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From 2008.igem.org

Contents 1 Last number used 2 Freezer 2.1 PCR products 2.2 Biobricks 2.3 Ligations: Constructions realised 2.4 Plasmids: MiniPrep Products 2.5 Digestion Products 2.6 Strains (glycerol stock) Last number used Miniprep Strain Ligation Digestion PCR 216 216 213 202 154 (last checking on 19th october) Freezer PCR products PCR products Number Name Oligo F Oligo R Template Length Comments Product Left ? [DNA]µg/mL Date PCR124 pFlgA O100 O101 - 261pb yes 0.5 August 7 PCR125 pFlgB O102 O103 - 261pb yes 0.5 August 7 PCR126 pFlhB O108 O109 - 260pb yes 0.5 August 7 PCR127 FlhDC O132 O131 MG1655 no August 8 PCR128 OmpR* O138 O139 Strain OmpR* 700pb yes 150 August 8 PCR129 EnvZ* O126 O127 Strain EnvZ* 1400pb yes 100 August 8 PCR130 E0240 rbs+ O141 O140 MP143 900pb no August 11 PCR131 FlhD rbs- O132 O133 MG1655 350pb no August 11 PCR132 pFlhD rbs- O130 O131 MG1655 600pb no August 11 PCR133 FlhDC + prom O110 O131 MG1655 1300pb no August 11 PCR134 FlhDC + prom O111 O131 MG1655 no August 11 PCR135 pFliL O124 O125 MG1655 yes August 11 PCR136 pFlhDC O110 O113 MG1655 yes August 11 PCR137 pFlhDC O 111 O 113 MG1655 yes August 11 PCR138 FlhDC (RBS -) O 132 O 131 MG 1655 yes August 15 PCR139 pFlhDC (URI) O 110 O 113 PCR 130 yes August 15 PCR140 FlhDC (RBS -) O 132 O 131 PCR 130 yes August 15 PCR141 Gene FlhDC + rbs nat O 134 O 131 MG1655 1000pb yes August 26 PCR142 pFliA + rbs nat O 145 O 144 MG1655 325pb yes August 26 PCR143 pFliA sans rbs O 145 O 146 MG1655 310pb yes August 26 PCR144 pFliA + Gene FliA O 145 O 151 MG1655 1100pb yes August 26 PCR145 PCRFliA1 O 148 O 152 MG1655 150pb yes August 26 PCR146 PCRFliA2 O 153 O 150 MG1655 670pb yes August 26 PCR 147 EnvZ* O 126 O 127 S 120 1412 bp yes 150 August 26 PCR 148 OmpR* O 138 O 139 S 119 789 bp yes 101 August 26 PCR 149 pFlhDC (Soutourina +RBS) O 154 O 113 MG1655 450 bp yes August 26 PCR 151 pFLiA without nat. rbs O 145 O 146 MG1655 310 bp yes 34 August 26 PCR 152 pFliA rbs nat. O 145 O 144 MG1655 325 bp yes 19 August 26 PCR 153 pFliA+Gene FliA non-mutated O 145 O 151 MG1655 1100 bp yes 39 August 26 PCR 154 Gene FliA mutated (EcoRI/PstI) O 148 O 150 MG1655 800 bp 154.1, 154.2, 154.3 yes 50 August 26 Biobricks BioBricks Name/N° Plasmid Strain Miniprep Description comment Date [http://partsregistry.org/Part:BBa_B0034 B0034 ] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S107 MP100 Strongest RBS (Efficiency = 1) July 26 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 J61002 ] S109 MP101 Strongest Constitutive promoter July 22, July 23, July 26 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] S110 MP102 Weak Constitutive promoter July 22, July 26 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S108 MP103 promoter pLas (lasr & PAI regulated) July 23, July 26 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S111 MP104 tetR repressible promoter July 23, July 26 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S112 MP105 B0034(rbs) ->LasI July 26 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S113 MP106 B0034(rbs) -> TetR August 24 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S114 MP107 lasR activator with LVA Tag July 23 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S115 MP108 lasR activator July 22, July 23, July 26 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S116 MP109 YFP July 26 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S117 MP110 GFP July 23, July 26 [http://partsregistry.org/Part:BBa_E1010 E1010 ] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S118 MP111 mRFP July 23, July 26 OmpR M57I S119 MP112 OmpR July 23 EnvZ I281S S120 MP113 EnvZ July 23 [http://partsregistry.org/Part:BBa_C0040 C0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S121 MP114 tetR repressor July 23 [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:pSB1A3 pSB1A3] S122 MP115 plasmid July 23 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:BBa_J61002 J61002] S MP116 Strong constitutive promoter x July 26 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:BBa_J61002 J61002] S MP117 Medium constitutive promoter x July 24, July 26 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S MP118 Double terminator x July 24, July 26 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] S103 MP119 align="center"|AraC pBAD July 24, July 26 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S MP120 Strong RBS (Efficiency = 0,6) x July 26 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S164 MP121 ECFP (RBS+LVA+Double Term) July 24, July 26 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] S106 MP122 gfp tri-part; strong rbs July 26 [http://partsregistry.org/Part:BBa_J61002 J61002] [http://partsregistry.org/Part:BBa_J61002 J61002] S142 MP123 plasmid August 5 Ligations: Constructions realised Constructions Name/N° Plasmid Strain Miniprep Description Digestion comment Date L100 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S146 MP147 rbs TetR - ECFP D110 (BV) - D130 (BI) August 13 L101 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S147 MP148 rbs TetR - GFP tripart D110 (BV) - D131 (BI) DO NOT WORK !!!! August 13 L102 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S123 MP124 Strong rbs - YFP D129 (BV) - D118 (BI) August 1 L103 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S124 MP125 Strong rbs - mRFP D129 (BV) - D122 (BI) August 1 L104 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S125 MP126 Strong rbs - lasR activator D129 (BV) - D114 (BI) August 1 L105 [http://partsregistry.org/Part:J61002 J61002] S126 MP127 Strong promoter - ECFP D123 (BV) - D130 (BI) August 1 L106 [http://partsregistry.org/Part:J61002 J61002] S127 MP128 Strong promoter - gfp Tripart D123 (BV) - D131 (BI) August 1 L107 [http://partsregistry.org/Part:J61002 J61002] S128 MP129 Strong promoter - ECFP D103 (BV) - D130 (BI) August 1 L108 [http://partsregistry.org/Part:J61002 J61002] S129 MP130 Strong promoter - gfp Tripart D103 (BV) - D131 (BI) August 1 L109 August L110 [http://partsregistry.org/Part:J61002 J61002] S130 MP131 Medium promoter - gfp Tripart D124 (BV) - D131 (BI) August 1 L111 [http://partsregistry.org/Part:J61002 J61002] S131 MP132 Weak promoter - ECFP D104 (BV) - D130 (BI) August 1 L112 [http://partsregistry.org/Part:J61002 J61002] S132 MP133 Weak promoter - GFP tripart D104 (BV) - D131 (BI) August 1 L113 August L114 [http://partsregistry.org/Part:pSB2K3 pSB2K3] S148 MP149 AracpBAD - gfp tripart D126 (BV) - D131 (BI) August 13 L115 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S133 MP134 pLas - ECFP D105 (BV) - D130 (BI) August 1 L116 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S134 MP135 pLas - gfp Tripart D105 (BV) - D131 (BI) August 1 L117 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S135 MP136 yfp - Double Terminator D125 (FV) - D117 (FI) August 1 L118 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S136 MP137 rfp - Double Terminator D125 (FV) - D121 (FI) August 1 L119 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S137 MP138 lasR activator + LVA - Double Terminator D125 (FV) - D113 (FI) August 1 L120 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S149 MP150 tetR repressible promoter - ECFP D106 (BV) - D130 (BI) August 13 L121 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S138 MP139 pTet - gfp tripart D106 (BV) - D131 (BI) August 1 L122 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S150 MP151 RBS-lasI - ECFP D107 (BV) - D130 (BI) August 13 L123 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S151 MP152 RBS lasI - gfp tripart D107 (BV) - D131 (BI) August 13 L124 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S139 MP140 Strongest RBS - rfp D102 (BV) - D122 (BI) August 1 L125 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S140 MP141 Strongest RBS - yfp D102 (BV) - D118 (BI) August 1 L126 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S152 MP153 Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI) August 13 L128 [http://partsregistry.org/Part:J61002 J61002] S143 MP144 pFlgA-RFP D132 (FI) - D136 (FV) August 12 L129 [http://partsregistry.org/Part:J61002 J61002] S144 MP145 pFlgB-RFP D133 (FI) - D136 (FV) August 12 L130 [http://partsregistry.org/Part:J61002 J61002] S145 MP146 pFlhB-RFP D134 (FI) - D136 (FV) wrong sequencing August 12 L131 [http://partsregistry.org/Part:J61002 J61002] pFlhDC-RFP D136 (FV)- D135 (FI) wrong sequencing August 8 L132 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S153 MP154 flhDC D142(FV) - D139(FI) August 13 L133 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S154 MP155 OmpR* D142(FV) - D140(FI) WT sequence (August 19) August 13 ; August 19 L134 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S155 MP156 EnvZ* D142(FV) - D141(FI) lacks about 300 bp in EnvZ* (August 19) August 13 August 19 L135 [http://partsregistry.org/Part:pSB3K3 pSB3K3] pFlgA D137 (BV) - D133 (BI) don't work August 9 L136 [http://partsregistry.org/Part:pSB3K3 pSB3K3] pFlgB D137 (BV) - D1343 (BI) don't work August 9 L137 [http://partsregistry.org/Part:pSB3K3 pSB3K3] pFlhB D137 (BV) - D135 (BI) don't work August 9 L138 [http://partsregistry.org/Part:pSB3K3 pSB3K3] S156 MP157 gfp generator (E0240) D137(FV) - D138(FI) August 13 L139 [http://partsregistry.org/Part:pSB3K3 pSB3K3] S159 MP158 E0240 RBS+ D140 (PstI) - D144 (ES) seems to have the plasmid without the insert and one with the insert August 15 L140 [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhD D145 (V) - D141 (I) August 12 L141 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S160 MP159 flhC D142 (ES) - D145 (ES) has not been sequenced August 15 L142 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S161 MP160 flhDC+promotor D143 (ES) - D145 (ES) does not have the insert August 15 L143 [http://partsregistry.org/Part:pSB3K3 pSB3K3] x x pFliL in pSB3K3 D149 - D137 didn't work August 14 L144 [http://partsregistry.org/Part:pSB3K3 pSB3K3] x x pFliL in pSB3K3 D150 - D152 didn't work August 14 L145 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x x g FlhDC in pSB1A2 D1543 - D145 didn't work August 16 L146 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x x g FlhDC in pSB1A2 D154 - D145 didn't work August 16 L147 [http://partsregistry.org/Part:J61002 J61002] x x pFlhDC in J61002 D155 - D136 didn't work August 16 L148 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x x rbs (B0034)- OmpR* D158 - D102 didn't work August 16 L149 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x x rbs (B0034)- EnvZ D159 - D102 didn't work August 16 L150 [http://partsregistry.org/Part:pSB1A2 pSB1A2] x MP161 pLas + rbs-tetR-gfp tripart D105 (BV) - D146 (BI) incorrect clone -> to do again August 19 L151 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] S162 MP162 rbs-lasR + double terminator D147 (FI) - D125 (FV) August 19 L153 [http://partsregistry.org/Part:BBa_J61002 J61002] S169 MP169 pJ23100-rbs-lasER-dble ter D123 (BV) - D165 (BI) August 20 L154 [http://partsregistry.org/Part:BBa_J61002 J61002] S170 MP170 pJ23101-rbs-lasER-dble ter D103 (BV) - D165 (BI) August 20 L155 pJ23101-gfp generator D164(FV) - D163 (FI) August 22 L156 pTet-gfp generator D161 (FV) - D163 (FI) August 22 L157 tetR-rbs (B0015) D125 (BV) - D162 (BI) August 23 L158 x x rbs-tetR-gfp generator D110(BV) - D131 (BI) didn't work August 23 L159 x x rbs-lasI-dbl ter D125 (FV) - D109 (FI) didn't work August L160 [http://partsregistry.org/Part:BBa_J61002 J61002] S174 MP173 pFlgA - YFP Tripart (LVA-) D166 (FV) - D132 (FI) Fluorescence OK August 22 L161 [http://partsregistry.org/Part:BBa_J61002 J61002] S175 MP174 pFlgA - YFP Tripart (LVA+) D167 (FV) - D132 (FI) August 22 L162 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] x x rbs-TetR-gfp generator D110 (BV) - D163 (BI) August 22 L163 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] x x rbs-lasI-gfp generator D107 (BV) - D163 (BI) August 22 L166 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S173 MP172 RBS(B0032) - tetR August 25 L167 [http://partsregistry.org/Part:pSB1A2 pSB1A2] S172 MP171 gfp generator(E0240) - pTet August 25 L174 [http://partsregistry.org/Part:J61002 J61002] S MP202 Strong constitutive promoter-GFP generator J23101-E0240 September 26 L176 D125 (v) + D162 (i) September L179 [http://partsregistry.org/Part:J61002 J61002] S200 MP200 J23101 + RBS (B0032) + tet R September 24 L180 D166 (v) + D109.2 (i) September L181 D199 (v) + D109.2 (i) September L182 D187 (v) + D109.2 (i) September L186 D179 (v) + D205 (i) September L190 ("L4") D199 (v) + D (i) September L195 ("L9") D187 (v) + D194 (i) September L ([http://partsregistry.org/Part:BBa_J23100 J23100]-[http://partsregistry.org/Part:BBa_B0034 B0034 ]-[http://partsregistry.org/Part:BBa_C0079 C0079]-[http://partsregistry.org/Part:BBa_B0015 B0015]) [http://partsregistry.org/Part:J61002 J61002] S169 MP169 strong constitutive promoter-RBS-LasR-term August 21 L ([http://partsregistry.org/Part:BBa_J23101 J23101]-[http://partsregistry.org/Part:BBa_B0034 B0034 ]-[http://partsregistry.org/Part:BBa_C0079 C0079]-[http://partsregistry.org/Part:BBa_B0015 B0015]) [http://partsregistry.org/Part:J61002 J61002] S170 MP170 strong constitutive promoter-RBS-LasR-term August 21 L pCR4Blunt-TOPO S178 MP175 pFlhB-RBS (WT) September 04 L pCR4Blunt-TOPO S179 MP176 pFliA-RBS (WT) pFliA with Pst1 site September 04 L pCR4Blunt-TOPO S180 MP177 pFlhDC-RBS (WT) September 04 L pCR4Blunt-TOPO S181 MP178 pFliL-RBS (WT) September 04 L pCR4Blunt-TOPO S182 MP179 RBS(FlhDC) - FlhDC FlhDC with conserved Pst1 site September 04 L pCR4Blunt-TOPO S179 MP180 FliA mutated at EcoR1 and Pst1 site September 04 L pCR4Blunt-TOPO S184 MP181 FliA3 FliA mutated at EcoR1 and Pst1 site September 04 L pCR4Blunt-TOPO S185 MP182 EnvZ* the good one but not mutated for EcoR1 site September 04 L pCR4Blunt-TOPO S186 MP183 OmpR WT OmpR WT not the mutated September 04 L pCR4Blunt-TOPO S MP184 pFlgB-RBS (WT) September 04 L pCR4Blunt-TOPO S188 MP185 OmpR WT OmpR WT not the mutated September 04 L [http://partsregistry.org/Part:J61002 J61002] S201 MP201 pFlgA(RBS WT)-EnvZ* September 24 L [http://partsregistry.org/Part:J61002 J61002] S207 MP207 pFliA October 04 L [http://partsregistry.org/Part:pSB3K3 pSB3K3] S208 MP208 pFlgA(RBS WT)-EnvZ* without pFlgA indeed October 04 L [http://partsregistry.org/Part:J61002 J61002] S209 MP209 pFlgA(RBS WT)-EnvZ*-mRFP tripart LVA+ without pFlgA indeed October 04 L [http://partsregistry.org/Part:pSB3K3 pSB3K3] S210 MP210 pFliL(RBS WT) October 10 L [http://partsregistry.org/Part:pSB3K3 pSB3K3] S211 MP211 pFlgA(RBS WT) October 10 L [http://partsregistry.org/Part:pSB3K3 pSB3K3] S212 MP212 pFlhB(RBS WT) October 10 L [http://partsregistry.org/Part:BBa_J61002 J61002] S213 MP213 pFlhB(RBS WT)-mRFP pFlhB(RBS WT)-mRFP October 10 L [http://partsregistry.org/Part:BBa_J61002 J61002] S214 MP214 pFliL(RBS WT)-mRFP October 10 Plasmids: MiniPrep Products Plasmids: MiniPrep Products 2008 Number Name Plamsid Biobricks Description Products in use/comments Conc. ug/mL Date MP142 [http://partsregistry.org/Part:pSB3K3 pSB3K3] pSB3K3 (low to medium copy plasmid with p15A ORI / kanamycine resistance) August 8 MP143.1 [http://partsregistry.org/Part:BBa_E0240 E0240] [http://partsregistry.org/Part:pSB1A2 pSB1A2] gfp generator 138 August 8 MP143.2 150 MP163.1 [http://partsregistry.org/Part:BBa_B0032 B0032] [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS 80 August 20 MP163.2 79 MP164 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS+ ECFP+ LVA+ term August 20 MP165 [http://partsregistry.org/Part:BBa_E0430 E0430] [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS+ YFP+ LVA- term August 20 MP166 [http://partsregistry.org/Part:BBa_E0432 E0432] [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS+ YFP LVA+ term BUT be careful , it doesn't work. See registry August 20 MP167 [http://partsregistry.org/Part:BBa_E0420 E0420] [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS+ ECFP LVA- term August 20 MP168 [http://partsregistry.org/Part:BBa_I732078 I732078] [http://partsregistry.org/Part:pSB1A3 pSB1A3] RBS+ mRFP LVA+ term August 20 MP175 pFlhB pCR4Blunt-TOPO pFlhB pFlhB-RBS (WT) September 04 MP176 pFliA pCR4Blunt-TOPO pFliA pFliA-RBS (WT) with conserved internal Pst1 restriction site September 04 MP177 pFlhDC pCR4Blunt-TOPO pFlhDC pFlhDC-RBS (WT) September 04 MP178 pFliL pCR4Blunt-TOPO pFliL pFliL-RBS (WT) September 04 MP179 RBS WT-FlhDC pCR4Blunt-TOPO RBS(FlhDC) - FlhDC RBS(FlhDC) - FlhDC with conserved internal Pst1 restriction site September 04 MP180 FliA2 pCR4Blunt-TOPO FliA FliA mutated at EcoR1 and Pst1 internal site September 04 MP181 FliA3 pCR4Blunt-TOPO FliA3 FliA mutated at EcoR1 and Pst1 internal site September 04 MP182 EnvZ* pCR4Blunt-TOPO EnvZ* EnvZ* the good one but not mutated for the internal EcoR1 restriction site September 04 MP183 OmpR WT pCR4Blunt-TOPO OmpR WT OmpR WT not the mutated September 04 MP184 pFlgB pCR4Blunt-TOPO pFlgB pFlgB-RBS (WT) September 04 MP185 OmpR WT OmpR WT OmpR WT not the mutated September 04 MP200 J23101 + RBS (B0032) + tet R [http://partsregistry.org/Part:J61002 J61002] J23101 + RBS (B0032) + tet R September 24 MP201 pFlgA-EnvZ* [http://partsregistry.org/Part:J61002 J61002] pFlgA-EnvZ* pFlgA(RBS WT)-EnvZ* September 24 MP207 pFliA [http://partsregistry.org/Part:BBa_J61002 J61002] pFliA pFliA October 04 MP208 pFlgA-EnvZ* [http://partsregistry.org/Part:pSB3K3 pSB3K3] pFlgA-EnvZ* pFlgA(RBS WT)-EnvZ* without pFlgA indeed October 04 MP209 pFlgA-EnvZ*-[http://partsregistry.org/Part:BBa_I732078 I732078] [http://partsregistry.org/Part:J61002 J61002] pFlgA-EnvZ*-mRFP tripart pFlgA(RBS WT)-EnvZ*-mRFP tripart without pFlgA indeed October 04 MP210 pFliL [http://partsregistry.org/Part:pSB3K3 pSB3K3] pFliL pFliL(RBS WT) October 10 MP211 pFlgA [http://partsregistry.org/Part:pSB3K3 pSB3K3] pFlgA pFlgA(RBS WT) October 10 MP212 pFlhB [http://partsregistry.org/Part:pSB3K3 pSB3K3] pFlhB pFlhB(RBS WT) October 10 MP213 pFlhB [http://partsregistry.org/Part:BBa_J61002 J61002] pFlhB(RBS WT)-mRFP pFlhB(RBS WT)-mRFP October 10 MP214 pFliL [http://partsregistry.org/Part:BBa_J61002 J61002] pFliL(RBS WT)-mRFP pFliL(RBS WT)-mRFP October 10 MP215 [http://partsregistry.org/Part:pSB4T5 pSB4T5] pSB4T5 (low copy plasmid with pSC101 ORI / tetracycline resistance) October 14 MP216 [http://partsregistry.org/Part:BBa_E0434 E0434] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] YFP tripart +AAV October 15 Digestion Products (Legend: Vector, Insert) Ø Digestion Products Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix (bp) Size BB (bp) Description Product in use []ng/µL Date D100.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26 D100.2 D101 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2076 15 10 July 29 D102.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 26 August 15 D102.2 D103.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 27 July 26 D103.2 35 D104.1 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26 D104.2 D105.1 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 26 D105.2 D106.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 8 July 26 D106.2 D107 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2750 14 August 23 5 12 July 26 July 23 D108.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 29 D108.2 D109 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 708 July 26 10 July 26 D110 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2768 14 August 23 20 8 19 July 26 August 23 August 28 D111.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 26 D111.2 D112 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2079 703 8 September 5 D113 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 779 20 July 26 D114 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 4003 778 20 July 26 D115.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29 D115.2 D116.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29 D116.1 D117 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI SpeI FI 3166 746 5 July 29 D118 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 3167 745 20 July 26 D119 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 743 10 July 26 D120 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 742 10 July 26 D121 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 704 10 July 26 D122 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 703 10 July 26 D123.1 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 26 July 26 D123.2 23 D124.1 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26 D124.2 D125.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 26 10 July 26 August 23 August 28 D125.2 24 3687 D126.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26 D126.2 D127.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26 D127.2 D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29 D128.1 D129.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29 D129.2 D130.1 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26 D130.2 D130.3 D131.1 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 8 July 26 D131.2 D131.3 D132 pFlgA - EcoRI SpeI FI - 250 5 August 6 D133 pFlgB - EcoRI SpeI FI - 250 5 August 6 D134 pFlhB - EcoRI SpeI FI - 249 5 August 6 D135 pFlhDC - EcoRI SpeI FI - problem August 6 D136 - [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI FV 2925 - August 6 August 16 D137 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FV 2727 - August 6 D138 E0240 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 899 - August 6 D139 flhDC - EcoRI PstI FI - 972 August 6 D140 E040 rbs+ - PstI PstI I - August 12 D141 flhD RBS - - EcoRI SpeI FI - August 12 D142 flhC RBS - - EcoRI SpeI FI - August 12 D143 flhDC + promoter - EcoRI SpeI FI - August 12 D144 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FI - August 12 D145 pSB1A2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - August 12 August 15 D146 MP148 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 2057 1609 rbs TetR - GFP tripart D110 (BV) - D131 (BI) August 15 D147 MP153 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 2056 799 Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI) August 15 D149 pfliL - EcoRI SpeI FI - August 14 August 16 D150 pfliL - XbaI PstI BI - August 14 August 16 D152 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI - August 14 D153 PCR 138 - gène flhDC - EcoRI SpeI FI - August 15 D154 PCR 140 - gène flhDC - EcoRI SpeI FI - August 15 D155 PCR 139 - pflhDC (URI) - EcoRI SpeI FI - August 15 D158 MP 155.1 - OmpR* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15 D159 MP 156.1 - EnvZ* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15 D161 MP 104 - pTet [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV - 39 August 16 D162 MP 114 - TetR [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - 11 August 16 D163 MP 143 - gfp generator [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 23 14 11 August 16 August 23 D164 MP 101 - pJ23101 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV - 3 August 16 D165 MP162.2 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] XbaI PstI BI 2057 798 rbs-lasR - double terminator D147 (FI) - D125 (FV) 27 August 18 D166 MP 165.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV - 2942 RBS (B0034)+ YFP+ LVA- term B0015 59 August 26 September 3 D179 MP3.4 or MP118 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] SpeI PstI BV B0015 (double terminator B0010-B0012) August 20 D180 MP101.1 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV promoter J23101 August 20 D181 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV pTet (TetR repressible promoter) August 20 D182 MP114.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI TetR August 20 D183 MP119.3 - XbaI PstI BI pBad promoter August 20 D184 MP143.1 [http://partsregistry.org/Part:BBa_pSB2K3 pSB2K3] EcoRI SpeI FI gfp generator August 18 D185 MP163.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV B0032 RBS August 18 D187 MP168.1 [http://partsregistry.org/Part:BBa_pSB1A3 pSB1A3] EcoRI XbaI FV RBS (B0034)+ mRFP LVA+ term B0015 43 September 3 D188 PCR 138 - XbaI PstI Insert - 767 OmpR* August 26 D189 MP 101.2 [http://partsregistry.org/Part:BBa_J61002 J61002] XbaI PstI Vector - 2073 pSB1A2 August 26 D191 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2724 tetR 22 August 29 D192 MP144.2 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI XbaI FV 2267 FlgA 26 August 29 D193 MP171 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 961 gfp generator(E0240) - pTet 9,5 August 29 D194 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 704 RBS(B0032) - tetR 13 August 29 D195 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 703 RBS(B0032) - tetR 11 August 29 D196 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2119 pTet 45 September 3 D197 MP157.2 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI 898 gfp generator(E0240) 12 September 3 D198 MP164.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2981 RBS (B0034)+ ECFP+ LVA+ term B0015 36 September 3 D199 MP167.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2942 RBS (B0034)+ ECFP LVA- term (B0015) 61 September 3 D200 MP171.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 960 gfp generator(E0240) - pTet 43 September 3 D201 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2724 tetR 13 August 29 D202 MP101.1 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI nothing 2984 25 September 8 D203 MP101.2 [http://partsregistry.org/Part:BBa_J61002 J61002] BspHI nothing 1870 1008 105 September 11 D204 MP144 [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI September 22 D205 MP101 [http://partsregistry.org/Part:BBa_J61002 J61002] XbaI PstI September 22 D206 MP179 XbaI PstI September 22 D207 MP182.3 XbaI PstI September D208 MP175 EcoRI SpeI September D209 MP144 [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI September D210 MP178 EcoRI SpeI September Strains (glycerol stock) Strains glycerol stock Number Name Biobricks Description Verified clone Date S141 MG1655 Aug 7 S157 [http://partsregistry.org/Part:BBa_E0240 E0240] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] GFP generator August 15 S158 [http://partsregistry.org/Part:pSB3K3 pSB3K3] plasmid August 15 S163 [http://partsregistry.org/Part:BBa_B0032 B0032] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] medium rbs (efficiency = 0,3) August 20 S165 [http://partsregistry.org/Part:BBa_E0430 E0430] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] strongest rbs YFP LVA- double terminator August 20 S166 [http://partsregistry.org/Part:BBa_E0432 E0432] in [http://partsregistry.org/Part:pSB1A2 pSB1A2 ] strongest rbs YFP LVA+ double terminator August 20 S167 [http://partsregistry.org/Part:BBa_E0420 E0420] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] strongest rbs ECFP LVA- double terminator August 20 S168 [http://partsregistry.org/Part:BBa_I732078 I732078] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] strongest rbs mRFP LVA+ double terminator August 20 S171 L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] pTet - gfp Tripart D106 (BV) - D131 (BI) Same as S138 but in chemically labmade competent MG1655 bacteria. For study of doubling time of population Aug 22 S183 FiA2 in pCR4Blunt-TOPO FliA2 (mutated FliA at internal EcoR1 and Pst1 restriction site) September 4 S187 pFlgB in pCR4Blunt-TOPO pFlgB September 4 S215 [http://partsregistry.org/Part:pSB4T5 pSB4T5] plasmid October 14 S216 [http://partsregistry.org/Part:BBa_E0434 E0434] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] YFP tripart +AAV October 15

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