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Team:Chiba/Calendar-Home/7 September 2008
From 2008.igem.org
(Difference between revisions)
(→Team:Communication) |
(→Team:Output) |
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<big>Cloning</big> | <big>Cloning</big> | ||
[[Team:Chiba/protocol/digestion|Digestion]] | [[Team:Chiba/protocol/digestion|Digestion]] | ||
| - | *vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | + | *vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)75ng/μl |
| - | *insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158] | + | *insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl |
| - | *insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156] | + | *insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl |
| - | *insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160] | + | *insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](4) 38.4ng/μl |
| - | *vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | + | *vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl |
| - | *insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008] | + | *insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl |
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<tr> | <tr> | ||
<td width="257">Sample No.</td> | <td width="257">Sample No.</td> | ||
| - | <td> | + | <td>1</td><td>2</td><td>3</td><td>4</td><td></td><td>6</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 94: | Line 94: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>SpeI(μL)</td> |
<td>1</td><td>0</td><td>0</td><td>0</td><td>1</td><td>0</td> | <td>1</td><td>0</td><td>0</td><td>0</td><td>1</td><td>0</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>PstI(μL)</td> |
<td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td> | <td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0.5</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>XbaI(μL)</td> |
<td>0</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0</td><td>0.5</td> | <td>0</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0</td><td>0.5</td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td width="257">Sample No.</td> | <td width="257">Sample No.</td> | ||
| - | <td> | + | <td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 157: | Line 157: | ||
</table> | </table> | ||
| - | -->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]] ( | + | -->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]] (1~4,6:7μl 5:6μl) |
Revision as of 22:08, 29 October 2008
6 September 2008 <|> 8 September 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
-->(6/9) Digestion Test
| Sample No. | 1,2 |
| Sample DNA(μL) | 12 |
| PstI(μL) | 0.5 |
| SpeI(μL) | 0.5 |
| Buffer 2(μL) | 2 |
| (×10)BSA(μL) | 2 |
| dH2O(μL) | 3 |
| TOTAL(μL) | 20 |
- --->Gel Check
Sample left right Sample DNA(μL) 5 5 Loading Dye(μL) 1 1 TOTAL(μL) 6 6 -->(8/9)
Team:Output
Cloning Digestion
- vector:BBa_R0040(1)75ng/μl
- insert:BBa_S03158(2) 33.6ng/μl
- insert:BBa_S03156(3) 33.6ng/μl
- insert:Ba_S03160(4) 38.4ng/μl
- vector:BBa_R0010(5) 100ng/μl
- insert:BBa_J52008(6) 33.6ng/μl
Sample No. 1 2 3 4 6 DNA(digested sample)(μL) 50 54 54 45 14 54 SpeI(μL) 1 0 0 0 1 0 PstI(μL) 0.5 0.5 0.5 0.5 0.5 0.5 XbaI(μL) 0 0.5 0.5 0.5 0 0.5 BSA(x10)(μL) 0.6 7 7 6 2 7 Buffer2(μL) 6 - - - 2 - Buffer3(μL) - 7 7 6 - 7 dH2O(μL) 1.9 1 1 2 0.5 1 TOTAL(μL) 60μl 70μl 70μl 60μl 20μl 70μl -->left at for 3 hours at 37 degrees.
Sample No. 1 2 3 4 5 6 DNA(digested sample)(μL) 6 10 10 10 4 10 Loading Dye(μL) 2 2 2 2 1 2 dH2O(μL) 4 - - - 1 2 TOTAL(μL) 12 12 12 12 6 12 well 10 7 7 6 5 7 -->DNA purification (1~4,6:7μl 5:6μl)
