Revision as of 18:03, 20 October 2008

Last number used

Miniprep	Strain	Ligation	Digestion	PCR
216	216	213	202	154

(last checking on 19th october)

Freezer

PCR products

PCR products
Number	Name	Oligo F	Oligo R	Template	Length	Comments	Product Left ?	[DNA]µg/mL	Date
PCR124	pFlgA	O100	O101	-	261pb		yes	0.5	August 7
PCR125	pFlgB	O102	O103	-	261pb		yes	0.5	August 7
PCR126	pFlhB	O108	O109	-	260pb		yes	0.5	August 7
PCR127	FlhDC	O132	O131	MG1655			no		August 8
PCR128	OmpR*	O138	O139	Strain OmpR*	700pb		yes	150	August 8
PCR129	EnvZ*	O126	O127	Strain EnvZ*	1400pb		yes	100	August 8
PCR130	E0240 rbs+	O141	O140	MP143	900pb		no		August 11
PCR131	FlhD rbs-	O132	O133	MG1655	350pb		no		August 11
PCR132	pFlhD rbs-	O130	O131	MG1655	600pb		no		August 11
PCR133	FlhDC + prom	O110	O131	MG1655	1300pb		no		August 11
PCR134	FlhDC + prom	O111	O131	MG1655			no		August 11
PCR135	pFliL	O124	O125	MG1655			yes		August 11
PCR136	pFlhDC	O110	O113	MG1655			yes		August 11
PCR137	pFlhDC	O 111	O 113	MG1655			yes		August 11
PCR138	FlhDC (RBS -)	O 132	O 131	MG 1655			yes		August 15
PCR139	pFlhDC (URI)	O 110	O 113	PCR 130			yes		August 15
PCR140	FlhDC (RBS -)	O 132	O 131	PCR 130			yes		August 15
PCR141	Gene FlhDC + rbs nat	O 134	O 131	MG1655	1000pb		yes		August 26
PCR142	pFliA + rbs nat	O 145	O 144	MG1655	325pb		yes		August 26
PCR143	pFliA sans rbs	O 145	O 146	MG1655	310pb		yes		August 26
PCR144	pFliA + Gene FliA	O 145	O 151	MG1655	1100pb		yes		August 26
PCR145	PCRFliA1	O 148	O 152	MG1655	150pb		yes		August 26
PCR146	PCRFliA2	O 153	O 150	MG1655	670pb		yes		August 26
PCR 147	EnvZ*	O 126	O 127	S 120	1412 bp		yes	150	August 26
PCR 148	OmpR*	O 138	O 139	S 119	789 bp		yes	101	August 26
PCR 149	pFlhDC (Soutourina +RBS)	O 154	O 113	MG1655	450 bp		yes		August 26
PCR 151	pFLiA without nat. rbs	O 145	O 146	MG1655	310 bp		yes	34	August 26
PCR 152	pFliA rbs nat.	O 145	O 144	MG1655	325 bp		yes	19	August 26
PCR 153	pFliA+Gene FliA non-mutated	O 145	O 151	MG1655	1100 bp		yes	39	August 26
PCR 154	Gene FliA mutated (EcoRI/PstI)	O 148	O 150	MG1655	800 bp	154.1, 154.2, 154.3	yes	50	August 26

Biobricks

BioBricks
Name/N°	Plasmid	Strain	Miniprep	Description	comment	Date
[http://partsregistry.org/Part:BBa_B0034 B0034 ]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S107	MP100	Strongest RBS (Efficiency = 1)		July 26
[http://partsregistry.org/Part:BBa_J23101 J23101]	[http://partsregistry.org/Part:BBa_J61002 J61002 ]	S109	MP101	Strongest Constitutive promoter		July 22, July 23, July 26
[http://partsregistry.org/Part:BBa_J23109 J23109]	[http://partsregistry.org/Part:BBa_J61002 J61002]	S110	MP102	Weak Constitutive promoter		July 22, July 26
[http://partsregistry.org/Part:BBa_R0079 R0079]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S108	MP103	promoter pLas (lasr & PAI regulated)		July 23, July 26
[http://partsregistry.org/Part:BBa_R0040 R0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S111	MP104	tetR repressible promoter		July 23, July 26
[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S112	MP105	B0034(rbs) ->LasI		July 26
[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S113	MP106	B0034(rbs) -> TetR		August 24
[http://partsregistry.org/Part:BBa_C0079 C0079]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	S114	MP107	lasR activator with LVA Tag		July 23
[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S115	MP108	lasR activator		July 22, July 23, July 26
[http://partsregistry.org/Part:BBa_E0030 E0030]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S116	MP109	YFP		July 26
[http://partsregistry.org/Part:BBa_E0040 E0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S117	MP110	GFP		July 23, July 26
[http://partsregistry.org/Part:BBa_E1010 E1010 ]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	S118	MP111	mRFP		July 23, July 26
OmpR M57I		S119	MP112	OmpR		July 23
EnvZ I281S		S120	MP113	EnvZ		July 23
[http://partsregistry.org/Part:BBa_C0040 C0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S121	MP114	tetR repressor		July 23
[http://partsregistry.org/Part:pSB1A3 pSB1A3]	[http://partsregistry.org/Part:pSB1A3 pSB1A3]	S122	MP115	plasmid		July 23
[http://partsregistry.org/Part:BBa_J23100 J23100]	[http://partsregistry.org/Part:BBa_J61002 J61002]	x	MP116	Strong constitutive promoter		July 26
[http://partsregistry.org/Part:BBa_J23107 J23107]	[http://partsregistry.org/Part:BBa_J61002 J61002]	x	MP117	Medium constitutive promoter		July 24, July 26
[http://partsregistry.org/Part:BBa_B0015 B0015]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	x	MP118	Double terminator		July 24, July 26
[http://partsregistry.org/Part:BBa_I0500 I0500]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	S103	MP119	align="center"\|AraC pBAD		July 24, July 26
[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	x	MP120	Strong RBS (Efficiency = 0,6)		July 26
[http://partsregistry.org/Part:BBa_E0422 E0422]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S164	MP121	ECFP (RBS+LVA+Double Term)		July 24, July 26
[http://partsregistry.org/Part:BBa_E0840 E0840]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S106	MP122	gfp tri-part; strong rbs		July 26
[http://partsregistry.org/Part:BBa_J61002 J61002]	[http://partsregistry.org/Part:BBa_J61002 J61002]	S142	MP123	plasmid		August 5
[http://partsregistry.org/Part:pSB3K3 pSB3K3]	[http://partsregistry.org/Part:pSB3K3 pSB3K3] (low to medium copy plasmid with p15A ORI / kanamycine resistance)	x	MP142			August 8
[http://partsregistry.org/Part:BBa_E0240 E0240]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	x	MP143	gfp generator		August 8
[http://partsregistry.org/Part:BBa_B0032 B0032]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S163	MP163	medium rbs (efficiency = 0,3)		August 20
[http://partsregistry.org/Part:BBa_E0422 E0422]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	x	MP164	RBS+ ECFP+ LVA+ term		August 20
[http://partsregistry.org/Part:BBa_E0430 E0430]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S165	MP165		RBS+ YFP+ LVA- term		August 20
[http://partsregistry.org/Part:BBa_E0432 E0432]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S166	MP166	RBS+ YFP LVA+ term	BUT be careful , it doesn't work. See registry	August 20
MP167	[http://partsregistry.org/Part:BBa_E0420 E0420]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S167	MP167	RBS+ ECFP LVA- term		August 20
MP168	[http://partsregistry.org/Part:BBa_I732078 I732078]	[http://partsregistry.org/Part:pSB1A3 pSB1A3]	S168	MP168	RBS+ mRFP LVA+ term		August 20
pFlhB	pCR4Blunt-TOPO	x	MP175	pFlhB-RBS (WT)		September 04
pFliA	pCR4Blunt-TOPO	x	MP176	pFliA-RBS (WT) with conserved internal Pst1 restriction site		September 04
pFlhDC	pCR4Blunt-TOPO	x	MP177	pFlhDC-RBS (WT)		September 04
style="background: #D4E2EF;" pFliL	pCR4Blunt-TOPO	x	MP178	pFliL-RBS (WT)		September 04
RBS WT-FlhDC	pCR4Blunt-TOPO	x	MP179	RBS(FlhDC) - FlhDC	with conserved Pst1 site	September 04
FliA2	pCR4Blunt-TOPO	S183	MP180	FliA2	mutated at EcoR1 and Pst1 internal site	September 04
FliA3	pCR4Blunt-TOPO	x	MP181	>br>FliA3	mutated at EcoR1 and Pst1 site	September 04
EnvZ*	pCR4Blunt-TOPO	x	MP182	EnvZ*	the good one but not mutated for EcoR1 site	September 04
OmpR WT	pCR4Blunt-TOPO	x	MP183	OmpR WT	not the mutated	September 04
pFlgB	pCR4Blunt-TOPO	S187	MP184	pFlgB-RBS (WT)		September 04
OmpR WT		x	MP185	OmpR WT	not the mutated	September 04

Ligations: Constructions realised

Constructions
Name/N°	Plasmid	Strain	Miniprep	Description	Digestion	comment	Date
L100	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S146	MP147	rbs TetR - ECFP	D110 (BV) - D130 (BI)		August 13
L101	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S147	MP148	rbs TetR - GFP tripart	D110 (BV) - D131 (BI)	DO NOT WORK !!!!	August 13
L102	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S123	MP124	Strong rbs - YFP	D129 (BV) - D118 (BI)		August 1
L103	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S124	MP125	Strong rbs - mRFP	D129 (BV) - D122 (BI)		August 1
L104	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S125	MP126	Strong rbs - lasR activator	D129 (BV) - D114 (BI)		August 1
L105	[http://partsregistry.org/Part:J61002 J61002]	S126	MP127	Strong promoter - ECFP	D123 (BV) - D130 (BI)		August 1
L106	[http://partsregistry.org/Part:J61002 J61002]	S127	MP128	Strong promoter - gfp Tripart	D123 (BV) - D131 (BI)		August 1
L107	[http://partsregistry.org/Part:J61002 J61002]	S128	MP129	Strong promoter - ECFP	D103 (BV) - D130 (BI)		August 1
L108	[http://partsregistry.org/Part:J61002 J61002]	S129	MP130	Strong promoter - gfp Tripart	D103 (BV) - D131 (BI)		August 1
L109							August
L110	[http://partsregistry.org/Part:J61002 J61002]	S130	MP131	Medium promoter - gfp Tripart	D124 (BV) - D131 (BI)		August 1
L111	[http://partsregistry.org/Part:J61002 J61002]	S131	MP132	Weak promoter - ECFP	D104 (BV) - D130 (BI)		August 1
L112	[http://partsregistry.org/Part:J61002 J61002]	S132	MP133	Weak promoter - GFP tripart	D104 (BV) - D131 (BI)		August 1
L113							August
L114	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	S148	MP149	AracpBAD - gfp tripart	D126 (BV) - D131 (BI)		August 13
L115	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S133	MP134	pLas - ECFP	D105 (BV) - D130 (BI)		August 1
L116	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S134	MP135	pLas - gfp Tripart	D105 (BV) - D131 (BI)		August 1
L117	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S135	MP136	yfp - Double Terminator	D125 (FV) - D117 (FI)		August 1
L118	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S136	MP137	rfp - Double Terminator	D125 (FV) - D121 (FI)		August 1
L119	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S137	MP138	lasR activator + LVA - Double Terminator	D125 (FV) - D113 (FI)		August 1
L120	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S149	MP150	tetR repressible promoter - ECFP	D106 (BV) - D130 (BI)		August 13
L121	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S138	MP139	pTet - gfp tripart	D106 (BV) - D131 (BI)		August 1
L122	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S150	MP151	RBS-lasI - ECFP	D107 (BV) - D130 (BI)		August 13
L123	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S151	MP152	RBS lasI - gfp tripart	D107 (BV) - D131 (BI)		August 13
L124	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S139	MP140	Strongest RBS - rfp	D102 (BV) - D122 (BI)		August 1
L125	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S140	MP141	Strongest RBS - yfp	D102 (BV) - D118 (BI)		August 1
L126	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S152	MP153	Strongest RBS (1)- LasR activator (+LVA)	D102 (BV) - D114 (BI)		August 13
L128	[http://partsregistry.org/Part:J61002 J61002]	S143	MP144	pFlgA-RFP	D132 (FI) - D136 (FV)		August 12
L129	[http://partsregistry.org/Part:J61002 J61002]	S144	MP145	pFlgB-RFP	D133 (FI) - D136 (FV)		August 12
L130	[http://partsregistry.org/Part:J61002 J61002]	S145	MP146	pFlhB-RFP	D134 (FI) - D136 (FV)	wrong sequencing	August 12
L131	[http://partsregistry.org/Part:J61002 J61002]			pFlhDC-RFP	D136 (FV)- D135 (FI)	wrong sequencing	August 8
L132	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S153	MP154	flhDC	D142(FV) - D139(FI)		August 13
L133	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S154	MP155	OmpR*	D142(FV) - D140(FI)	WT sequence (August 19)	August 13 ; August 19
L134	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S155	MP156	EnvZ*	D142(FV) - D141(FI)	lacks about 300 bp in EnvZ* (August 19)	August 13 August 19
L135	[http://partsregistry.org/Part:pSB3K3 pSB3K3]			pFlgA	D137 (BV) - D133 (BI)	don't work	August 9
L136	[http://partsregistry.org/Part:pSB3K3 pSB3K3]			pFlgB	D137 (BV) - D1343 (BI)	don't work	August 9
L137	[http://partsregistry.org/Part:pSB3K3 pSB3K3]			pFlhB	D137 (BV) - D135 (BI)	don't work	August 9
L138	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	S156	MP157	gfp generator (E0240)	D137(FV) - D138(FI)		August 13
L139	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	S159	MP158	E0240 RBS+	D140 (PstI) - D144 (ES)	seems to have the plasmid without the insert and one with the insert	August 15
L140	[http://partsregistry.org/Part:pSB1A2 pSB1A2]			flhD	D145 (V) - D141 (I)		August 12
L141	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S160	MP159	flhC	D142 (ES) - D145 (ES)	has not been sequenced	August 15
L142	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S161	MP160	flhDC+promotor	D143 (ES) - D145 (ES)	does not have the insert	August 15
L143	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	x	x	pFliL in pSB3K3	D149 - D137	didn't work	August 14
L144	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	x	x	pFliL in pSB3K3	D150 - D152	didn't work	August 14
L145	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	x	x	g FlhDC in pSB1A2	D1543 - D145	didn't work	August 16
L146	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	x	x	g FlhDC in pSB1A2	D154 - D145	didn't work	August 16
L147	[http://partsregistry.org/Part:J61002 J61002]	x	x	pFlhDC in J61002	D155 - D136	didn't work	August 16
L148	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	x	x	rbs (B0034)- OmpR*	D158 - D102	didn't work	August 16
L149	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	x	x	rbs (B0034)- EnvZ	D159 - D102	didn't work	August 16
L150	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	x	MP161	pLas + rbs-tetR-gfp tripart	D105 (BV) - D146 (BI)	incorrect clone -> to do again	August 19
L151	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	S162	MP162	rbs-lasR + double terminator	D147 (FI) - D125 (FV)		August 19
L153	[http://partsregistry.org/Part:BBa_J61002 J61002]	S169	MP169	pJ23100-rbs-lasER-dble ter	D123 (BV) - D165 (BI)		August 20
L154	[http://partsregistry.org/Part:BBa_J61002 J61002]	S170	MP170	pJ23101-rbs-lasER-dble ter	D103 (BV) - D165 (BI)		August 20
L155				pJ23101-gfp generator	D164(FV) - D163 (FI)		August 22
L156				pTet-gfp generator	D161 (FV) - D163 (FI)		August 22
L157				tetR-rbs (B0015)	D125 (BV) - D162 (BI)		August 23
L158		x	x	rbs-tetR-gfp generator	D110(BV) - D131 (BI)	didn't work	August 23
L159		x	x	rbs-lasI-dbl ter	D125 (FV) - D109 (FI)	didn't work	August
L160	[http://partsregistry.org/Part:BBa_J61002 J61002]	S174	MP173	pFlgA - YFP Tripart (LVA-)	D166 (FV) - D132 (FI)	Fluorescence OK	August 22
L161	[http://partsregistry.org/Part:BBa_J61002 J61002]	S175	MP174	pFlgA - YFP Tripart (LVA+)	D167 (FV) - D132 (FI)		August 22
L162	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	x	x	rbs-TetR-gfp generator	D110 (BV) - D163 (BI)		August 22
L163	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	x	x	rbs-lasI-gfp generator	D107 (BV) - D163 (BI)		August 22
L166	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S173	MP172	RBS(B0032) - tetR			August 25
L167	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	S172	MP171	gfp generator(E0240) - pTet			August 25
L174	[http://partsregistry.org/Part:J61002 J61002]	S	MP202	Strong constitutive promoter-GFP generator	J23101-E0240		September 26
L176					D125 (v) + D162 (i)		September
L179	[http://partsregistry.org/Part:J61002 J61002]	S200	MP200	J23101 + RBS (B0032) + tet R			September 24
L180					D166 (v) + D109.2 (i)		September
L181					D199 (v) + D109.2 (i)		September
L182					D187 (v) + D109.2 (i)		September
L186					D179 (v) + D205 (i)		September
L190 ("L4")					D199 (v) + D (i)		September
L195 ("L9")					D187 (v) + D194 (i)		September
L ([http://partsregistry.org/Part:BBa_J23100 J23100]-[http://partsregistry.org/Part:BBa_B0034 B0034 ]-[http://partsregistry.org/Part:BBa_C0079 C0079]-[http://partsregistry.org/Part:BBa_B0015 B0015])	[http://partsregistry.org/Part:J61002 J61002]	S169	MP169	strong constitutive promoter-RBS-LasR-term			August 21
L ([http://partsregistry.org/Part:BBa_J23101 J23101]-[http://partsregistry.org/Part:BBa_B0034 B0034 ]-[http://partsregistry.org/Part:BBa_C0079 C0079]-[http://partsregistry.org/Part:BBa_B0015 B0015])	[http://partsregistry.org/Part:J61002 J61002]	S170	MP170	strong constitutive promoter-RBS-LasR-term			August 21
L	pCR4Blunt-TOPO	S178	MP175	pFlhB-RBS (WT)			September 04
L	pCR4Blunt-TOPO	S179	MP176	pFliA-RBS (WT)		pFliA with Pst1 site	September 04
L	pCR4Blunt-TOPO	S180	MP177	pFlhDC-RBS (WT)			September 04
L	pCR4Blunt-TOPO	S181	MP178	pFliL-RBS (WT)			September 04
L	pCR4Blunt-TOPO	S182	MP179	RBS(FlhDC) - FlhDC		FlhDC with conserved Pst1 site	September 04
L	pCR4Blunt-TOPO	S179	MP180	FliA		mutated at EcoR1 and Pst1 site	September 04
L	pCR4Blunt-TOPO	S184	MP181	FliA3		FliA mutated at EcoR1 and Pst1 site	September 04
L	pCR4Blunt-TOPO	S185	MP182	EnvZ*		the good one but not mutated for EcoR1 site	September 04
L	pCR4Blunt-TOPO	S186	MP183	OmpR WT		OmpR WT not the mutated	September 04
L	pCR4Blunt-TOPO	S	MP184	pFlgB-RBS (WT)			September 04
L	pCR4Blunt-TOPO	S188	MP185	OmpR WT		OmpR WT not the mutated	September 04
L	[http://partsregistry.org/Part:J61002 J61002]	S201	MP201	pFlgA(RBS WT)-EnvZ*			September 24
L	[http://partsregistry.org/Part:J61002 J61002]	S207	MP207	pFliA			October 04
L	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	S208	MP208	pFlgA(RBS WT)-EnvZ*		without pFlgA indeed	October 04
L	[http://partsregistry.org/Part:J61002 J61002]	S209	MP209	pFlgA(RBS WT)-EnvZ*-mRFP tripart LVA+		without pFlgA indeed	October 04
L	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	S210	MP210	pFliL(RBS WT)			October 10
L	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	S211	MP211	pFlgA(RBS WT)			October 10
L	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	S212	MP212	pFlhB(RBS WT)			October 10
L	[http://partsregistry.org/Part:BBa_J61002 J61002]	S213	MP213	pFlhB(RBS WT)-mRFP pFlhB(RBS WT)-mRFP			October 10
L	[http://partsregistry.org/Part:BBa_J61002 J61002]	S214	MP214	pFliL(RBS WT)-mRFP			October 10 - style="background: #dddddd;"	RBS WT-FlhDC	pCR4Blunt-TOPO	x	MP179	RBS(FlhDC) - FlhDC	with conserved Pst1 site	September 04

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products 2008
Number	Name	Plamsid	Biobricks	Description	Products in use/comments	Conc. ug/mL	Date
MP200	J23101 + RBS (B0032) + tet R	[http://partsregistry.org/Part:J61002 J61002]		J23101 + RBS (B0032) + tet R			September 24
MP201	pFlgA-EnvZ*	[http://partsregistry.org/Part:J61002 J61002]	pFlgA-EnvZ*	pFlgA(RBS WT)-EnvZ*			September 24
MP207	pFliA	[http://partsregistry.org/Part:BBa_J61002 J61002]	pFliA	pFliA			October 04
MP208	pFlgA-EnvZ*	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	pFlgA-EnvZ*	pFlgA(RBS WT)-EnvZ*	without pFlgA indeed		October 04
MP209	pFlgA-EnvZ*-[http://partsregistry.org/Part:BBa_I732078 I732078]	[http://partsregistry.org/Part:J61002 J61002]	pFlgA-EnvZ*-mRFP tripart	pFlgA(RBS WT)-EnvZ*-mRFP tripart	without pFlgA indeed		October 04
MP210	pFliL	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	pFliL	pFliL(RBS WT)			October 10
MP211	pFlgA	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	pFlgA	pFlgA(RBS WT)			October 10
MP212	pFlhB	[http://partsregistry.org/Part:pSB3K3 pSB3K3]	pFlhB	pFlhB(RBS WT)			October 10
MP213	pFlhB	[http://partsregistry.org/Part:BBa_J61002 J61002]	pFlhB(RBS WT)-mRFP	pFlhB(RBS WT)-mRFP			October 10
MP214	pFliL	[http://partsregistry.org/Part:BBa_J61002 J61002]	pFliL(RBS WT)-mRFP	pFliL(RBS WT)-mRFP			October 10
MP215		[http://partsregistry.org/Part:pSB4T5 pSB4T5]		pSB4T5 (low copy plasmid with pSC101 ORI / tetracycline resistance)			October 14
MP216	[http://partsregistry.org/Part:BBa_E0434 E0434]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		YFP tripart +AAV			October 15

Digestion Products

(Legend: Vector, Insert) Ø

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	Obs	Size Matrix (bp)	Size BB (bp)	Description	Product in use	[]ng/µL	Date
D100.1	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	34			10	July 26
D100.2	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	34			10	July 26
D101	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	XbaI	FV	2076	15			10	July 29
D102.1	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2077	14			10	July 26 August 15
D102.2	[http://partsregistry.org/Part:BBa_B0034 B0034]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2077	14			10	July 26 August 15
D103.1	[http://partsregistry.org/Part:BBa_J23101 J23101]	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	2100	883			27	July 26
D103.2	[http://partsregistry.org/Part:BBa_J23101 J23101]	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	2100	883			35	July 26
D104.1	[http://partsregistry.org/Part:BBa_J23109 J23109]	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	2100	883			20	July 26
D104.2	[http://partsregistry.org/Part:BBa_J23109 J23109]	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	2100	883			20	July 26
D105.1	[http://partsregistry.org/Part:BBa_R0079 R0079]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2222	14			5	July 26
D105.2	[http://partsregistry.org/Part:BBa_R0079 R0079]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2222	14			5	July 26
D106.1	[http://partsregistry.org/Part:BBa_R0040 R0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2119	14			8	July 26
D106.2	[http://partsregistry.org/Part:BBa_R0040 R0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2119	14			8	July 26
D107	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2750	14		August 23	5 12	July 26 July 23
D108.1	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	707			5	July 29
D108.2	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	707			5	July 29
D109	[http://partsregistry.org/Part:BBa_S03154 S03154]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2056	708		July 26	10	July 26
D110	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2768	14		August 23	20 8 19	July 26 August 23 August 28
D111.1	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	725			20	July 26
D111.2	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	725			20	July 26
D112	[http://partsregistry.org/Part:BBa_S03879 S03879]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2079	703			8	September 5
D113	[http://partsregistry.org/Part:BBa_C0079 C0079]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	EcoRI	SpeI	FI	4402	779			20	July 26
D114	[http://partsregistry.org/Part:BBa_C0079 C0079]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	XbaI	PstI	BI	4003	778			20	July 26
D115.1	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	4402	746			-	July 29
D115.2	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	4402	746			-	July 29
D116.1	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	4403	745			20	July 29
D116.1	[http://partsregistry.org/Part:BBa_C0179 C0179]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	4403	745			20	July 29
D117	[http://partsregistry.org/Part:BBa_E0030 E0030]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	EcoRI	SpeI	FI	3166	746			5	July 29
D118	[http://partsregistry.org/Part:BBa_E0030 E0030]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	XbaI	PstI	BI	3167	745			20	July 26
D119	[http://partsregistry.org/Part:BBa_E0040 E0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2056	743			10	July 26
D120	[http://partsregistry.org/Part:BBa_E0040 E0040]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	742			10	July 26
D121	[http://partsregistry.org/Part:BBa_E1010 E1010]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	EcoRI	SpeI	FI	4402	704			10	July 26
D122	[http://partsregistry.org/Part:BBa_E1010 E1010]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	4403	703			10	July 26
D123.1	[http://partsregistry.org/Part:BBa_J23100 J23100]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			26	July 26
D123.2	[http://partsregistry.org/Part:BBa_J23100 J23100]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			23	July 26
D124.1	[http://partsregistry.org/Part:BBa_J23107 J23107]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			15	July 26
D124.2	[http://partsregistry.org/Part:BBa_J23107 J23107]	[http://partsregistry.org/Part:J61002 J61002]	SpeI	PstI	BV	2100	883			15	July 26
D125.1	[http://partsregistry.org/Part:BBa_B0015 B0015]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	EcoRI	XbaI	FV	3303	15			26 10	July 26 August 23 August 28
D125.2	[http://partsregistry.org/Part:BBa_B0015 B0015]	[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]	EcoRI	XbaI	FV	3303	15			24 3687	July 26 August 23 August 28
D126.1	[http://partsregistry.org/Part:BBa_I0500 I0500]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	SpeI	PstI	BV	5621	14			10	July 26
D126.2	[http://partsregistry.org/Part:BBa_I0500 I0500]	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	SpeI	PstI	BV	5621	14			10	July 26
D127.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	37			10	July 26
D127.2	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	37			10	July 26
D128.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	XbaI	FV	2079	15			10	July 29
D128.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	EcoRI	XbaI	FV	2079	15			10	July 29
D129.1	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2080	14			10	July 29
D129.2	[http://partsregistry.org/Part:BBa_B0030 B0030]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	SpeI	PstI	BV	2080	14			10	July 29
D130.1	[http://partsregistry.org/Part:BBa_E0422 E0422]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	939			10	July 26
D130.2
D130.3
D131.1	[http://partsregistry.org/Part:BBa_E0840 E0840]	[http://partsregistry.org/Part:pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	900			8	July 26
D131.2
D131.3
D132	pFlgA	-	EcoRI	SpeI	FI	-	250			5	August 6
D133	pFlgB	-	EcoRI	SpeI	FI	-	250			5	August 6
D134	pFlhB	-	EcoRI	SpeI	FI	-	249			5	August 6
D135	pFlhDC	-	EcoRI	SpeI	FI	-		problem			August 6
D136	-	[http://partsregistry.org/Part:BBa_J61002 J61002]	EcoRI	SpeI	FV	2925	-				August 6 August 16
D137	pSB3K3	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	EcoRI	SpeI	FV	2727	-				August 6
D138	E0240	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	899	-				August 6
D139	flhDC	-	EcoRI	PstI	FI	-	972				August 6
D140	E040 rbs+	-	PstI	PstI	I	-					August 12
D141	flhD RBS -	-	EcoRI	SpeI	FI	-					August 12
D142	flhC RBS -	-	EcoRI	SpeI	FI	-					August 12
D143	flhDC + promoter	-	EcoRI	SpeI	FI	-					August 12
D144	pSB3K3	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	EcoRI	SpeI	FI	-					August 12
D145	pSB1A2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	-					August 12 August 15
D146	MP148	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI	2057	1609	rbs TetR - GFP tripart D110 (BV) - D131 (BI)			August 15
D147	MP153	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	2056	799	Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)			August 15
D149	pfliL	-	EcoRI	SpeI	FI	-					August 14 August 16
D150	pfliL	-	XbaI	PstI	BI	-					August 14 August 16
D152	pSB3K3	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	XbaI	PstI	BI	-					August 14
D153	PCR 138 - gène flhDC	-	EcoRI	SpeI	FI	-					August 15
D154	PCR 140 - gène flhDC	-	EcoRI	SpeI	FI	-					August 15
D155	PCR 139 - pflhDC (URI)	-	EcoRI	SpeI	FI	-					August 15
D158	MP 155.1 - OmpR*	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI	-					August 15
D159	MP 156.1 - EnvZ*	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI	-					August 15
D161	MP 104 - pTet	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	SpeI	PstI	BV	-				39	August 16
D162	MP 114 - TetR	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI	-				11	August 16
D163	MP 143 - gfp generator	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI	-			August 23	14 11	August 16 August 23
D164	MP 101 - pJ23101	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV	-				3	August 16
D165	MP162.2	[http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3]	XbaI	PstI	BI	2057	798	rbs-lasR - double terminator D147 (FI) - D125 (FV)		27	August 18
D166	MP 165.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV	-	2942	RBS (B0034)+ YFP+ LVA- term B0015		59	August 26 September 3
D179	MP3.4 or MP118	[http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3]	SpeI	PstI	BV			B0015 (double terminator B0010-B0012)			August 20
D180	MP101.1	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI	BV			promoter J23101			August 20
D181	MP104.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV			pTet (TetR repressible promoter)			August 20
D182	MP114.1	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI			TetR			August 20
D183	MP119.3	-	XbaI	PstI	BI			pBad promoter			August 20
D184	MP143.1	[http://partsregistry.org/Part:BBa_pSB2K3 pSB2K3]	EcoRI	SpeI	FI			gfp generator			August 18
D185	MP163.1	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	SpeI	PstI	BV			B0032 RBS			August 18
D187	MP168.1	[http://partsregistry.org/Part:BBa_pSB1A3 pSB1A3]	EcoRI	XbaI	FV			RBS (B0034)+ mRFP LVA+ term B0015		43	September 3
D188	PCR 138	-	XbaI	PstI	Insert	-	767	OmpR*			August 26
D189	MP 101.2	[http://partsregistry.org/Part:BBa_J61002 J61002]	XbaI	PstI	Vector	-	2073	pSB1A2			August 26
D191	MP114.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	SpeI	PstI	BV		2724	tetR		22	August 29
D192	MP144.2	[http://partsregistry.org/Part:BBa_J61002 J61002]	EcoRI	XbaI	FV		2267	FlgA		26	August 29
D193	MP171	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI		961	gfp generator(E0240) - pTet		9,5	August 29
D194	MP172.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	SpeI	FI		704	RBS(B0032) - tetR		13	August 29
D195	MP172.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI		703	RBS(B0032) - tetR		11	August 29
D196	MP104.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	SpeI	PstI	BV		2119	pTet		45	September 3
D197	MP157.2	[http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3]	XbaI	PstI	BI		898	gfp generator(E0240)		12	September 3
D198	MP164.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV		2981	RBS (B0034)+ ECFP+ LVA+ term B0015		36	September 3
D199	MP167.1	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV		2942	RBS (B0034)+ ECFP LVA- term (B0015)		61	September 3
D200	MP171.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	XbaI	PstI	BI		960	gfp generator(E0240) - pTet		43	September 3
D201	MP114.2	[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoRI	XbaI	FV		2724	tetR		13	August 29
D202	MP101.1	[http://partsregistry.org/Part:BBa_J61002 J61002]	EcoRI	nothing			2984			25	September 8
D203	MP101.2	[http://partsregistry.org/Part:BBa_J61002 J61002]	BspHI	nothing			1870 1008 105				September 11
D204	MP144	[http://partsregistry.org/Part:BBa_J61002 J61002]	SpeI	PstI							September 22
D205	MP101	[http://partsregistry.org/Part:BBa_J61002 J61002]	XbaI	PstI							September 22
D206	MP179		XbaI	PstI							September 22
D207	MP182.3		XbaI	PstI							September
D208	MP175		EcoRI	SpeI							September
D209	MP144	[http://partsregistry.org/Part:BBa_J61002 J61002]	EcoRI	SpeI							September
D210	MP178		EcoRI	SpeI							September

Strains (glycerol stock)

Strains glycerol stock
Number	Name	Biobricks	Description	Verified clone	Date
S141	MG1655				Aug 7
S157	[http://partsregistry.org/Part:BBa_E0240 E0240] in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		GFP generator		August 15
S158	[http://partsregistry.org/Part:pSB3K3 pSB3K3]		plasmid		August 15
S171	L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2]		pTet - gfp Tripart D106 (BV) - D131 (BI)	Same as S138 but in chemically labmade competent MG1655 bacteria. For study of doubling time of population	Aug 22
S215	[http://partsregistry.org/Part:pSB4T5 pSB4T5]		plasmid		October 14
S216	[http://partsregistry.org/Part:BBa_E0434 E0434] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]		YFP tripart +AAV		October 15

@@ Line 1,604: / Line 1,604: @@
 |
 |align="center"|[[Team:Paris/October 10|October 10]]
+- style="background: #dddddd;"
+| style="background: #D4E2EF;" |RBS WT-FlhDC
+|align="center"|pCR4Blunt-TOPO
+|align="center"|x
+|align="center"|MP179
+|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]<br>RBS(FlhDC) - FlhDC
+| with conserved Pst1 site
+|align="center"|[[Team:Paris/September 04|September 04]]
 |}

Team:Paris/Notebook/Freezer

From 2008.igem.org

Revision as of 18:03, 20 October 2008

Contents

Last number used

Freezer

PCR products

Biobricks

Ligations: Constructions realised

Plasmids: MiniPrep Products

Digestion Products

Strains (glycerol stock)