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Team:KULeuven/22 August 2008
From 2008.igem.org
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=== Wet Lab === | === Wet Lab === | ||
| + | * We made digests (with ''Eco''RI and ''Spe''I) of the following ligations: R0084+J23022+J23109+J23032, R0084+B0032, R0040+J23022 and K145001. After that, these digests were put on gel. They seemed to have been cut properly, but when we purified these parts from the gel the concentrations were very low. | ||
| + | * We also did some PCRs to construct BioBrick K145014 (T7 polymerase with UmuD tag) and to test the transduction. | ||
| + | * We started a few ligations (the ones that failed before): C0040+B0015, K145015+B0015, C0012+B0015, C0060+B0015, K145001+B0015 and R0040+J23022. | ||
=== Dry Lab === | === Dry Lab === | ||
Revision as of 12:36, 24 August 2008
dock/undock dropdown
Contents |
Lab Work
Wet Lab
- We made digests (with EcoRI and SpeI) of the following ligations: R0084+J23022+J23109+J23032, R0084+B0032, R0040+J23022 and K145001. After that, these digests were put on gel. They seemed to have been cut properly, but when we purified these parts from the gel the concentrations were very low.
- We also did some PCRs to construct BioBrick K145014 (T7 polymerase with UmuD tag) and to test the transduction.
- We started a few ligations (the ones that failed before): C0040+B0015, K145015+B0015, C0012+B0015, C0060+B0015, K145001+B0015 and R0040+J23022.
Dry Lab
Modeling
Wiki
Official super duper release of wiki homepage and global style, but needs fixes to match with annoying IE browser. Picture gallery fixes will have to wait.
Remarks
Tippenvulwedstrijd: Hanne: 1 min 29! Getting better!
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