Imperial College/1 September 2008

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1st September 2008

Wet Lab

Attempts to count colonies using the illuminator failed. We have decided to count colonies manually instead.

We carried out a PCR experiment in an attempt to clone genes (such as EpsE, AmyE integration sites etc.) from vectors. However, due to problems with the image viewer, we did not manage to take an image of the gel at the end of the day.

Cloning

  • First GeneArt sequences arrived! Constructs 7, 8 9 and 11 (SacB-EAK 16, LipA-EAK16, P43-gsiB and Pveg-gsiB) transformed into XL1-Blue E.coli
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