Team:Hawaii/Notebook/2008-09-30

From 2008.igem.org

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(margaret)
(Construction of secretion device (cont.))
 
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::* rbs+GFPf+tt #1, 2 (yet to be confirmed via sequencing)
::* rbs+GFPf+tt #1, 2 (yet to be confirmed via sequencing)
::* GFPf+tt #21
::* GFPf+tt #21
-
:* RE digest:
+
:* Overnight RE digest:
::*EcoRI/PstI:
::*EcoRI/PstI:
:::* pSB1A3
:::* pSB1A3
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:::* nir+rbs
:::* nir+rbs
:::* plac+rbs
:::* plac+rbs
 +
:::* slr1+GFPf
:::* nir+rbs+pilA #17  
:::* nir+rbs+pilA #17  
::* XbaI/PstI:
::* XbaI/PstI:
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:::* rbs+GFPf+tt #1, 2
:::* rbs+GFPf+tt #1, 2
:::* GFPf+tt #21
:::* GFPf+tt #21
 +
:::* rbs+GFP
===Plasmid Prep===
===Plasmid Prep===
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:*pSB1A3, plac/rbs/rep + pSB1A3 (also made a glycerol stock of this), and oriT in pSB1A3
:*pSB1A3, plac/rbs/rep + pSB1A3 (also made a glycerol stock of this), and oriT in pSB1A3
-
===Re-digest from yesterday===
+
===RE-digest from yesterday===
:<strong> Margaret </strong>
:<strong> Margaret </strong>
[[Image:gel_purification_9-30.jpg|right|thumb|300px|Gel purification of re-digest of aadaBB, aadapRL, and oriV.]]
[[Image:gel_purification_9-30.jpg|right|thumb|300px|Gel purification of re-digest of aadaBB, aadapRL, and oriV.]]

Latest revision as of 23:43, 30 September 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Plasmid Prep

Grace
  • Plasmid prep of BBpRL1383a #1, 3, 13, 18

Construction of secretion device (cont.)

Grace
  • PCR:
  • nir+rbs
  • slr1+GFPf
  • plac+rbs
  • rbs+GFP
  • nir
  • nir+rbs+pilA #17 (yet to be confirmed via sequencing)
  • rbs+GFPf+tt #1, 2 (yet to be confirmed via sequencing)
  • GFPf+tt #21
  • Overnight RE digest:
  • EcoRI/PstI:
  • pSB1A3
  • EcoRI/SpeI:
  • nir
  • rbs+GFP
  • nir+rbs
  • plac+rbs
  • slr1+GFPf
  • nir+rbs+pilA #17
  • XbaI/PstI:
  • slr1+GFPf
  • rbs+GFPf+tt #1, 2
  • GFPf+tt #21
  • rbs+GFP

Plasmid Prep

Margaret
  • pSB1A3, plac/rbs/rep + pSB1A3 (also made a glycerol stock of this), and oriT in pSB1A3

RE-digest from yesterday

Margaret
File:Gel purification 9-30.jpg
Gel purification of re-digest of aadaBB, aadapRL, and oriV.
  • gel purification of aadaBB, aadApRL, and oriV
  • de-phosphorylate pSB1A2, pSB1A3a, pSB3K3

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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