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From 2008.igem.org
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- 19:16, 11 June 2008 (diff | hist) N Alberta NINT/11 June 2008 (New page: lab work (SD): BBa_I732018 (XL1-B) plate had 96 colonies. Set up overnights for 2 colonies from that plate in LB + amp50 culture tubes. Incubated at ...)
- 19:22, 10 June 2008 (diff | hist) N File:Sdeane.JPG (top)
- 19:21, 10 June 2008 (diff | hist) Team:Alberta NINT/Team (→The Team (PLEASE MIND WHAT YOU CLICK ON UNTIL THIS GETS SORTED OUT BETTER))
- 19:20, 10 June 2008 (diff | hist) Team:Alberta NINT/Team (→The Team (PLEASE MIND WHAT YOU CLICK ON UNTIL THIS GETS SORTED OUT BETTER))
- 19:19, 10 June 2008 (diff | hist) File:NINT Shannon Deane.JPG (uploaded a new version of "Image:NINT Shannon Deane.JPG") (top)
- 19:18, 10 June 2008 (diff | hist) Team:Alberta NINT/Team (→The Team (PLEASE MIND WHAT YOU CLICK ON UNTIL THIS GETS SORTED OUT BETTER))
- 19:17, 10 June 2008 (diff | hist) N File:NINT Shannon Deane.JPG
- 19:11, 10 June 2008 (diff | hist) N Alberta NINT/10 June 2008 (New page: lab work (SD): Transformed XL1-B cells with BBa_I732018.)
- 22:25, 9 June 2008 (diff | hist) Alberta NINT/9 June 2008
- 22:25, 9 June 2008 (diff | hist) Alberta NINT/9 June 2008
- 20:25, 9 June 2008 (diff | hist) Team:Alberta NINT/Project
- 20:16, 9 June 2008 (diff | hist) N Alberta NINT/9 June 2008 (New page: lab work (SD): K102008 and K102008 negative control plates again had >100 colonies each. Verified sequencing for K102002. Digested K102002 with Ba...)
- 20:14, 9 June 2008 (diff | hist) Alberta NINT/6 June 2008
- 20:11, 9 June 2008 (diff | hist) Alberta NINT/5 June 2008
- 20:06, 9 June 2008 (diff | hist) Alberta NINT/13 May 2008
- 20:05, 9 June 2008 (diff | hist) Alberta NINT/17 May 2008
- 20:04, 9 June 2008 (diff | hist) Alberta NINT/22 May 2008
- 20:03, 9 June 2008 (diff | hist) Alberta NINT/29 May 2008
- 20:02, 9 June 2008 (diff | hist) Alberta NINT/31 May 2008
- 16:40, 5 June 2008 (diff | hist) N Alberta NINT/4 June 2008 (New page: lab work (SD): Transformed XL1-B cells with plasmids K102008, K102009 and negative controls.)
- 20:59, 3 June 2008 (diff | hist) Alberta NINT/3 June 2008
- 17:20, 3 June 2008 (diff | hist) N Alberta NINT/3 June 2008 (New page: lab work (SD): Ran K102002 and K102004 on 1% agarose gel to purify. Used QIAquick nucleotide removal standard protocol to purify T/A 0.)
- 17:19, 3 June 2008 (diff | hist) Alberta NINT/2 June 2008
- 17:18, 3 June 2008 (diff | hist) Alberta NINT/30 May 2008
- 17:18, 3 June 2008 (diff | hist) Alberta NINT/30 May 2008
- 17:17, 3 June 2008 (diff | hist) Alberta NINT/2 June 2008
- 19:16, 30 May 2008 (diff | hist) Team:Alberta NINT/Notebook/catchall (→General Announcements)
- 19:03, 30 May 2008 (diff | hist) N Alberta NINT/30 May 2008 (New page: lab work (SD): DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep protocol. This DNA was set up for sequencing.)
- 19:01, 30 May 2008 (diff | hist) N Alberta NINT/29 May 2008 (New page: lab work (SD): Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B) (plates from 28/05/09). DNA was run on 2% agarose gel. All 9 samples were identical on th...)
- 18:59, 30 May 2008 (diff | hist) N Alberta NINT/28 May 2008 (New page: lab work (SD): Transformed XL1-B cells with the plasmids created 27/05/08. K102002 had 170 colonies. K102004 had 629 colonies. K102000 negative control had 5 colonies.)
- 18:55, 30 May 2008 (diff | hist) Team:Alberta NINT/Team (→The Team (PLEASE MIND WHAT YOU CLICK ON UNTIL THIS GETS SORTED OUT BETTER))
- 18:55, 30 May 2008 (diff | hist) Team:Alberta NINT/Team (→The Team (PLEASE MIND WHAT YOU CLICK ON UNTIL THIS GETS SORTED OUT BETTER))
- 00:20, 28 May 2008 (diff | hist) Alberta NINT/27 May 2008
- 19:25, 27 May 2008 (diff | hist) Team:Alberta NINT
- 18:14, 27 May 2008 (diff | hist) Team:Alberta NINT
- 18:13, 27 May 2008 (diff | hist) Team:Alberta NINT
- 18:13, 27 May 2008 (diff | hist) Team:Alberta NINT
- 18:12, 27 May 2008 (diff | hist) Team:Alberta NINT
- 18:12, 27 May 2008 (diff | hist) Team:Alberta NINT
- 17:56, 27 May 2008 (diff | hist) N Alberta NINT/27 May 2008 (New page: lab work (SD): DNA digest of K102000 with SpeI + PstI. Incubated at 37 C for 1 hour. Run on 2% agarose gel. ~3 kbp fragment expected.)
- 17:55, 27 May 2008 (diff | hist) N Alberta NINT/23 May 2008 (New page: lab work (SD, JD): Isolated plasmid DNA from LB culture tubes from 22/05/08 using QIAprep standard miniprep protocol. Plasmids were sequenced. K102000.4 becomes K102000. K102001 unsucce...)
- 17:53, 27 May 2008 (diff | hist) N Alberta NINT/22 May 2008 (New page: lab work (SD, JD): Colony PCR of eight colonies selected from 21/05/08 K102000 and K102001 plates. Run on 2% agarose gel. K102000.3, K102000.4, K102000.5, K102000.6, K102001.2, K102001.3...)
- 17:50, 27 May 2008 (diff | hist) N Alberta NINT/21 May 2008 (New page: lab work (SD, JD): Transformation of XLI-B cells with K102000 (R0010 + T/A I) and K102001 (R0011 + T/A I). Results: K102000 - 75 colonies. K102000 negative control - 9 colonies.)
- 17:49, 27 May 2008 (diff | hist) N Alberta NINT/20 May 2008 (New page: lab work (SD, JD): DNA extracted from gel purified fragments from 15/05/08 using QIAquick gel extraction standard protocol. Ligation of R0010 + T/A I and R0011 + T/A I.)
- 17:47, 27 May 2008 (diff | hist) N Alberta NINT/17 May 2008 (New page: lab work (SD, JD): PCR of T/A I oligonucleotide using Term + and Term - primers. DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for insertion int...)
- 17:45, 27 May 2008 (diff | hist) N Alberta NINT/15 May 2008 (New page: lab work (SD): DNA digest of R0011.1 and R0010.1 with SpeI + Pst I and E0433.1 with XbaI + PstI. Run on 2% agarose gel. Fragments were excised from gel and frozen overnight at -20 C.)
- 17:42, 27 May 2008 (diff | hist) N Alberta NINT/14 May 2008 (New page: lab work (SD): Isolated plasmid DNA from culture tubes using QIAprep miniprep standard protocol. Digested DNA with EcoRI + PstI. Incubated at 37 C for 2 hrs. Run on 2% agarose gel. Exp...)
- 17:40, 27 May 2008 (diff | hist) Alberta NINT/13 May 2008
- 17:39, 27 May 2008 (diff | hist) N Alberta NINT/13 May 2008 (New page: lab work (SD): Inoculated LB + amp50 culture tubes with ampicillin-resistant colonies containing BBa_R0010, BBa_R0011, BBa_E0433 (x6 tubes). Incubated at 37 C overnight.)
- 17:37, 27 May 2008 (diff | hist) Team:Alberta NINT/Notebook
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