Team:Chiba/Calendar-Home/6 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
(→Team:Communication)
Line 72: Line 72:
<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>6</td></tr>
+
<td>6μl</td></tr>
</table>
</table>
:From left;<br>
:From left;<br>
Line 96: Line 96:
<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>6</td>
+
<td>6μl</td>
</tr>
</tr>
</table>
</table>
Line 115: Line 115:
</tr>
</tr>
<tr>
<tr>
-
<td>PstⅠ</td>
+
<td>PstI</td>
<td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td>
<td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SpeⅠ</td>
+
<td>SpeI</td>
<td>0.5</td><td>-</td><td>1</td><td>-</td><td>-</td>
<td>0.5</td><td>-</td><td>1</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>XbaⅠ</td>
+
<td>XbaI</td>
<td>-</td><td>0.5</td><td>-</td><td>0.5</td><td>0.5</td>
<td>-</td><td>0.5</td><td>-</td><td>0.5</td><td>0.5</td>
</tr>
</tr>
Line 148: Line 148:
<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>20</td><td>60</td><td>30</td><td>60</td><td>60</td>
+
<td>20μl</td><td>60μl</td><td>30μl</td><td>60μl</td><td>60μl</td>
</tr>
</tr>
</table>
</table>

Revision as of 21:09, 29 October 2008

>Home | Notebook

5 September 2008 <|> 7 September 2008

Contents

Meeting with UmeG

the ppt (in Japanese) for our weekly meeting

  1. File:Chiba-0809060855.ppt


Laboratory work

Team:Input

no work

Team:Communication

Colony-PCR

PCR of BBa_I9030 and two control,BBa_F2620(2007)).
DNA Template 1
dNTP mix 5
Foward Primer 5
Reverse Primer 5
DNA polymerase 1
Thermopol Buffer 5
dH2O 33
TOTAL 50μL


95°C,5min -> ( 95°C,1min -> 54°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C store


--->Gel Check
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μl
From left;
1 BBa_I9030 without LVA -> OK
2 BBa_I9030 Positive Control -> OK
3 BBa_F2620 Positive control -> OK

Gel Check Sample DNA : BBa_R0010

Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μl



Digestion Test

Sample DNA:

1:BBa_R0010,2:BBa_S03154,3:BBa_J04500,4:BBa_C0161,5:BBa_C0261

Sample No. 12345
Sample DNA 11111
PstI 0.50.50.50.50.5
SpeI 0.5-1--
XbaI -0.5-0.50.5
Buffer 2 2-3--
Buffer 3 -6-66
(10×)BSA 20.63--
(100×)BSA ---0.60.6
dH2O 36.471.41.4
TOTAL 20μl60μl30μl60μl60μl

Team:Output

no work

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