TUDelft/22 August 2008

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(New page: {{Template:TUDelftiGEM2008}} {{Template:TUDelftiGEM2008_menu_home}} <div id="tudelft_content"> =22th August= ==DNA extraction test== Today another gel was run with DNA soaked from the s...)
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Revision as of 10:23, 22 August 2008

22th August

DNA extraction test

Today another gel was run with DNA soaked from the spots. To test the soaking-protocol, various steps of the protocol were performed on the DNA. This time 10 ul of TE was used due to the soaking of the water in the punch, reducing free liquid greatly. Again various conditions were tested, and also the later sent spots from the promoter strength testing DNA were analyzed. To give an idea of DNA concentrations, all spots were nanodropped. These results are in the table below.

Nanodrop of extracted DNA
Sample ng/ul A260/A280
TE (also blanc) 0.53 1.42
10 ul TE + 1002 9A punch, Tr 4.94 0.69
10 ul TE + 1002 9A punch, Tr + spinned 9.69 0.89
10 ul TE + 1002 9A punch, T = 42 11.04 0.75
10 ul TE + 1002 9A punch, T = 42 + Spinned 11.78 1.23
10 ul TE + promoter strength punch, Tr 4.81 1.36
10 ul TE + promoter strength punch, 42 C + spinned 11.69 1.66
10 ul TE + 3 ul pSB1A7 (isolated on 20-08-08) 11.69 1.66

These results have again rather low 260/280, but DNA concentrations that should be visible on a gel. That is running right now.


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