12 August 2008
B.subtilis
- Mini prep of 5ml E.coli cultures containing the pDR110/pDR111
- Competent B.subtilis prepared
PCR Cloning
- Designed Primers for PCR cloning of XylR gene and EpsE integration sequences from the B.subtilis chromosome
- Designed Primers for PCR cloning of AmyE integration sequences and Spectinomycin resistance from plasmid pDR111
Dry Lab
- wrote a code on matlab to obtain velocity, direction, run time and tumble time from the positions of the cell at each frame
- Did analysis on exponential distribution, plotting of Posterior of parameter lambda in response to obtained synthetic data samples generated.
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