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TUDelft/14 August 2008
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Today we started a set of new transformation with various slight differences in order to see if it would help in transformation efficiency. | Today we started a set of new transformation with various slight differences in order to see if it would help in transformation efficiency. | ||
| - | We tried solving the punches from the registry in either: | + | We tried solving the punches from the registry in either: <br> |
| - | - old TE | + | - old TE <br> |
| - | - fresh TE | + | - fresh TE <br> |
- H2O | - H2O | ||
| - | And with these we either | + | And with these we either: <br> |
| - | - added 4 ul to 50 ul competent cells | + | - added 4 ul to 50 ul competent cells <br> |
- added 50 ul competent cells to all the DNA solution including the punch | - added 50 ul competent cells to all the DNA solution including the punch | ||
Revision as of 11:32, 15 August 2008
[http://www.tudelft.nl ]
[http://www.tudelft.nl ]
Today we started a set of new transformation with various slight differences in order to see if it would help in transformation efficiency.
We tried solving the punches from the registry in either:
- old TE
- fresh TE
- H2O
And with these we either:
- added 4 ul to 50 ul competent cells
- added 50 ul competent cells to all the DNA solution including the punch
As positive controls we transformed with pUC18 in H2O or in old TE with a blanc paper punch added, both incubated with the other samples at 42 for 20'.
