Team:TUDelft/Color modeling
From 2008.igem.org
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The biosynthetic model is bulit in '''CellDesigner™'''. There are 14 subtrates and 13 enzymes in total. The first subtrate is Acetyl-CoA which is provided to the pathway in a constant level. The last three subtrates are the color products and are consuming so we have degradation links for them. Lycopene, B-carotene and Zeaxanthin give red, orange and yellow colors respectively. | The biosynthetic model is bulit in '''CellDesigner™'''. There are 14 subtrates and 13 enzymes in total. The first subtrate is Acetyl-CoA which is provided to the pathway in a constant level. The last three subtrates are the color products and are consuming so we have degradation links for them. Lycopene, B-carotene and Zeaxanthin give red, orange and yellow colors respectively. | ||
For the 14 reactions of enzyme-subtrate the Michaelis-Menten kinetics is applied and we have the mass action kinetics for the three degradations. | For the 14 reactions of enzyme-subtrate the Michaelis-Menten kinetics is applied and we have the mass action kinetics for the three degradations. | ||
- | According to the | + | According to the kinetic laws there are 14 differential equations for enzyme-subtrate reactions and three for degradations which could be constructed as: |
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Revision as of 14:01, 21 August 2008
In the output we use [http://www.lbl.gov/Science-Articles/Archive/assets/images/2004/Mar-24/Engineering_terpenoids.pdf mevalonate] and [http://parts.mit.edu/igem07/index.php/Image:Zeaxanthin.jpg GPP] pathways together to produce red, orange and yellow colors. The biosynthetic model is bulit in CellDesigner™. There are 14 subtrates and 13 enzymes in total. The first subtrate is Acetyl-CoA which is provided to the pathway in a constant level. The last three subtrates are the color products and are consuming so we have degradation links for them. Lycopene, B-carotene and Zeaxanthin give red, orange and yellow colors respectively. For the 14 reactions of enzyme-subtrate the Michaelis-Menten kinetics is applied and we have the mass action kinetics for the three degradations. According to the kinetic laws there are 14 differential equations for enzyme-subtrate reactions and three for degradations which could be constructed as: