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Team:KULeuven/30 August 2008
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Revision as of 19:40, 30 August 2008 by Elkevanassche (Talk | contribs)
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Contents |
Lab Work
Wet Lab
- A glycerol stock was made of the new parts.
- We MiniPrepped the new parts and the two ligations that succeeded (C0040+B0015 and C0060+B0015).
- The digestions with XbaI were started.
- The ligations we made yesterday were electroporated today (still in electrocompetent Top10 cells).
- A liquid culture was made of part R0053.
Dry Lab
Created the oligos for the B1002 and B1006 terminators [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=terminator link]
Modeling
Final updated model uploaded to the wiki (only MatLab)
Wiki
Remarks
I don't think the dam methylation is the problem with our ligations. This is the prefix: gaattcgcggccgcttctagag. The recognition site of XbaI is in bold. There is no overlap for dam methylation (gatc).
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