Team:UNIPV-Pavia/Notebook/Week14

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Notebook



Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7
Week 8 Week 9 Week 10 Week 11 Week 12 Week 13 Week 14
Week 15 Week 16 Week 17 Week 18 Week 19 Week 20 Week 21



Week 11: 08/21/08 - 08/22/08

08/21/08

  • Sequencing results:
    • 27 - wrong sequence
    • BBa_C0062 - OK
    • 12 - OK
    • 22 - point mutation C->T
    • 30 - point mutation C->T
  • We decided to re-perform 34 and 35 assembly from 12, whose sequence was correct.
  • We prepared 0.5 l of LB + Amp for plates.
  • We infected 9 ml LB + Amp with 30 µl of b and 12 glycerol stocks.

08/22/08

  • Glycerol stocks/miniprep for b and 12.
  • We planned experiments for qualitative tests.