Parts taken from the registry and transformed by electroporation into XL1-Blue E.coli along with XL1-Blue controls and grown on Kanamycin and Ampicillin plates. Parts taken:
C0012 (LacI)
J31005 (Chloraphemicol acetyltransferase)
B0015 (Double terminator)
J04630 (GFP and Double Terminator)
I13401 (mRFP and Double Terminator)
Dry Lab
Completed A More Complex Example of Bayesian Parameter Estimation of Tutorial 2.
Sourced for better tracking algorithm, found SpotTracker which is more accurate than ParticleTracker.
Went for microscope training, obtained 2 x videos on B.Subtilis motility, stored on server with James.
Discussion with Dr. Suhail ( from Structural Bioinformatics Group,Imperial College London) on the computing requirements to analyse the cells motility. We will be allocated a linux 64-bit workstation, that we will be able to access remotely via SSH.
Microscope
Chris, Clinton, James and Prudence received microscope training on Widefield 1, recording two 60 second videos of B.subtilis swimming for analysis
Determined that best results would be obtained by bringing a B.subtilis overnight culture to the microscope facility and diluting 100 fold