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Team:TUDelft/Temperature overview
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We want to create an RNA-based system that is able to have differential gene expression when temperature changes take place (kind of similar to the Berkeley iGEM 2006 - riboregulator lock/key part [http://parts.mit.edu/wiki/index.php/Berkeley2006-RiboregulatorsMain]). | We want to create an RNA-based system that is able to have differential gene expression when temperature changes take place (kind of similar to the Berkeley iGEM 2006 - riboregulator lock/key part [http://parts.mit.edu/wiki/index.php/Berkeley2006-RiboregulatorsMain]). | ||
Revision as of 07:35, 1 September 2008
[http://www.tudelft.nl ]
[http://www.tudelft.nl ]
Bacterial Thermometer
We want to create an RNA-based system that is able to have differential gene expression when temperature changes take place (kind of similar to the Berkeley iGEM 2006 - riboregulator lock/key part [http://parts.mit.edu/wiki/index.php/Berkeley2006-RiboregulatorsMain]).
There are several systems suggested in literature that are based on RNA secondary structure. The idea in general is that if the temperature drops below a certain temperature, the RNA will form stable base-pairs on the Shine-Dalgarno sequence, disabling the ribosome to bind. The base-pairing of this RNA region will block the expression of the protein encoded behind it. In this way gene expression can be regulated on the RNA level by temperature.
