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Team:UNIPV-Pavia/Notebook/Week15
From 2008.igem.org
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**12 (E-S) - BBa_B1006 (E-X) (to re-perform mutated assemblies) | **12 (E-S) - BBa_B1006 (E-X) (to re-perform mutated assemblies) | ||
*We incubated ligations at 16°C overnight. | *We incubated ligations at 16°C overnight. | ||
| + | |||
| + | *We ordered 3OC6HSL (Sigma). | ||
'''08/26/08''' | '''08/26/08''' | ||
Revision as of 20:45, 23 September 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
Notebook
| Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
|---|---|---|---|---|---|---|
| Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 |
| Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
Week 15: 08/25/08 - 08/29/08
08/25/08
- Pladmid digestion for:
- BBa_R0051 (S-P)
- BBa_R0040 (S-P)
- b (E-X)
- BBa_B1006 (E-X)
- 12 (E-S)
- Run/gel extraction.
- Ligations:
- BBa_R0051 (S-P) - BBa_E0240 (X-P) (for promoter test)
- BBa_R0040 (S-P) - BBa_E0240 (X-P) (for promoter test)
- 12 (E-S) - b (E-X) (for AND logic gate test)
- 12 (E-S) - BBa_B1006 (E-X) (to re-perform mutated assemblies)
- We incubated ligations at 16°C overnight.
- We ordered 3OC6HSL (Sigma).
08/26/08
- We transformed/plated ligations.
08/27/08
- Plates grew correctly. We checked colony fluorescence of three plates under UV rays:
- BBa_R0051-BBa_E0240 glowed
- BBa_R0040-BBa_E0240 glowed
- 12-b (BBa_R0051-BBa_B0030-BBa_C0062-BBa_B0030-BBa_C0061-BBa_B1006-BBa_R0062-BBa_E0240) glowed
- Colony PCR for 5 colonies of 12-BBa_B1006 (called 22).
- Gel results: we chose 1st colony to grow a 9 ml overnight culture.
08/28/08
- Glycerol stocks/miniprep for 22.
- We sent purified plasmids to Primm for sequencing.
- We infected 9 ml of LB + Amp with 30 µl of BBa_R0062 glycerol stock.
08/29/08
- Glycerol stock/miniprep for BBa_R0062.
