Imperial College/12 August 2008

From 2008.igem.org

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= 12 August 2008 =
= 12 August 2008 =

Revision as of 19:12, 15 October 2008

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12 August 2008

B.subtilis

  • Mini prep of 5ml E.coli cultures containing the pDR110/pDR111
  • Competent B.subtilis prepared

PCR cloning

  • Designed Primers for PCR cloning of XylR gene and EpsE integration sequences from the B.subtilis chromosome
  • Designed Primers for PCR cloning of AmyE integration sequences and Spectinomycin resistance from plasmid pDR111

DRY lab

  • wrote a code on matlab to obtain velocity, direction, run time and tumble time from the positions of the cell at each frame
  • Did analysis on exponential distribution, plotting of Posterior of parameter lambda in response to obtained synthetic data samples generated.
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