Team:KULeuven/30 August 2008

From 2008.igem.org

• Home
• Reports
• Calendar

• Project abstract
• Meet the team
• Leave a message
• Mail us

• The Team
  Research Labs and Groups Students Instructors Advisors Pictures
• The Project
  Summary Components

  Input Output Filter InverTimer Reset Cell Death Memory

  Safety End Evaluation Literature Brainstorm
• Ethics
  Introduction Three A's

  Defining the three A's Developments in modern physics Developments in biology

  Concerns & Issues

  Defining synthetic biology A framework Concerns Issues The need for answers

  Biological Robotics

  KULeuven iGEM 2008 project Biological robotics

  Afterword References
• Submitted Parts
  Sandbox
• Modeling
  Overview Kinetic Constants Components

  Output Filter InverTimer Reset Cell Death Memory

  Full Model Sensitivity Analysis Multi-cell Model Diffusion
• Data Analysis
  Overview New Parts

  GFP (LVA-tag) Other

  Components

  Input Output Other
• Software
  Multi-cell Toolbox Simbiology2LaTeX Toolbox
• Notebook
  Protocols Reports

  Daily Weekly

  Lab Data

  Freezer Primers Ligation

  Tools Press Acknowledgments

Lab Work

Wet Lab

• A glycerol stock was made of the new parts and of our own parts (K145151, K145001 and K145015).
• We MiniPrepped the new parts and the two ligations that succeeded (C0040+B0015 and C0060+B0015).
• The digestions with XbaI were started.
• The ligations we made yesterday were electroporated today (still in electrocompetent Top10 cells).
• A liquid culture was made of part R0053.

Dry Lab

Created the oligos for the B1002 and B1006 terminators link

Modeling

Final updated model uploaded to the wiki (only MatLab)

Wiki

Remarks

I don't think the dam methylation is the problem with our ligations. This is the prefix: gaattcgcggccgcttctagag. The recognition site of XbaI is in bold. There is no overlap for dam methylation (gatc).

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Printable version
• Permanent link
• Privacy policy
• Disclaimers