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Team:KULeuven/30 August 2008
From 2008.igem.org
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A glycerol stock was made of the new parts and of our own parts ([http://partsregistry.org/Part:BBa_K145151 K145151], [http://partsregistry.org/Part:BBa_K145001 K145001], [http://partsregistry.org/Part:BBa_K145015 K145015]). We forgot one, so a liquid culture was made of part [http://partsregistry.org/Part:BBa_R0053 R0053]. | A glycerol stock was made of the new parts and of our own parts ([http://partsregistry.org/Part:BBa_K145151 K145151], [http://partsregistry.org/Part:BBa_K145001 K145001], [http://partsregistry.org/Part:BBa_K145015 K145015]). We forgot one, so a liquid culture was made of part [http://partsregistry.org/Part:BBa_R0053 R0053]. | ||
| - | We miniprepped the new parts and the two ligations that succeeded ([http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015] that were plated on Ap instead of Km). | + | We miniprepped the new parts and the two ligations that succeeded ([http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015] that were plated on Ap instead of Km). They were then cut with ''Xba''I. |
The digests with ''Xba''I were started: [http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_B0033 B0033], [http://partsregistry.org/Part:BBa_B0034 B0034], [http://partsregistry.org/Part:BBa_J23066 J23066], [http://partsregistry.org/Part:BBa_P0353 P0353], [http://partsregistry.org/Part:BBa_P0152 P0152], [http://partsregistry.org/Part:BBa_E0240 E0240]. | The digests with ''Xba''I were started: [http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_B0033 B0033], [http://partsregistry.org/Part:BBa_B0034 B0034], [http://partsregistry.org/Part:BBa_J23066 J23066], [http://partsregistry.org/Part:BBa_P0353 P0353], [http://partsregistry.org/Part:BBa_P0152 P0152], [http://partsregistry.org/Part:BBa_E0240 E0240]. | ||
Latest revision as of 15:30, 12 October 2008
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Contents |
Lab Work
Wet Lab
A glycerol stock was made of the new parts and of our own parts (K145151, K145001, K145015). We forgot one, so a liquid culture was made of part R0053.
We miniprepped the new parts and the two ligations that succeeded (C0040+B0015 and C0060+B0015 that were plated on Ap instead of Km). They were then cut with XbaI.
The digests with XbaI were started: B0015, B0033, B0034, J23066, P0353, P0152, E0240.
The ligations we made yesterday were electroporated today (still in electrocompetent Top10 cells): R0011+B0032, C0040+B0015, C0060+B0015, C0012+B0015, K145015+B0015, K145001+B0015, K145151+B0015 and (R0040+J23022)+(J23109+J23032).
Dry Lab
General
Created the oligos for the B1002 and B1006 terminators link
Modeling
Final updated model uploaded to the wiki (only MatLab)
Remarks
I don't think the dam methylation is the problem with our ligations. This is the prefix: gaattcgcggccgcttctagag. The recognition site of XbaI is in bold. There is no overlap for dam methylation (gatc).
