User contributions
From 2008.igem.org
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- 19:54, 26 October 2008 (diff | hist) N File:6-28 mxh 4.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-28 mxh 3.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-28 mxh 2.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-28 mxh 1.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-27 redigest TA.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-25 digest.jpg
- 19:49, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Transformed S1 via Electroporation)
- 19:48, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Transformed S1 via Electroporation)
- 19:48, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Transformed S1 via Electroporation)
- 19:47, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Protocols)
- 19:47, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Tuesday: June 17, 2008)
- 19:47, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Tuesday: June 17, 2008)
- 19:45, 26 October 2008 (diff | hist) N File:PACYC-map.pdf (top)
- 19:44, 26 October 2008 (diff | hist) N Team:Harvard/Plasmids (New page: == Vectors == *pACYC-Duet vector map *[http://partsregistry.org/wiki/index.php?title=Part:pSB3K3 pSB3K3] ==Parts== *[http://spreadsheets.google.com/ccc?key=pwvx...)
- 19:44, 26 October 2008 (diff | hist) Team:Harvard/Notebook (→Notebooks)
- 19:42, 26 October 2008 (diff | hist) Team:Harvard/Team
- 19:41, 26 October 2008 (diff | hist) Team:Harvard/Team (→Advisors)
- 19:40, 26 October 2008 (diff | hist) Team:Harvard/Team (→Teaching Fellows)
- 19:40, 26 October 2008 (diff | hist) Team:Harvard/Team
- 19:39, 26 October 2008 (diff | hist) Team:Harvard/Team
- 19:35, 26 October 2008 (diff | hist) Team:Harvard/Notebook (→Notebooks)
- 19:35, 26 October 2008 (diff | hist) N Team:Harvard/Recipes (New page: =Recipes= ==Orianna's growth media== * 50 mM K-PIPES '''18.925 g/L''' * 7.5 mM NaOH '''7.5 mL/L''' * 28 mM NH<sub>4</sub>Cl '''1.5 g/L''' * 1.3 mM KCl ...)
- 19:34, 26 October 2008 (diff | hist) N Team:Harvard/PrelimTest (New page: =Week 0= ==OD and GFP expression in ''E. Coli''== June 17, 2008 ===3 hrs in Incubation=== {| {{table}} | align="center" style="background:#eeeeee;"|'''Cell Type''' | align="center" style=...) (top)
- 19:32, 26 October 2008 (diff | hist) N Team:Harvard/MFCProtocols (New page: =Running an MFC Experiment from Start to Finish= This page is intended as a comprehensive guide to completing a microbial fuel cell experiment ==Creating a Testing Environment== Begin ...) (top)
- 19:32, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 19:31, 26 October 2008 (diff | hist) N MFCProtocols (New page: =Running an MFC Experiment from Start to Finish= This page is intended as a comprehensive guide to completing a microbial fuel cell experiment ==Creating a Testing Environment== Begin ...) (top)
- 19:26, 26 October 2008 (diff | hist) N Team:Harvard/GenProtocols (New page: ==QIAprep Spin Miniprep Kit== This protocol is designed for the purification of up to 20 ug high-copy plasmid DNA from 1-5 ml overnight ''E. coli'' culture in LB medium. ===Procedure=== #...)
- 19:26, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 19:24, 26 October 2008 (diff | hist) N GenProtocols (New page: ==QIAprep Spin Miniprep Kit== This protocol is designed for the purification of up to 20 ug high-copy plasmid DNA from 1-5 ml overnight ''E. coli'' culture in LB medium. ===Procedure=== #...) (top)
- 19:22, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week14/Chemical and Light (New page: =Mystery Colony PCR 10/08= Colony PCR of putative fungal colonies using primers BBpfx and mtrB500. Image:10-08_Mystery_PCR_MXHTA.jpg Lane 1: 1 kb ladder Lanes 2-6: P136 plate (500 b...) (top)
- 19:22, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week13/Chemical and Light (New page: =Colony PCR 09/30= Should grow only on carb *1-30: 20, 23, 25 (picked all three) --> P38+130 *31-60: 31-60 (picked 31, 35, 39, 42, 46, 50, 55, 60) --> P39+130 Should grow only on kan *61-...) (top)
- 19:22, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week12/Chemical and Light (New page: =9/22 RE digests= With NEB enzymes, 60 min (buffer 2+BSA): *128#1 XPXmnI *128#5 XSXmnI *128#9 ESXmnI The XmnI is to cut the backbone, which has a similar length to the mtrB constructs. W...)
- 19:21, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week11/Chemical and Light (New page: <b>Summary of the week</b> * Retransformed plasmids containing low expression ompR and HO-pcyA (for light system) w/ Cm resistance * Ligated mtrB after an RBS and before a terminator (2 se...) (top)
- 19:21, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week10/Chemical and Light (New page: =Transformations in Shewie= About 250 ng of DNA added each time. ==First Set 8/22== {| {{table}} | align="center" style="background:#f0f0f0;"|'''Plate''' | align="center" style="backgrou...) (top)
- 19:20, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week9/Chemical and Light (New page: =105, mtrB Ligations/transformations 08/17= Parts to ligated came from 8/17 We used a 2:1 molar ratio of insert to vector for all of the ligations. The ligations were all transformed into...) (top)
- 19:20, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week8/Chemical and Light (New page: =MIT lacZ parts RE digests 08/11= {| {{table}} !rowspan=8|Image:8-11_gel_1_MXHTA.jpg !colspan=2 align="center" style="background:#f0f0f0;"|'''1% agarose''' |- | align="center" style="b...) (top)
- 19:19, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week7/Widgetry (New page: =Goals for this week= =Monday: August 4, 2008= =Tuesday: August 5, 2008= =Wednesday: August 6, 2008= =Thursday: August 7, 2008= =Friday: August 8, 2008=)
- 19:19, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week7/Chemical and Light (New page: =PCR= ==8/4: Cph/EnvZ, P1, P3, mtrB not BB== {| {{table}} !rowspan=9|Image:8-04_gel 1_MXHTA.jpg !colspan=2 align="center" style="background:#f0f0f0;"|'''1% agarose gel visualized usin...) (top)
- 19:19, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week6/Widgetry (New page: =Goals for this week= =Monday: July 28, 2008= =Tuesday: July 29, 2008= =Wednesday: July 30, 2008= =Thursday: July 31, 2008= =Friday: August 1, 2008=)
- 19:18, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week6/Chemical and Light (New page: =Sequencing Parts= =Making Thermoinducible cI System = ==Minipreps from Transformations of P75 + P63 7/28== {| {{table}} | align="center" style="background:#f0f0f0;"|'''Sample ID''' | alig...) (top)
- 19:05, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week5/Widgetry (New page: =Monday: July 21, 2008= *Lab meeting *Cleaned chambers =Tuesday: July 22, 2008= *started mtrB and wt cultures *troubleshoot LabVIEW =Wednesday: July 23, 2008= =Thursday: July 24, 2008= =...)
- 19:03, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week5/Chemical and Light (New page: =Testing Thermoinducible Lac System= ==Restreaked Plates of P92-3 in S1 7/21== Restreaked P93 (1:3), P92a, and P93b--all successfully transformed in S1--to detect YFP at 30 and 40 degrees....) (top)
- 19:03, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week4/Widgetry (New page: =Monday: July 14, 2008= * Built three more fuel cell chambers * Ran three wildtype MFC's 600px '''Conclusion:''' *Perhaps cells have died. *Produced negligible a...) (top)
- 19:03, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week4/Chemical and Light (New page: =PCR/primers= ==HO+pcyA, P3 vector w/o GFP== Primers were designed to PCR out the heme oxygenase and pcyA coding regions from the UT Austin plasmids. Due to the RE sites in these nonBB pla...) (top)
- 19:02, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week3/Widgetry (New page: =Goals for this week= #Make Orianna's growth media #construct more chambers to run parallel tests #set up Keithley for multiple tests #grow mtrA, mtrB,wt, E. coli in liquid cultures #negat...)
- 19:02, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week3/Chemical and Light (New page: =Goals for Week 3= =[http://openwetware.org/images/d/dd/7-7monmtg_slides_%28compatible%29.ppt Summary Powerpoint by Amy]= =The Cloning Strategy= ==Goals:== *<b>Lac Plasmid</b>: Hi/low p...) (top)
- 19:01, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week14 (New page: Chemical and Light<br> Widgetry<br>)
- 19:01, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week13 (New page: Chemical and Light<br> Widgetry<br>)
- 19:01, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week12 (New page: Chemical and Light<br> Widgetry<br>) (top)
- 19:01, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week11 (New page: Chemical and Light<br> Widgetry<br>)
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