Imperial College/11 August 2008

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**5mlx3 from pDR111 ''E.coli''DH5 aplha,
**5mlx3 from pDR111 ''E.coli''DH5 aplha,
**10ml from ''B.subtilis''
**10ml from ''B.subtilis''
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===Cloning===
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Finished the cloning scheme

Revision as of 16:26, 12 August 2008

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11 August 2008

Wetlab Team

B.subtilis

  • Today made up LB broth, SOC broth and PEG 6000.
  • We inoculated the following overnight cultures:
    • 5mlx3 from pDR110 E.coli DH5 aplha,
    • 5mlx3 from pDR111 E.coliDH5 aplha,
    • 10ml from B.subtilis


Cloning

Finished the cloning scheme