Team:Chiba/Calendar-Home/7 September 2008

From 2008.igem.org

(Difference between revisions)
(Laboratory work)
(Laboratory work)
Line 14: Line 14:
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>1,2</td>
<td>1,2</td>
</tr>
</tr>
Line 69: Line 69:
</table>
</table>
:<br>
:<br>
-
:Left side:BBa_R0010 Sample No.1
+
:Left side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.1
-
:Right side:BBa_R0010 Sample No.2
+
:Right side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.2
-->(8/9)
-->(8/9)
Line 128: Line 128:
</table>
</table>
-
-->37℃ 3hour
+
-->left at for 3 hours at 37 degrees.
-->[[Team:Chiba/protocol/ligation/gelex|Gel Extraction]]
-->[[Team:Chiba/protocol/ligation/gelex|Gel Extraction]]

Revision as of 02:06, 25 October 2008

>Home | Notebook

6 September 2008 <|> 8 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

-->(6/9) Digestion Test

Sample DNA 1 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](unknown)
Sample DNA 2 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](100ng/μL)
Sample No. 1,2
Sample DNA(μL) 12
PstⅠ(μL) 0.5
SpeⅠ(μL) 0.5
Buffer 2(μL) 2
(×10)BSA(μL) 2
dH2O(μL) 3
TOTAL(μL) 20


--->Gel Check
Sample leftright
Sample DNA(μL) 55
Loading Dye(μL) 11
TOTAL(μL) 66

Left side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.1
Right side:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] Sample No.2

-->(8/9)


Team:Output

Cloning Digestion

  • vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]① 75ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]② 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]③ 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160]④ 38.4ng/μl
  • vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤ 100ng/μl
  • insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
Sample No.
DNA(digested sample)(μL) 505454451454
SpeⅠ(μL) 100010
PstⅠ(μL) 0.50.50.50.50.50.5
XbaⅠ(μL) 00.50.50.500.5
BSA(x10)(μL) 0.677627
Buffer2(μL) 6---2-
Buffer3(μL) -776-7
dH2O(μL) 1.91120.51
TOTAL(μL) 60μl70μl70μl60μl20μl70μl

-->left at for 3 hours at 37 degrees.

-->Gel Extraction

Sample No.
DNA(digested sample)(μL) 6101010410
Loading Dye(μL) 222212
dH2O(μL) 4---12
TOTAL(μL) 12121212612
well 1077657

-->DNA purification (①-④,⑥:7μl ⑤:6μl)