Team:Paris/Notebook/Freezer

From 2008.igem.org

(Difference between revisions)
(→Biobricks)
(→Biobricks)
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|align="center"|[[Team:Paris/October 10|October 10]]
|align="center"|[[Team:Paris/October 10|October 10]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;"  |L132<br>D142(FV) - D139(FI)
+
| style="background: #D4E2EF;"  |L132
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|S153
|align="center"|S153
|align="center"|MP154
|align="center"|MP154
|align="center"|[[Image:Icon_coding.png]]<br>flhDC
|align="center"|[[Image:Icon_coding.png]]<br>flhDC
-
|
+
|align="center"|D142(FV) - D139(FI)
|align="center"|[[Team:Paris/August 13|August 13]]
|align="center"|[[Team:Paris/August 13|August 13]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L133<br>D142(FV) - D140(FI)
+
| style="background: #D4E2EF;" |L133
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|S154
|align="center"|S154
|align="center"|MP155
|align="center"|MP155
|align="center"|[[Image:Icon_coding.png]]<BR>OmpR*
|align="center"|[[Image:Icon_coding.png]]<BR>OmpR*
-
|align="center"|WT sequence  
+
|align="center"|D142(FV) - D140(FI)<br>WT sequence  
|align="center"| [[Team:Paris/August 19|August 19]]
|align="center"| [[Team:Paris/August 19|August 19]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L134<br>D142(FV) - D141(FI)
+
| style="background: #D4E2EF;" |L134
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|S155
|align="center"|S155
|align="center"|MP156
|align="center"|MP156
|align="center"|[[Image:Icon_coding.png]]<BR> EnvZ*
|align="center"|[[Image:Icon_coding.png]]<BR> EnvZ*
-
|align="center"|lacks about 300 bp in EnvZ*
+
|align="center"|D142(FV) - D141(FI)<br>lacks about 300 bp in EnvZ*
|align="center"|[[Team:Paris/August 19| August 19]]
|align="center"|[[Team:Paris/August 19| August 19]]
|- style="background: #dddddd;"  
|- style="background: #dddddd;"  
-
|style="background: #D4E2EF;" |L135<br>D137 (BV) - D133 (BI)
+
|style="background: #D4E2EF;" |L135
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|
|align="center"|
|align="center"|
|align="center"|
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlgA
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlgA
-
|align="center"|don't work
+
|align="center"|D137 (BV) - D133 (BI)<br>don't work
|align="center"|[[Team:Paris/August 9|August 9]]
|align="center"|[[Team:Paris/August 9|August 9]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L136<br>D137 (BV) - D1343 (BI)
+
| style="background: #D4E2EF;" |L136
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|
|align="center"|
|align="center"|
|align="center"|
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlgB
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlgB
-
|align="center"|don't work
+
|align="center"|D137 (BV) - D1343 (BI)<br>don't work
|align="center"|[[Team:Paris/August 9|August 9]]
|align="center"|[[Team:Paris/August 9|August 9]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L137<br>D137 (BV) - D135 (BI)
+
| style="background: #D4E2EF;" |L137
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|
|align="center"|
|align="center"|
|align="center"|
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlhB
|align="center"|[[Image:Part_icon_regulatory.png]]<br>pFlhB
-
|align="center"|don't work
+
|align="center"|D137 (BV) - D135 (BI)<br>don't work
|align="center"|[[Team:Paris/August 9|August 9]]
|align="center"|[[Team:Paris/August 9|August 9]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L140<br> D145 (V) - D141 (I)
+
| style="background: #D4E2EF;" |L140
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|
|align="center"|
|align="center"|
|align="center"|
|align="center"|[[Image:Icon_coding.png]]<br>flhD
|align="center"|[[Image:Icon_coding.png]]<br>flhD
-
|
+
|align="center"|D145 (V) - D141 (I)
|align="center"|[[Team:Paris/August 12|August 12]]
|align="center"|[[Team:Paris/August 12|August 12]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L141<br>D142 (ES) - D145 (ES)
+
| style="background: #D4E2EF;" |L141
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|S160
|align="center"|S160
|align="center"|MP159
|align="center"|MP159
|align="center"|[[Image:Icon_coding.png]]<br>flhC
|align="center"|[[Image:Icon_coding.png]]<br>flhC
-
|align="center"|has not been <br>sequenced
+
|align="center"|D142 (ES) - D145 (ES)<br>has not been sequenced
|align="center"|[[Team:Paris/August 15|August 15]]
|align="center"|[[Team:Paris/August 15|August 15]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 811: Line 811:
|align="center"|[[Team:Paris/August 14|August 14 ]]
|align="center"|[[Team:Paris/August 14|August 14 ]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L144<br>D150 - D152
+
| style="background: #D4E2EF;" |L144
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"| x
|align="center"| x
|align="center"| x
|align="center"| x
|align="center"|[[Image:Icon_regulatory.png]]<br>pFliL
|align="center"|[[Image:Icon_regulatory.png]]<br>pFliL
-
|align="center"|didn't work
+
|align="center"|D150 - D152<br>didn't work
|align="center"|[[Team:Paris/August 14|August 14 ]]
|align="center"|[[Team:Paris/August 14|August 14 ]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L145<br> D1543 - D145
+
| style="background: #D4E2EF;" |L145
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"| x
|align="center"| x
|align="center"| x
|align="center"| x
|align="center"|[[Image:Icon_coding.png]]<br>FlhDC
|align="center"|[[Image:Icon_coding.png]]<br>FlhDC
-
|align="center"|didn't work
+
|align="center"|D1543 - D145<br>didn't work
|align="center"|[[Team:Paris/August 16|August 16 ]]
|align="center"|[[Team:Paris/August 16|August 16 ]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L146<br>D154 - D145
+
| style="background: #D4E2EF;" |L146
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"| x
|align="center"| x
|align="center"| x
|align="center"| x
|align="center"|[[Image:Icon_coding.png]]<br>FlhDC
|align="center"|[[Image:Icon_coding.png]]<br>FlhDC
-
|align="center"|didn't work
+
|align="center"|D154 - D145<br>didn't work
|align="center"|[[Team:Paris/August 16|August 16 ]]
|align="center"|[[Team:Paris/August 16|August 16 ]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
-
| style="background: #D4E2EF;" |L147<br> D155 - D136
+
| style="background: #D4E2EF;" |L147
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"| x
|align="center"| x
|align="center"| x
|align="center"| x
|align="center"|[[Image:Icon_regulatory.png]]<br>pFlhDC  
|align="center"|[[Image:Icon_regulatory.png]]<br>pFlhDC  
-
|align="center"|didn't work
+
|align="center"|D155 - D136<br>didn't work
|align="center"|[[Team:Paris/August 16|August 16 ]]
|align="center"|[[Team:Paris/August 16|August 16 ]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   

Revision as of 18:49, 20 October 2008

Contents

Last number used

Miniprep Strain Ligation Digestion PCR
216 216 213 202 154

(last checking on 19th october)

Freezer

PCR products

PCR products
Number Name Oligo F Oligo R Template Length Comments Product Left ? [DNA]µg/mL Date
PCR124 pFlgA O100 O101 - 261pb yes 0.5 August 7
PCR125 pFlgB O102 O103 - 261pb yes 0.5 August 7
PCR126 pFlhB O108 O109 - 260pb yes 0.5 August 7
PCR127 FlhDC O132 O131 MG1655 no August 8
PCR128 OmpR* O138 O139 Strain OmpR* 700pb yes 150 August 8
PCR129 EnvZ* O126 O127 Strain EnvZ* 1400pb yes 100 August 8
PCR130 E0240 rbs+ O141 O140 MP143 900pb no August 11
PCR131 FlhD rbs- O132 O133 MG1655 350pb no August 11
PCR132 pFlhD rbs- O130 O131 MG1655 600pb no August 11
PCR133 FlhDC + prom O110 O131 MG1655 1300pb no August 11
PCR134 FlhDC + prom O111 O131 MG1655 no August 11
PCR135 pFliL O124 O125 MG1655 yes August 11
PCR136 pFlhDC O110 O113 MG1655 yes August 11
PCR137 pFlhDC O 111 O 113 MG1655 yes August 11
PCR138 FlhDC (RBS -) O 132 O 131 MG 1655 yes August 15
PCR139 pFlhDC (URI) O 110 O 113 PCR 130 yes August 15
PCR140 FlhDC (RBS -) O 132 O 131 PCR 130 yes August 15
PCR141 Gene FlhDC + rbs nat O 134 O 131 MG1655 1000pb yes August 26
PCR142 pFliA + rbs nat O 145 O 144 MG1655 325pb yes August 26
PCR143 pFliA sans rbs O 145 O 146 MG1655 310pb yes August 26
PCR144 pFliA + Gene FliA O 145 O 151 MG1655 1100pb yes August 26
PCR145 PCRFliA1 O 148 O 152 MG1655 150pb yes August 26
PCR146 PCRFliA2 O 153 O 150 MG1655 670pb yes August 26
PCR 147 EnvZ* O 126 O 127 S 120 1412 bp yes 150 August 26
PCR 148 OmpR* O 138 O 139 S 119 789 bp yes 101 August 26
PCR 149 pFlhDC (Soutourina +RBS) O 154 O 113 MG1655 450 bp yes August 26
PCR 151 pFLiA without nat. rbs O 145 O 146 MG1655 310 bp yes 34 August 26
PCR 152 pFliA rbs nat. O 145 O 144 MG1655 325 bp yes 19 August 26
PCR 153 pFliA+Gene FliA non-mutated O 145 O 151 MG1655 1100 bp yes 39 August 26
PCR 154 Gene FliA mutated (EcoRI/PstI) O 148 O 150 MG1655 800 bp 154.1, 154.2, 154.3 yes 50 August 26

Biobricks

BioBricks
Name/N° Plasmid Strain Miniprep Description comment Date
[http://partsregistry.org/Part:BBa_B0034 B0034 ] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S107 MP100 Part icon rbs.png
Strongest RBS (Efficiency = 1) 		July 26
[http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 J61002 ] S109 MP101 Part icon regulatory.png
Strongest Constitutive promoter 		July 22, July 23, July 26
[http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] S110 MP102 Part icon regulatory.png
Weak Constitutive promoter 		July 22, July 26
[http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S108 MP103 Part icon regulatory.png
promoter pLas (lasr & PAI regulated) 		July 23, July 26
[http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S111 MP104 Part icon regulatory.png
tetR repressible promoter 		July 23, July 26
[http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S112 MP105 Part icon rbs.pngIcon coding.png
B0034(rbs) ->LasI 		July 26
[http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S113 MP106 Part icon rbs.pngIcon coding.png
B0034(rbs) -> TetR 		August 24
[http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S114 MP107 Icon coding.png
lasR activator with LVA Tag 		July 23
[http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S115 MP108 Icon coding.png
lasR activator 		July 22, July 23, July 26
[http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S116 MP109 Part icon reporter.png
YFP 		July 26
[http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S117 MP110 Part icon reporter.png
GFP 		July 23, July 26
[http://partsregistry.org/Part:BBa_E1010 E1010 ] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S118 MP111 Part icon reporter.png
mRFP 		July 23, July 26
OmpR M57I S119 MP112 Icon coding.png
OmpR 		July 23
EnvZ I281S S120 MP113 Icon coding.png
EnvZ 		July 23
[http://partsregistry.org/Part:BBa_C0040 C0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S121 MP114 Icon coding.png
tetR repressor 		July 23
[http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:pSB1A3 pSB1A3] S122 MP115 Icon plasmid.png
plasmid 		July 23
[http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:BBa_J61002 J61002] x MP116 Part icon regulatory.png
Strong constitutive promoter 		July 26
[http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:BBa_J61002 J61002] x MP117 Part icon regulatory.png
Medium constitutive promoter 		July 24, July 26
[http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] x MP118 Part icon terminator.pngPart icon terminator.png
Double terminator 		July 24, July 26
[http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S103 MP119 Part icon regulatory.png
AraC pBAD 		July 24, July 26
[http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] x MP120 Part icon rbs.png
Strong RBS (Efficiency = 0,6) 		July 26
[http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S164 MP121 Part icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
ECFP (RBS+LVA+Double Term) 		July 24, July 26
[http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S106 MP122 Part icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
gfp tri-part; strong rbs 		July 26
[http://partsregistry.org/Part:BBa_J61002 J61002] [http://partsregistry.org/Part:BBa_J61002 J61002] S142 MP123 Icon plasmid.png
plasmid 		August 5
[http://partsregistry.org/Part:pSB3K3 pSB3K3] [http://partsregistry.org/Part:pSB3K3 pSB3K3] x MP142 Icon plasmid.png
plasmid 		low to medium copy plasmid with p15A ORI August 8
[http://partsregistry.org/Part:BBa_E0240 E0240] [http://partsregistry.org/Part:pSB1A2 pSB1A2] x MP143 Part icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
gfp generator 		August 8
[http://partsregistry.org/Part:BBa_B0032 B0032] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S163 MP163 Icon rbs.png
medium rbs (efficiency = 0,3) 		August 20
[http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] x MP164 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ ECFP+ LVA+ term 		August 20
[http://partsregistry.org/Part:BBa_E0430 E0430] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S165 MP165 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ YFP+ LVA- term 		August 20
[http://partsregistry.org/Part:BBa_E0432 E0432] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S166 MP166 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ YFP LVA+ term 		BUT be careful , it doesn't work. See registry August 20
[http://partsregistry.org/Part:BBa_E0420 E0420] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S167 MP167 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ ECFP LVA- term 		August 20
[http://partsregistry.org/Part:BBa_I732078 I732078] [http://partsregistry.org/Part:pSB1A3 pSB1A3] S168 MP168 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS+ mRFP LVA+ term 		August 20
pFlhB pCR4Blunt-TOPO x MP175 Icon regulatory.pngIcon rbs.png
pFlhB-RBS (WT) 		September 04
pFliA pCR4Blunt-TOPO x MP176 Icon regulatory.pngIcon rbs.png
pFliA-RBS (WT) 		with conserved Pst1 site September 04
pFlhDC pCR4Blunt-TOPO x MP177 Icon regulatory.pngIcon rbs.png
pFlhDC-RBS (WT) 		September 04
pFliL pCR4Blunt-TOPO x MP178 Icon regulatory.pngIcon rbs.png
pFliL-RBS (WT) 		September 04
FliA2 pCR4Blunt-TOPO S183 MP180 Icon coding.png
FliA2 		mutated at EcoR1 and Pst1 internal site September 04
FliA3 pCR4Blunt-TOPO x MP181 Icon coding.png
FliA3 		mutated at EcoR1 and Pst1 site September 04
EnvZ* pCR4Blunt-TOPO x MP182 Icon coding.png
EnvZ* 		the good one but not mutated for EcoR1 site September 04
OmpR WT pCR4Blunt-TOPO x MP183 Icon coding.png
OmpR WT 		not the mutated September 04
pFlgB pCR4Blunt-TOPO S187 MP184 Icon regulatory.pngIcon rbs.png
pFlgB-RBS (WT) 		September 04
OmpR WT x MP185 Icon coding.png
OmpR WT 		not the mutated September 04
L [http://partsregistry.org/Part:pSB3K3 pSB3K3] S210 MP210 Icon regulatory.pngIcon rbs.png
pFliL(RBS WT) 		October 10
L [http://partsregistry.org/Part:pSB3K3 pSB3K3] S211 MP211 Icon regulatory.pngIcon rbs.png
pFlgA(RBS WT) 		October 10
L [http://partsregistry.org/Part:pSB3K3 pSB3K3] S212 MP212 Icon regulatory.pngIcon rbs.png
pFlhB(RBS WT) 		October 10
L132 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S153 MP154 Icon coding.png
flhDC 		D142(FV) - D139(FI) August 13
L133 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S154 MP155 Icon coding.png
OmpR* 		D142(FV) - D140(FI)
WT sequence 		August 19
L134 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S155 MP156 Icon coding.png
EnvZ* 		D142(FV) - D141(FI)
lacks about 300 bp in EnvZ* 		August 19
L135 [http://partsregistry.org/Part:pSB3K3 pSB3K3] Part icon regulatory.png
pFlgA 		D137 (BV) - D133 (BI)
don't work 		August 9
L136 [http://partsregistry.org/Part:pSB3K3 pSB3K3] Part icon regulatory.png
pFlgB 		D137 (BV) - D1343 (BI)
don't work 		August 9
L137 [http://partsregistry.org/Part:pSB3K3 pSB3K3] Part icon regulatory.png
pFlhB 		D137 (BV) - D135 (BI)
don't work 		August 9
L140 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png
flhD 		D145 (V) - D141 (I) August 12
L141 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S160 MP159 Icon coding.png
flhC 		D142 (ES) - D145 (ES)
has not been sequenced 		August 15
L143
D149 - D137 		[http://partsregistry.org/Part:pSB3K3 pSB3K3] x x Icon regulatory.png
pFliL 		didn't work August 14
L144 [http://partsregistry.org/Part:pSB3K3 pSB3K3] x x Icon regulatory.png
pFliL 		D150 - D152
didn't work 		August 14
L145 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x x Icon coding.png
FlhDC 		D1543 - D145
didn't work 		August 16
L146 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x x Icon coding.png
FlhDC 		D154 - D145
didn't work 		August 16
L147 [http://partsregistry.org/Part:J61002 J61002] x x Icon regulatory.png
pFlhDC 		D155 - D136
didn't work 		August 16
L pCR4Blunt-TOPO S179 MP176 Icon regulatory.pngIcon rbs.png
pFliA-RBS (WT) 		pFliA with Pst1 site September 04
L pCR4Blunt-TOPO S180 MP177 Icon regulatory.pngIcon rbs.png
pFlhDC-RBS (WT) 		September 04
L pCR4Blunt-TOPO S181 MP178 Icon regulatory.pngIcon rbs.png
pFliL-RBS (WT) 		September 04
L pCR4Blunt-TOPO S182 MP179 Part icon rbs.pngIcon coding.png
RBS(FlhDC) - FlhDC 		FlhDC with conserved Pst1 site September 04
L pCR4Blunt-TOPO S179 MP180 Icon coding.png
FliA 		mutated at EcoR1 and Pst1 site September 04
L pCR4Blunt-TOPO S184 MP181 Icon coding.png
FliA3 		FliA mutated at EcoR1 and Pst1 site September 04
L pCR4Blunt-TOPO S185 MP182 Icon coding.png
EnvZ* 		the good one but not mutated for EcoR1 site September 04
L pCR4Blunt-TOPO S186 MP183 Icon coding.png
OmpR WT 		OmpR WT not the mutated September 04
L [http://partsregistry.org/Part:J61002 J61002] S207 MP207 Icon regulatory.png
pFliA 		October 04
L pCR4Blunt-TOPO S188 MP185 Icon coding.png
OmpR WT 		OmpR WT not the mutated September 04

Devices

Ligations: Constructions realised
Name/N° Plasmid Strain Miniprep Description Digestion comment Date
L100 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S146 MP147 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
rbs TetR - ECFP 		D110 (BV) - D130 (BI) August 13
L101 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S147 MP148 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
rbs TetR - GFP tripart 		D110 (BV) - D131 (BI) DO NOT WORK !!!! August 13
L102 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S123 MP124 Part icon rbs.pngPart icon reporter.png
Strong rbs - YFP 		D129 (BV) - D118 (BI) August 1
L103 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S124 MP125 Part icon rbs.pngPart icon reporter.png
Strong rbs - mRFP 		D129 (BV) - D122 (BI) August 1
L104 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S125 MP126 Part icon rbs.pngIcon coding.png
Strong rbs - lasR activator 		D129 (BV) - D114 (BI) August 1
L105 [http://partsregistry.org/Part:J61002 J61002] S126 MP127 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strong promoter - ECFP 		D123 (BV) - D130 (BI) August 1
L106 [http://partsregistry.org/Part:J61002 J61002] S127 MP128 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strong promoter - gfp Tripart 		D123 (BV) - D131 (BI) August 1
L107 [http://partsregistry.org/Part:J61002 J61002] S128 MP129 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strong promoter - ECFP 		D103 (BV) - D130 (BI) August 1
L108 [http://partsregistry.org/Part:J61002 J61002] S129 MP130 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Strong promoter - gfp Tripart 		D103 (BV) - D131 (BI) August 1
L109 August
L110 [http://partsregistry.org/Part:J61002 J61002] S130 MP131 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Medium promoter - gfp Tripart 		D124 (BV) - D131 (BI) August 1
L111 [http://partsregistry.org/Part:J61002 J61002] S131 MP132 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Weak promoter - ECFP 		D104 (BV) - D130 (BI) August 1
L112 [http://partsregistry.org/Part:J61002 J61002] S132 MP133 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
Weak promoter - GFP tripart 		D104 (BV) - D131 (BI) August 1
L113 August
L114 [http://partsregistry.org/Part:pSB2K3 pSB2K3] S148 MP149 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
AracpBAD - gfp tripart 		D126 (BV) - D131 (BI) August 13
L115 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S133 MP134 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - ECFP 		D105 (BV) - D130 (BI) August 1
L116 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S134 MP135 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas - gfp Tripart 		D105 (BV) - D131 (BI) August 1
L117 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S135 MP136 Part icon reporter.pngPart icon terminator.pngPart icon terminator.png
yfp - Double Terminator 		D125 (FV) - D117 (FI) August 1
L118 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S136 MP137 Part icon reporter.pngPart icon terminator.pngPart icon terminator.png
rfp - Double Terminator 		D125 (FV) - D121 (FI) August 1
L119 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S137 MP138 Icon coding.pngPart icon terminator.pngPart icon terminator.png
lasR activator + LVA - Double Terminator 		D125 (FV) - D113 (FI) August 1
L120 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S149 MP150 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
tetR repressible promoter - ECFP 		D106 (BV) - D130 (BI) August 13
L121 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S138 MP139 Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
pTet - gfp tripart 		D106 (BV) - D131 (BI) August 1
L122 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S150 MP151 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS-lasI - ECFP 		D107 (BV) - D130 (BI) August 13
L123 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S151 MP152 Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png
RBS lasI - gfp tripart 		D107 (BV) - D131 (BI) August 13
L124 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S139 MP140 Part icon rbs.pngPart icon reporter.png
Strongest RBS - rfp 		D102 (BV) - D122 (BI) August 1
L125 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S140 MP141 Part icon rbs.pngPart icon reporter.png
Strongest RBS - yfp 		D102 (BV) - D118 (BI) August 1
L126 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S152 MP153 Part icon rbs.pngIcon coding.png
Strongest RBS (1)- LasR activator (+LVA) 		D102 (BV) - D114 (BI) August 13
L128 [http://partsregistry.org/Part:J61002 J61002] S143 MP144 Part icon regulatory.pngPart icon reporter.png
pFlgA-RFP 		D132 (FI) - D136 (FV) August 12
L129 [http://partsregistry.org/Part:J61002 J61002] S144 MP145 Part icon regulatory.pngPart icon reporter.png
pFlgB-RFP 		D133 (FI) - D136 (FV) August 12
L130 [http://partsregistry.org/Part:J61002 J61002] S145 MP146 Part icon regulatory.pngPart icon reporter.png
pFlhB-RFP 		D134 (FI) - D136 (FV) wrong sequencing August 12
L131 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png
pFlhDC-RFP 		D136 (FV)- D135 (FI) wrong sequencing August 8
L138 [http://partsregistry.org/Part:pSB3K3 pSB3K3] S156 MP157 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
gfp generator (E0240) 		D137(FV) - D138(FI) August 13
L139 [http://partsregistry.org/Part:pSB3K3 pSB3K3] S159 MP158 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
E0240 RBS+ 		D140 (PstI) - D144 (ES) seems to have the
plasmid without the
insert and one
with the insert 		August 15
L142 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S161 MP160 Icon regulatory.pngIcon rbs.pngIcon coding.png
flhDC+promotor 		D143 (ES) - D145 (ES) does not have
the insert 		August 15
L148 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x x Icon rbs.pngIcon coding.png
rbs (B0034)- OmpR* 		D158 - D102 didn't work August 16
L149 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x x Icon rbs.pngIcon coding.png
rbs (B0034)- EnvZ 		D159 - D102 didn't work August 16
L150 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x MP161 Icon regulatory.pngIcon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pLas + rbs-tetR-gfp tripart 		D105 (BV) - D146 (BI) incorrect clone
-> to do again 		August 19
L151 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S162 MP162 Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
rbs-lasR + double terminator 		D147 (FI) - D125 (FV) August 19
L153 [http://partsregistry.org/Part:BBa_J61002 J61002] S169 MP169 Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
pJ23100-rbs-lasER-dble ter 		D123 (BV) - D165 (BI) August 20
L154 [http://partsregistry.org/Part:BBa_J61002 J61002] S170 MP170 Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
pJ23101-rbs-lasER-dble ter 		D103 (BV) - D165 (BI) August 20
L155 Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pJ23101-gfp generator 		D164(FV) - D163 (FI) August 22
L156 Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pTet-gfp generator 		D161 (FV) - D163 (FI) August 22
L157 Icon coding.pngIcon rbs.png
tetR-rbs (B0015) 		D125 (BV) - D162 (BI) August 23
L158 x x Icon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
rbs-tetR-gfp generator 		D110(BV) - D131 (BI) didn't work August 23
L159 x x Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
rbs-lasI-dbl ter 		D125 (FV) - D109 (FI) didn't work August
L160 [http://partsregistry.org/Part:BBa_J61002 J61002] S174 MP173 Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA - YFP Tripart (LVA-) 		D166 (FV) - D132 (FI) Fluorescence OK August 22
L161 [http://partsregistry.org/Part:BBa_J61002 J61002] S175 MP174 Icon regulatory.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA - YFP Tripart (LVA+) 		D167 (FV) - D132 (FI) August 22
L162 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] x x Icon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
rbs-TetR-gfp generator 		D110 (BV) - D163 (BI) August 22
L163 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] x x Icon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
rbs-lasI-gfp generator 		D107 (BV) - D163 (BI) August 22
L166 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S173 MP172 Part icon rbs.pngIcon coding.png
RBS(B0032) - tetR 		August 25
L167 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S172 MP171 Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png
gfp generator(E0240) - pTet 		August 25
L174 [http://partsregistry.org/Part:J61002 J61002] S MP202
Icon regulatory.pngIcon coding.png
Strong constitutive promoter-GFP generator 		J23101-E0240 September 26
L176 D125 (v) + D162 (i) September
L179 [http://partsregistry.org/Part:J61002 J61002] S200 MP200 Icon regulatory.pngIcon rbs.pngIcon coding.png
J23101 + RBS (B0032) + tet R 		September 24
L180 D166 (v) + D109.2 (i) September
L181 D199 (v) + D109.2 (i) September
L182 D187 (v) + D109.2 (i) September
L186 D179 (v) + D205 (i) September
L190 ("L4") D199 (v) + D (i) September
L195 ("L9") D187 (v) + D194 (i) September
L ([http://partsregistry.org/Part:BBa_J23100 J23100]-[http://partsregistry.org/Part:BBa_B0034 B0034 ]-[http://partsregistry.org/Part:BBa_C0079 C0079]-[http://partsregistry.org/Part:BBa_B0015 B0015]) [http://partsregistry.org/Part:J61002 J61002] S169 MP169 Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png
strong constitutive promoter-RBS-LasR-term 		August 21
L ([http://partsregistry.org/Part:BBa_J23101 J23101]-[http://partsregistry.org/Part:BBa_B0034 B0034 ]-[http://partsregistry.org/Part:BBa_C0079 C0079]-[http://partsregistry.org/Part:BBa_B0015 B0015]) [http://partsregistry.org/Part:J61002 J61002] S170 MP170 Icon regulatory.pngIcon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.pngstrong constitutive promoter-RBS-LasR-term August 21
L pCR4Blunt-TOPO S178 MP175 Icon regulatory.pngIcon rbs.png
pFlhB-RBS (WT) 		September 04
L pCR4Blunt-TOPO S MP184 Icon regulatory.pngIcon rbs.png
pFlgB-RBS (WT) 		September 04
L [http://partsregistry.org/Part:J61002 J61002] S201 MP201 Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlgA(RBS WT)-EnvZ* 		September 24
L [http://partsregistry.org/Part:pSB3K3 pSB3K3] S208 MP208 Icon regulatory.pngIcon rbs.pngIcon coding.png
pFlgA(RBS WT)-EnvZ* 		without pFlgA indeed October 04
L [http://partsregistry.org/Part:J61002 J61002] S209 MP209 Icon regulatory.pngIcon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
pFlgA(RBS WT)-EnvZ*-mRFP tripart LVA+ 		without pFlgA indeed October 04
L [http://partsregistry.org/Part:BBa_J61002 J61002] S213 MP213 pFlhB(RBS WT)-mRFP
Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFlhB(RBS WT)-mRFP 		October 10
L [http://partsregistry.org/Part:BBa_J61002 J61002] S214 MP214 Icon regulatory.pngIcon rbs.pngIcon reporter.png
pFliL(RBS WT)-mRFP 		October 10
RBS WT-FlhDC pCR4Blunt-TOPO x MP179 Part icon rbs.pngIcon coding.png
RBS(FlhDC) - FlhDC 		with conserved Pst1 site September 04

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products 2008
Number Name Plamsid Biobricks Description Products in use/comments Conc. ug/mL Date
MP200 J23101 + RBS (B0032) + tet R [http://partsregistry.org/Part:J61002 J61002] Icon regulatory.pngIcon rbs.pngIcon coding.png J23101 + RBS (B0032) + tet R September 24
MP201 pFlgA-EnvZ* [http://partsregistry.org/Part:J61002 J61002] pFlgA-EnvZ*
Icon regulatory.pngIcon rbs.pngIcon coding.png 		pFlgA(RBS WT)-EnvZ* September 24
MP207 pFliA [http://partsregistry.org/Part:BBa_J61002 J61002] pFliA
Icon regulatory.png 		pFliA October 04
MP208 pFlgA-EnvZ* [http://partsregistry.org/Part:pSB3K3 pSB3K3] pFlgA-EnvZ*
Icon regulatory.pngIcon rbs.pngIcon coding.png 		pFlgA(RBS WT)-EnvZ* without pFlgA indeed October 04
MP209 pFlgA-EnvZ*-[http://partsregistry.org/Part:BBa_I732078 I732078] [http://partsregistry.org/Part:J61002 J61002] pFlgA-EnvZ*-mRFP tripart
Icon regulatory.pngIcon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		pFlgA(RBS WT)-EnvZ*-mRFP tripart without pFlgA indeed October 04
MP210 pFliL [http://partsregistry.org/Part:pSB3K3 pSB3K3] pFliL
Icon regulatory.pngIcon rbs.png 		pFliL(RBS WT) October 10
MP211 pFlgA [http://partsregistry.org/Part:pSB3K3 pSB3K3] pFlgA
Icon regulatory.pngIcon rbs.png 		pFlgA(RBS WT) October 10
MP212 pFlhB [http://partsregistry.org/Part:pSB3K3 pSB3K3] pFlhB
Icon regulatory.pngIcon rbs.png 		pFlhB(RBS WT) October 10
MP213 pFlhB [http://partsregistry.org/Part:BBa_J61002 J61002] pFlhB(RBS WT)-mRFP
Icon regulatory.pngIcon rbs.pngIcon reporter.png 		pFlhB(RBS WT)-mRFP October 10
MP214 pFliL [http://partsregistry.org/Part:BBa_J61002 J61002] pFliL(RBS WT)-mRFP
Icon regulatory.pngIcon rbs.pngIcon reporter.png 		pFliL(RBS WT)-mRFP October 10
MP215 [http://partsregistry.org/Part:pSB4T5 pSB4T5] Icon plasmid.png pSB4T5 (low copy plasmid with pSC101 ORI / tetracycline resistance) October 14
MP216 [http://partsregistry.org/Part:BBa_E0434 E0434] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png YFP tripart +AAV October 15

Digestion Products

(Legend: Vector, Insert) Ø

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix (bp) Size BB (bp) Description Product in use []ng/µL Date
D100.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26
D100.2
D101 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2076 15 10 July 29
D102.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 26
August 15
D102.2
D103.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 27 July 26
D103.2 35
D104.1 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26
D104.2
D105.1 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 26
D105.2
D106.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 8 July 26
D106.2
D107 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2750 14 August 23 5
12 		July 26
July 23
D108.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 29
D108.2
D109 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 708 July 26 10 July 26
D110 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2768 14 August 23 20
8
19 		July 26
August 23
August 28
D111.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 26
D111.2
D112 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2079 703 8 September 5
D113 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 779 20 July 26
D114 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 4003 778 20 July 26
D115.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29
D115.2
D116.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29
D116.1
D117 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI SpeI FI 3166 746 5 July 29
D118 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 3167 745 20 July 26
D119 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 743 10 July 26
D120 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 742 10 July 26
D121 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 704 10 July 26
D122 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 703 10 July 26
D123.1 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 26 July 26
D123.2 23
D124.1 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26
D124.2
D125.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 26
10 		July 26
August 23
August 28
D125.2 24
3687
D126.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26
D126.2
D127.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26
D127.2
D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29
D128.1
D129.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29
D129.2
D130.1 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26
D130.2
D130.3
D131.1 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 8 July 26
D131.2
D131.3
D132 pFlgA - EcoRI SpeI FI - 250 5 August 6
D133 pFlgB - EcoRI SpeI FI - 250 5 August 6
D134 pFlhB - EcoRI SpeI FI - 249 5 August 6
D135 pFlhDC - EcoRI SpeI FI - problem August 6
D136 - [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI FV 2925 - August 6
August 16
D137 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FV 2727 - August 6
D138 E0240 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 899 - August 6
D139 flhDC - EcoRI PstI FI - 972 August 6
D140 E040 rbs+ - PstI PstI I - August 12
D141 flhD RBS - - EcoRI SpeI FI - August 12
D142 flhC RBS - - EcoRI SpeI FI - August 12
D143 flhDC + promoter - EcoRI SpeI FI - August 12
D144 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FI - August 12
D145 pSB1A2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - August 12
August 15
D146 MP148 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 2057 1609 rbs TetR - GFP tripart
D110 (BV) - D131 (BI)
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png 		August 15
D147 MP153 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 2056 799 Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)
Part icon rbs.pngIcon coding.png 		August 15
D149 pfliL - EcoRI SpeI FI - August 14
August 16
D150 pfliL - XbaI PstI BI - August 14
August 16
D152 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI - August 14
D153 PCR 138 - gène flhDC - EcoRI SpeI FI - August 15
D154 PCR 140 - gène flhDC - EcoRI SpeI FI - August 15
D155 PCR 139 - pflhDC (URI) - EcoRI SpeI FI - August 15
D158 MP 155.1 - OmpR* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15
D159 MP 156.1 - EnvZ* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15
D161 MP 104 - pTet [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV - 39 August 16
D162 MP 114 - TetR [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - 11 August 16
D163 MP 143 - gfp generator [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 23 14
11 		August 16
August 23
D164 MP 101 - pJ23101 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV - 3 August 16
D165 MP162.2 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] XbaI PstI BI 2057 798 rbs-lasR - double terminator
D147 (FI) - D125 (FV)
Part icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png 		27 August 18
D166 MP 165.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV - 2942 RBS (B0034)+ YFP+ LVA- term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
59 		August 26
September 3
D179 MP3.4 or MP118 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] SpeI PstI BV B0015 (double terminator B0010-B0012)
Part icon terminator.pngPart icon terminator.png 		August 20
D180 MP101.1 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV promoter J23101
Part icon regulatory.png 		August 20
D181 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV pTet (TetR repressible promoter)
Part icon regulatory.png 		August 20
D182 MP114.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI TetR
Icon coding.png 		August 20
D183 MP119.3 - XbaI PstI BI pBad promoter
Part icon regulatory.png 		August 20
D184 MP143.1 [http://partsregistry.org/Part:BBa_pSB2K3 pSB2K3] EcoRI SpeI FI gfp generator
Part icon reporter.png 		August 18
D185 MP163.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV B0032 RBS
Part icon rbs.png 		August 18
D187 MP168.1 [http://partsregistry.org/Part:BBa_pSB1A3 pSB1A3] EcoRI XbaI FV RBS (B0034)+ mRFP LVA+ term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
43
September 3
D188 PCR 138 - XbaI PstI Insert - 767 OmpR*
Icon coding.png 		August 26
D189 MP 101.2 [http://partsregistry.org/Part:BBa_J61002 J61002] XbaI PstI Vector - 2073 pSB1A2
Icon plasmid.png 		August 26
D191 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2724 tetR
Icon coding.png 		22 August 29
D192 MP144.2 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI XbaI FV 2267 FlgA
Part icon regulatory.png 		26 August 29
D193 MP171 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 961 gfp generator(E0240) - pTet
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png 		9,5 August 29
D194 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 704 RBS(B0032) - tetR
Part icon rbs.pngIcon coding.png 		13 August 29
D195 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 703 RBS(B0032) - tetR
Part icon rbs.pngIcon coding.png 		11 August 29
D196 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2119 pTet
Part icon regulatory.png 		45 September 3
D197 MP157.2 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI 898 gfp generator(E0240)
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		12 September 3
D198 MP164.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2981 RBS (B0034)+ ECFP+ LVA+ term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		36 September 3
D199 MP167.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2942 RBS (B0034)+ ECFP LVA- term (B0015)
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		61 September 3
D200 MP171.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 960 gfp generator(E0240) - pTet
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png 		43 September 3
D201 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2724 tetR
Icon coding.png 		13 August 29
D202 MP101.1 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI nothing 2984 25 September 8
D203 MP101.2 [http://partsregistry.org/Part:BBa_J61002 J61002] BspHI nothing 1870
1008
105 		September 11
D204 MP144 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI September 22
D205 MP101 [http://partsregistry.org/Part:BBa_J61002 J61002] XbaI PstI September 22
D206 MP179 XbaI PstI September 22
D207 MP182.3 XbaI PstI September
D208 MP175 EcoRI SpeI September
D209 MP144 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI September
D210 MP178 EcoRI SpeI September

Strains (glycerol stock)

Strains glycerol stock
Number Name Biobricks Description Verified clone Date



S141 MG1655 Aug 7



S157 [http://partsregistry.org/Part:BBa_E0240 E0240] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png GFP generator August 15
S158 [http://partsregistry.org/Part:pSB3K3 pSB3K3] Icon plasmid.png plasmid August 15



S171 L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pTet - gfp Tripart
D106 (BV) - D131 (BI) 		Same as S138 but in chemically labmade competent MG1655 bacteria. For study of doubling time of population Aug 22


S215 [http://partsregistry.org/Part:pSB4T5 pSB4T5] Icon plasmid.png plasmid October 14
S216 [http://partsregistry.org/Part:BBa_E0434 E0434] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png YFP tripart +AAV October 15
Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Freezer"