Performed midi preps for Terminator, GFP-T, RFP-T and AK3, all had high concentrations and good yields
Double digests with Spe and EcoRI were performed on GFP-T and RFP-T and a 1% agarose gel run. The AK3 and terminator constructs will be digested and run a gel tomorrow morning.
Optimised vector genomics and single colony PCR protocols was also performed today. The results of these will be written out in more detail tomorrows notebook.