"
Imperial College/27 August 2008
From 2008.igem.org
(Difference between revisions)
| (3 intermediate revisions not shown) | |||
| Line 5: | Line 5: | ||
|{{#calendar: title=Imperial_College |year=2008 | month=08}} | |{{#calendar: title=Imperial_College |year=2008 | month=08}} | ||
|{{#calendar: title=Imperial_College |year=2008 | month=09}} | |{{#calendar: title=Imperial_College |year=2008 | month=09}} | ||
| - | | rowspan="2" bgcolor=# | + | | rowspan="2" bgcolor=#ffffff width="100%" | |
|} | |} | ||
= 27 August 2008 = | = 27 August 2008 = | ||
==Wetlab== | ==Wetlab== | ||
| - | *Today a Midi prep of | + | *Today a Midi prep of CAT and the ''B.subtilis'' vectors pDR110 and pDR111 were performed. Validation of the DNA was carried out by running a gel electrophoresis of the DNA. |
| + | [[Image:27-8.PNG|center|frame| Lanes : M = Marker, 1 = CAT Midi 1 Digested, 2 =CAT Midi 2 Digested, 3 = pDR110 Midi 1 Undigested, 4 = pDR110 Midi 2 Undigested, 5 = pDR111 Midi 1 Undigested, 6 = pDR111 Midi 2 Undigested]] | ||
| + | |||
| + | * Both the plasmids and the CAT insert appear to be the correct size and contamination appears to be fairly negligible | ||
==Dry Lab== | ==Dry Lab== | ||
| Line 15: | Line 18: | ||
*Obtained a second better quality microscope video of B. Subtilis swimming (better contrast, better focus...) | *Obtained a second better quality microscope video of B. Subtilis swimming (better contrast, better focus...) | ||
*Failed to track the cells using Spot Tracker plugin in ImageJ. More investigation needed. | *Failed to track the cells using Spot Tracker plugin in ImageJ. More investigation needed. | ||
| + | <br> | ||
| + | {{Imperial/EndPage|Notebook|Notebook}} | ||
Latest revision as of 20:40, 28 October 2008
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
27 August 2008Wetlab
 Lanes : M = Marker, 1 = CAT Midi 1 Digested, 2 =CAT Midi 2 Digested, 3 = pDR110 Midi 1 Undigested, 4 = pDR110 Midi 2 Undigested, 5 = pDR111 Midi 1 Undigested, 6 = pDR111 Midi 2 Undigested
Dry Lab
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
