14th August 2008

Wet Lab

Parts C0012 (LacI), J31005 (Chloraphemicol acetyltransferase), B0015 (Double terminator), J04630 (GFP and Double Terminator), I13401 (mRFP and Double Terminator) taken from the registry and transformed by electroporation into XL1-Blue E.coli along with XL1-Blue controls and grown on Kanamycin and Ampicillin plates

Dry Lab

• Completed A More Complex Example of Bayesian Parameter Estimation of Tutorial 2.
• Sourced for better tracking algorithm, found SpotTracker which is more accurate than ParticleTracker.
• Went for microscope training, obtained 2 x videos on B.Subtilis motility, stored on server with James.
• Discussion with Dr. Suhail ( from Structural Bioinformatics Group,Imperial College London) on the computing requirements to analyse the cells motility. We will be allocated a linux 64-bit workstation, that we will be able to access remotely via SSH.