October 2008
Team:Peking University/Project
...s yeast codon optimized kindly offered by Prof. Youri I Pavlov. A set of [[Media:Linkers.pdf|12 linkers]] were synthesized to test the ability to maintain t ...flanking the 3’ of the promoter. Other constructs were accomplished with standard protocols.

18 KB (2,815 words) - 05:26, 30 October 2008
Team:Heidelberg/Notebook/Killing II/5thweek
*Retransformation of ligation into TOP 10 -> standard protocoll *Preparation of M9 media

27 KB (3,683 words) - 21:27, 28 October 2008
Brown: Team Resistance/14 July 2008
*Lyse cells in minimal media instead of LB because the ion content of minimal media is controlled and known *use standard copper wire in the breadboard for the circuit and only use gold/platinum fo

5 KB (771 words) - 18:58, 29 October 2008
Teachtheteachers
10:00 am -- Jason Kelly -- Standard Measurement Kit for BioBrick Promoters (PoPS-out devices) ...xplains how to measure the PoPs of promoters and compare it to a reference standard.

5 KB (778 words) - 20:34, 8 May 2008
Team:ETH Zurich/Project/Motivation
...ard E. coli, the cells with reduced genome grew slightly denser in certain media, tolerated a greater variety of plasmids and had higher electroporation eff

6 KB (907 words) - 09:57, 30 October 2008
Team:Caltech/Protocols/Measuring H2O2
...(100uM, 33.3uM, 11.1 uM, 3.7 uM and 0.0 uM H2O2) made in the corresponding media. [[Image:H2O2 standards example.png|thumb|center|400px|An example of a typical standard curve generated by the assay. Error bars (1SD, n=3) are too small to be see

1 KB (162 words) - 00:24, 30 October 2008
SOB
[[Category:Material]] [[Category:Standard Media]]

1 KB (171 words) - 02:13, 31 May 2008
Team:Illinois/Antibody RTK Fusion
...pplies needed will include the cholera B subunit, yeast culture media, and standard molecular biology reagents/kits.

7 KB (1,026 words) - 04:36, 30 October 2008
Team:Freiburg/Project
...he construction of fusion proteins due to extended Biobrick 3.0 (Freiburg) standard. Cloning was done in E. coli. However, we test our receptors in huma ...Origami-yield and measurement of the molecule´s stability in cell culture media the later ones. 

12 KB (1,733 words) - 08:24, 30 October 2008
Team:Illinois/Antibody GPCR Fusion Notebook
** Brought to standard concentration, 30uM *Add SOC Media (200uL)

13 KB (1,969 words) - 02:55, 30 October 2008
Team:TUDelft/Ethics conclusions
'''Full report: Click this [[Media:TUDelft_iGEM_Ethics_StevenF.pdf|link]]''' ... involved and hence the good products will not be added to the registry of standard biological parts.

20 KB (3,152 words) - 19:44, 29 October 2008
Team:Chiba/Sender experiments
#Inoculated them independently in liquid media. Incubated at 37°C 12h ...ts are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synth

6 KB (641 words) - 12:14, 29 October 2008
Team:Brown/Notebook/Timeline
...d, our apparatus became more complex. We quickly made the transition from standard voltmeter to a Data Acquisition Card coupled with LabView Computer Software *We also experimented with LB Lennox, Luria, M9 Minimal Media, Distilled/Sterile Water, as well as Milli-Q water. Cells were pelleted an

6 KB (917 words) - 17:35, 29 October 2008
Team:Heidelberg/Notebook/Killing I/Notebook/week12
* the insert was ligated in the lambda backbone over night using standard ligation methods * colonies were picked and transferred in liquid media containing chloramphenicol

36 KB (4,978 words) - 14:47, 29 October 2008
Team:Heidelberg/Human Practice/Phips the Phage/Technical Backround
...transformed bacteria to survive, if antibiotics are present in the culture media. All untransformed cells die due to the added antibiotics, because they do ...itcal introduction, the pupils carry out a plasmid minipreparation using a standard kit.

51 KB (8,118 words) - 16:56, 29 October 2008
Team:Imperial College/B.subtilis
... subtilis chassis page</a></html> on the '''Registry of Biological Standard Parts'''. We characterised the growth rate of ''B. subtilis'' in LB media at 37oC. This curve provides useful information when inducing pr

3 KB (492 words) - 03:41, 30 October 2008
Team:Slovenia/Notebook/Methods
...r the overexpression of flagellar proteins. Cultures were grown in LB media (Luria-Bertani) supplemented with ampicillin at 37°C with shaking ...dividually numbered by ear-notching, regularly examined, weighed and fed a standard rodent chow (Altromin 1324, Lage, Germany). Food was available ad libitu

48 KB (6,976 words) - 04:42, 30 October 2008
Team:Davidson-Missouri Western/Viz-A-Brick: A New Way to Visualize the Registry
As the Registry of Standard Biological Parts expands, it becomes increasingly important that users be a [[Media:Example.ogg]]

3 KB (484 words) - 18:13, 29 October 2008
Team:Chiba/Project/Experiments:Crosstalk
...ts are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synth #Inoculated them independently in liquid media. Incubated at 37°C 12h

7 KB (579 words) - 12:43, 29 October 2008
Team:Cambridge/Voltage/Lab Work
...increase volume in preparation for experiments tomorrow on K+ tolerance in media. * Designed standard infusion primers for biobrick assembly. Abandoned due to unfeasibly large s

21 KB (3,118 words) - 23:31, 29 October 2008
Team:Chiba/Project/Experiments:Sender Crosstalk
#Inoculated them independently in liquid media. Incubated at 37°C for 12hours. ...ts are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI, RhlI, and LasI means fluorescence induced by AHLs

7 KB (1,039 words) - 11:00, 30 October 2008
Team:Groningen/references.html
...ttp://openwetware.org/wiki/Griffitts:Common_media">Openwetware.org: Common Media</a></li> ...tp://partsregistry.org/Assembly:Standard_assembly">Partsregistry.org Help: Standard Assembly</a></li>

7 KB (924 words) - 00:22, 30 October 2008