TUDelft/14 August 2008

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Today we started a set of new transformation with various slight differences in order to see if it would help in transformation efficiency. We tried solving the punches from the registry in either: - old TE - fresh TE - H2O

And with these we either - added 4 ul to 50 ul competent cells - added 50 ul competent cells to all the DNA solution including the punch

As positive controls we transformed with pUC18 in H2O or in old TE with a blanc paper punch added, both incubated with the other samples at 42 for 20'.


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