Transform E.coli Xl1-blue with C0012 (LacI) from the registry again
Tuesday
Check LacI transformants
Miniprep and digest 0.1.01 (Double Terminator) and 0.1.02 (Chloraphenicol Reisitance).
Ligate together to form construct 0.1.14 and transform into Xl1-Blue E.coli
Wednesday
Check E.coli transformed with construct 0.1.14 for growth and by PCR (if growth was succesful)
If primers have arrived, PCR clone LacI, XylR, Spectinomycin, EpsE modified integration sequnce and AmyE integration sequence into Biobricks
Thursday
If grown, mini/midi-prep a few colonies from each plate and digest a sample with XbaI and SpeI to determine which plasmids have the insert in the correct orientation.
If primers arrive today PCR cloning should be carried out
Friday
If primers arrived Thursday check plates for growth and carry out minipreps
PCR check the minipreps that could be digested by XbaI and SpeI
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