Team:Chiba/Calendar-Home/6 September 2008

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5 September 2008 <|> 7 September 2008

Contents

Meeting with UmeG

the ppt (in Japanese) for our weekly meeting

  1. File:Chiba-0809060855.ppt


Laboratory work

Team:Input

no work

Team:Communication

Colony-PCR

PCR of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] and two control,[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).
DNA Template 1
dNTP mix 5
Foward Primer 5
Reverse Primer 5
DNA polymerase 1
Thermopol Buffer 5
dH2O 33
TOTAL 50μL


95°C,5min -> ( 95°C,1min -> 54°C,1min -> 72°C,1min )・・・25cycles -> 72°C,10min -> 6°C store


--->Gel Check
Chiba-0906.JPG
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μl
From left;
1 [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] without LVA -> OK
2 [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] Positive Control -> OK
3 [http://partsregistry.org/Part:BBa_F2620 BBa_F2620] Positive control -> OK

Gel Check Sample DNA : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]

Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μl



Digestion Test

Sample DNA:

1:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010],2:[http://partsregistry.org/Part:BBa_S03154 BBa_S03154],3:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500],4:[http://partsregistry.org/Part:BBa_C0161 BBa_C0161],5:[http://partsregistry.org/Part:BBa_C0261 BBa_C0261]

Sample No. 12345
Sample DNA 11111
PstI 0.50.50.50.50.5
SpeI 0.5-1--
XbaI -0.5-0.50.5
Buffer 2 2-3--
Buffer 3 -6-66
(10×)BSA 20.63--
(100×)BSA ---0.60.6
dH2O 36.471.41.4
TOTAL 20μl60μl30μl60μl60μl

Team:Output

no work