From 2008.igem.org
Things we did today
Wetlab Work
Practiced getting DNA plasmids off the filter paper
- Norman and Krystle
- see experiment for details
- DNA will be frozen in TE for later transformation
- BBa_E0040 GFP biobrick (spot 7E on plate 1001)
- BBa_J04430 IPTG-induced GFP device (spot 4H on plate 1004)
- BBa_I13522 constitutive GFP device (spot 8A on plate 1002)
Made Spectinomycin/Streptomycin Plates
- Grace
- Made 1000X sp stock solution
- Made 1000X sm stock solution
- Made BG-11+sp+sm plates
- Made LB+sp+sm plates
- Made LB+sp plates
Transformed BioBricks into competent DH5α
- Grace
Drylab Work
Discussion
Quote of the Day
"So that's the difference between biochemists and geneticists...
- Biochemists will be so meticulous and do everything the long way.
- Geneticists will try to look for a shortest, simplest possible."
[http://manoa.hawaii.edu/
][http://manoa.hawaii.edu/ovcrge/
][http://www.ctahr.hawaii.edu
]
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