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Imperial College/27 August 2008
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Biofabricator Subtilis
Project Specifications
Why B. subtilis?
B. subtilis: Benefits vs Challenges
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27 August 2008
Wetlab
Today a Midi prep of CAT and the
B.subtilis
vectors pDR110 and pDR111 were performed. Validation of the DNA was carried out by running a gel electrophoresis of the DNA.
Lanes : M = Marker, 1 = CAT Midi 1 Digested, 2 =CAT Midi 2 Digested, 3 = pDR110 Midi 1 Undigested, 4 = pDR110 Midi 2 Undigested, 5 = pDR111 Midi 1 Undigested, 6 = pDR111 Midi 2 Undigested
Both the plasmids and the CAT insert appear to be the correct size and contamination appears to be fairly negligible
Dry Lab
Obtained a second better quality microscope video of B. Subtilis swimming (better contrast, better focus...)
Failed to track the cells using Spot Tracker plugin in ImageJ. More investigation needed.