User contributions
From 2008.igem.org
- 04:06, 30 October 2008 (diff | hist) Team:Brown/Project/Analysis (top)
- 04:06, 30 October 2008 (diff | hist) Team:Brown/Project/Analysis
- 04:06, 30 October 2008 (diff | hist) Team:Brown/Project/Analysis (→Bacterial Resistance Testing)
- 03:58, 30 October 2008 (diff | hist) Team:Brown/Project/Analysis (→NaCl Resistance Testing)
- 03:57, 30 October 2008 (diff | hist) Team:Brown/Project/Analysis (→Conductivity Testing)
- 03:55, 30 October 2008 (diff | hist) Team:Brown/Project/Analysis (→Conductivity Testing)
- 03:55, 30 October 2008 (diff | hist) Team:Brown/Project/Analysis
- 03:26, 30 October 2008 (diff | hist) Team:Brown/Project/Analysis
- 03:23, 30 October 2008 (diff | hist) Team:Brown/Project/Analysis
- 03:20, 30 October 2008 (diff | hist) Team:Brown/Project/Analysis
- 03:16, 30 October 2008 (diff | hist) Team:Brown/Project/Analysis (→Optical Density)
- 03:13, 30 October 2008 (diff | hist) Team:Brown/Project/Testing (→Cell Lysis Testing) (top)
- 03:13, 30 October 2008 (diff | hist) Team:Brown/Project/Testing
- 03:02, 30 October 2008 (diff | hist) Team:Brown/Project/Testing
- 02:52, 30 October 2008 (diff | hist) Team:Brown/Project/Analysis
- 02:08, 30 October 2008 (diff | hist) Team:Brown/Project/Analysis
- 23:11, 29 October 2008 (diff | hist) Team:Brown/Project/Analysis
- 23:10, 29 October 2008 (diff | hist) Team:Brown/Project/Analysis
- 21:18, 29 October 2008 (diff | hist) Team:Brown/Project/Analysis (→NaCl Testing)
- 20:45, 29 October 2008 (diff | hist) Team:Brown/Project/Background (top)
- 20:44, 29 October 2008 (diff | hist) Team:Brown/Project/Background
- 20:44, 29 October 2008 (diff | hist) Team:Brown/Project/Background (→The Facts)
- 20:44, 29 October 2008 (diff | hist) Team:Brown/Project/Background (→The Facts)
- 20:43, 29 October 2008 (diff | hist) Team:Brown/Project/Background (→The Facts)
- 20:43, 29 October 2008 (diff | hist) Team:Brown/Project/Background (→The Facts)
- 20:42, 29 October 2008 (diff | hist) Team:Brown/Project/Background (→The Facts)
- 19:57, 29 October 2008 (diff | hist) Team:Brown/Project/Background (→The Facts)
- 19:57, 29 October 2008 (diff | hist) Team:Brown/Project/Background (→The Facts)
- 19:54, 29 October 2008 (diff | hist) Team:Brown/Project/Analysis (→NaCl Testing)
- 19:51, 29 October 2008 (diff | hist) Team:Brown/Project/Analysis (→Resistance Testing)
- 19:06, 29 October 2008 (diff | hist) Brown: Team Resistance/8 July 2008 (top)
- 19:06, 29 October 2008 (diff | hist) Brown: Team Resistance/8 July 2008
- 19:04, 29 October 2008 (diff | hist) Brown: Team Resistance/8 July 2008
- 19:03, 29 October 2008 (diff | hist) Brown: Team Resistance/8 July 2008
- 19:01, 29 October 2008 (diff | hist) N Brown: Team Resistance/8 July 2008 (New page: {{BNBK}} == Cultures from last night taken out 9am Made 1/100 dilutions of the pVJ4 and pRG1 plasmids and incubated from 9:15am to 11:15am (until they reached mod-log phase) Went to Pa...)
- 19:00, 29 October 2008 (diff | hist) N Brown: Team Resistance/11 July 2008 (New page: {{BNBK}} ==11 July 2008== Ran restriction digest of both pDKL02 (IPTG-induced promoter) and pVJ4 at the EcoRI and EcoRV sites (should cut halfway through the cassette) *Mini-prepped the...) (top)
- 18:58, 29 October 2008 (diff | hist) Brown: Team Resistance/14 July 2008 (→Meeting with Professor Jerry Daniels) (top)
- 18:58, 29 October 2008 (diff | hist) Brown: Team Resistance/14 July 2008 (→Meeting with Professor Jerry Daniels)
- 18:56, 29 October 2008 (diff | hist) Brown: Team Resistance/14 July 2008
- 18:52, 29 October 2008 (diff | hist) Brown: Team Resistance/14 July 2008 (→14 July 2008)
- 18:51, 29 October 2008 (diff | hist) Brown: Team Resistance/14 July 2008 (→14 July 2008)
- 18:49, 29 October 2008 (diff | hist) Brown: Team Resistance/14 July 2008 (→14 July 2008)
- 18:48, 29 October 2008 (diff | hist) N Brown: Team Resistance/14 July 2008 (New page: {{BNBK}} ==14 July 2008== Back to lab after meeting → worked with Labview and Maex to fix apparatus Dan came to the lab around 6pm to help me with the system *Got voltage output to w...)
- 18:44, 29 October 2008 (diff | hist) N Brown: Team Resistance/15 July 2008 (New page: {{BNBK}} Taking resistance readings of LB with set-up *V(input) saturating @4.99 with the 9.91kΩ *Try with 1MΩ resistor (0.992MΩ)→ still saturating *Try with 1kΩ→ still saturatin...) (top)
- 18:42, 29 October 2008 (diff | hist) N Brown: Team Resistance/16 July 2008 (New page: {{BNBK}} ==16 July 2008== Kate and Neil began the PCR while I was in class Made a 1/100 dilution of pVJ4 Once culture reached OD of 0.018,I began concentrating the culture for testing...) (top)
- 18:41, 29 October 2008 (diff | hist) N Brown: Team Resistance/17 July 2008 (New page: {{BNBK}} ==17 July 2008== Overnight test→ solution became very goopy and stringy DNA was stuck to the electrodes Three problems we need to solve: 1) What type cell solution do we want...) (top)
- 17:22, 29 October 2008 (diff | hist) Team:Brown/Project/Analysis
- 15:46, 29 October 2008 (diff | hist) Team:Brown/Project/Testing
- 15:44, 29 October 2008 (diff | hist) Team:Brown/Project/Testing
- 15:38, 29 October 2008 (diff | hist) Team:Brown/Project/Strategy (→Electrical Reporting) (top)
- 15:24, 29 October 2008 (diff | hist) Team:Brown/Project/Overview (→Other Applications)
- 15:23, 29 October 2008 (diff | hist) Team:Brown/Project/Overview (→Other Applications)
- 15:22, 29 October 2008 (diff | hist) Team:Brown/Project/Overview (→Other Applications)
- 14:59, 29 October 2008 (diff | hist) Team:Brown/Project/Overview
- 14:53, 29 October 2008 (diff | hist) Team:Brown/Project/Overview (→Application)
- 14:43, 29 October 2008 (diff | hist) Team:Brown/Project/Overview (→Application)
- 14:31, 29 October 2008 (diff | hist) Team:Brown/Project/Overview
- 07:04, 29 October 2008 (diff | hist) Team:Brown/Project/Testing (→Apparatus Design)
- 06:42, 29 October 2008 (diff | hist) Team:Brown/Project/Analysis (→Optical Density)
- 06:32, 29 October 2008 (diff | hist) Team:Brown/Project/Analysis (→Optical Density)
- 06:20, 29 October 2008 (diff | hist) Team:Brown/Project/Analysis (→Optical Density)
- 04:00, 29 October 2008 (diff | hist) Team:Brown/Project/Strategy (→Electrical Reporting)
- 01:00, 29 October 2008 (diff | hist) Team:Brown/Project/Testing (→Testing of the lysis mechanism)
- 00:42, 29 October 2008 (diff | hist) Brown: Team Resistance/3 July 2008 (→3 July 2008) (top)
- 00:39, 29 October 2008 (diff | hist) Brown: Team Resistance/3 July 2008 (→3 July 2008)
- 00:36, 29 October 2008 (diff | hist) Brown: Team Resistance/2 July 2008 (top)
- 00:35, 29 October 2008 (diff | hist) N Brown: Team Resistance/2 July 2008 (New page: {{BNBK}} ==2 July 2008== In order to make our SRRz gene cassette bio-brick compatible, we wanted to PCR our DNA. In the Jain/Mekalanos paper, they inserted a 1.5kb insert into the pBAD1...)
- 00:34, 29 October 2008 (diff | hist) N Brown: Team Resistance/1 July 2008 (New page: {{BNBK}} ==1 July 2008== Run tests with pBAD+arabinose to see if lysis works *culture of pVJ4 plasmid *incubated 15hrs *OD after incubation: tube 1: 0.333 tube 2: 0.239 *Create 1/10...) (top)
- 00:32, 29 October 2008 (diff | hist) N Brown: Team Resistance/30 June 2008 (New page: {{BNBK}} ==30 June 2008== Suggestions from Diana/Adrian *When primers come—add a very small amount of water and THEN dilute *To test whether or not lysis has occurred→ use some kind ...) (top)
- 00:29, 29 October 2008 (diff | hist) N Brown: Team Resistance/27 June 2008 (New page: {{BNBK}} ==27 June 2008== Ran miniprep of our pVJ4 plasmid (plate 28 from Harvard) to isolate DNA for sequencing * Designed and ordered primers to send out with DNA (primer design in Ka...) (top)
- 00:28, 29 October 2008 (diff | hist) N Brown: Team Resistance/26 June 2008 (New page: {{BNBK}} ==26 June 2008== Bacteria Mystery Solved! Used spec in Tatar Lab (5th floor BioMed) and obtained reading of original culture, 1/10 dilution, 1/100 dilution and obtained reading...) (top)
- 00:27, 29 October 2008 (diff | hist) Brown: Team Resistance/24 June 2008 (→24 June 2008) (top)
- 00:24, 29 October 2008 (diff | hist) Team:Brown/Project/Testing
- 00:13, 29 October 2008 (diff | hist) Team:Brown/Project/Overview
- 22:17, 28 October 2008 (diff | hist) Team:Brown/Project/Overview (→Project Overview)
- 07:50, 28 October 2008 (diff | hist) Team:Brown/Project/Overview (→Project Overview)
- 07:49, 28 October 2008 (diff | hist) Team:Brown/Project/Overview (→Project Overview)
- 07:47, 28 October 2008 (diff | hist) Team:Brown/Project/Overview (→Project Overview)
- 07:46, 28 October 2008 (diff | hist) Team:Brown/Project/Overview (→Project Overview)
- 07:46, 28 October 2008 (diff | hist) Team:Brown/Project/Overview (→Project Overview)
- 07:44, 28 October 2008 (diff | hist) Team:Brown/Project/Background
- 07:43, 28 October 2008 (diff | hist) Team:Brown/Project/Background
- 07:41, 28 October 2008 (diff | hist) N File:Detection system 1.jpg (detection system) (top)
- 07:39, 28 October 2008 (diff | hist) Team:Brown/Project/Overview
- 07:32, 28 October 2008 (diff | hist) Team:Brown/Project/Overview
- 06:06, 28 October 2008 (diff | hist) Team:Brown/Project/Overview (→Strategy)
- 05:50, 28 October 2008 (diff | hist) Team:Brown/Project/Overview
- 05:35, 28 October 2008 (diff | hist) Team:Brown (→Image:Brownwelcome.png) (top)
- 05:34, 28 October 2008 (diff | hist) Team:Brown (→Image:Brownwelcome.png)
- 05:27, 28 October 2008 (diff | hist) Team:Brown (→Image:Brownwelcome.png)
- 05:21, 28 October 2008 (diff | hist) Team:Brown (→Image:Brownwelcome.png)
- 21:52, 27 October 2008 (diff | hist) Brown: Team Resistance/26 October 2008 (→Biobricking work) (top)
- 21:51, 27 October 2008 (diff | hist) Brown: Team Resistance/26 October 2008 (→26 October 2008)
- 05:22, 27 October 2008 (diff | hist) Team:Brown/Project/Strategy (→Electrical Reporting)
- 05:13, 27 October 2008 (diff | hist) Team:Brown/Project/Strategy (→Components of auto-lysis device)
- 05:11, 27 October 2008 (diff | hist) Team:Brown/Project/Strategy (→Components of auto-lysis device)
- 05:10, 27 October 2008 (diff | hist) N Team:Brown/Project/Strategy (New page: {{BrownTemp}} ==Components of auto-lysis device== * Inducible promoter: In order to test the efficacy of the “lysis cassette,” our team first took advantage of the well-characteriz...)
- 21:33, 25 October 2008 (diff | hist) Brown: Team Resistance/25 October 2008 (→Conductance/Lysis Re-test)
- 20:45, 25 October 2008 (diff | hist) Brown: Team Resistance/25 October 2008 (→Conductance/Lysis Re-test)
- 19:46, 25 October 2008 (diff | hist) Brown: Team Resistance/25 October 2008 (→Conductance/Lysis Re-test)
- 18:12, 25 October 2008 (diff | hist) Brown: Team Resistance/25 October 2008 (→pVJ4 biobrick)
- 18:04, 25 October 2008 (diff | hist) Brown: Team Resistance/25 October 2008 (→pVJ4 biobrick)
- 17:51, 25 October 2008 (diff | hist) Brown: Team Resistance/25 October 2008 (→25 October 2008)
- 17:51, 25 October 2008 (diff | hist) N Brown: Team Resistance/25 October 2008 (New page: ==25 October 2008== ====Conductance/Lysis Re-test==== Same method as oct 22 but used 50 mL of same culture as control and test For some reason, the conductivity of the control solution ...)
- 18:28, 22 October 2008 (diff | hist) N Team:Brown/Research (New page: {{BrownTemp}})
- 18:26, 22 October 2008 (diff | hist) Team:Brown/Team (→ABOUT THE TEAM)
- 18:24, 22 October 2008 (diff | hist) Brown: Team Resistance/22 October 2008 (→22 October 2008) (top)
- 17:38, 22 October 2008 (diff | hist) Brown: Team Resistance/22 October 2008 (→Conductance test)
- 15:37, 22 October 2008 (diff | hist) N Brown: Team Resistance/22 October 2008 (New page: {{BNBK}} ==22 October 2008== ====Ligation==== pVJ4 lysis cassette and S105 lysis cassette into standard biobrick vector plasmid pSB1AK3 at EcoRI and SpeI *15 ul mixes: *12.37ul diH20 *1...)
- 04:18, 22 October 2008 (diff | hist) N File:Wiki water pic 2.jpg (top)
- 04:17, 22 October 2008 (diff | hist) Team:Brown (→Brown iGEM)
- 04:16, 22 October 2008 (diff | hist) Team:Brown (→Brown iGEM)
- 04:15, 22 October 2008 (diff | hist) Team:Brown (→Brown iGEM)
- 04:12, 22 October 2008 (diff | hist) Team:Brown (→Brown iGEM)
- 04:11, 22 October 2008 (diff | hist) Team:Brown (→Brown iGEM)
- 04:08, 22 October 2008 (diff | hist) Team:Brown (→Brown iGEM)
- 04:06, 22 October 2008 (diff | hist) Team:Brown (→Brown iGEM)
- 04:04, 22 October 2008 (diff | hist) N File:Wiki water 1.jpg (top)
- 04:03, 22 October 2008 (diff | hist) Team:Brown (→Brown iGEM)
- 04:00, 22 October 2008 (diff | hist) Team:Brown/Project/Overview
- 04:00, 22 October 2008 (diff | hist) Team:Brown/Project/Overview
- 03:59, 22 October 2008 (diff | hist) Team:Brown/Project/Overview
- 03:57, 22 October 2008 (diff | hist) N Team:Brown/Project/Overview (New page: ==Brainstorming== ==Strategy== Guidelines for an ideal biosensor: Biological * Uses minimal biological machinery * Direct induction of system by inducer creates a sensitive system *...)
- 03:53, 22 October 2008 (diff | hist) N File:Wiki water pic 1.jpg (top)
- 03:35, 22 October 2008 (diff | hist) Team:Brown (→Brown iGEM)
- 18:23, 21 October 2008 (diff | hist) N Brown: Team Resistance/23 June 2008 (New page: {{BNBK}} ==23 June 2008== Update Meeting with Professor Wessel, Adrian, Diana, Team Limiter *To create apparatus, consider talking to Ed Mullen in Biomed Machine Shop *Contact Jill Krei...) (top)
- 18:19, 21 October 2008 (diff | hist) N Brown: Team Resistance/16 June 2008 (New page: {{BNBK}} Plan for tomorrow: *Glycerol stocks of each plate *Create cell concentrations suspended in water (use microscope) *Begin planning lead promoter (notes 6/13-6/23 in Kate’a nb)) (top)
- 18:14, 21 October 2008 (diff | hist) Brown: Team Resistance/13 June 2008 (→13 June 2008) (top)
- 18:13, 21 October 2008 (diff | hist) Brown: Team Resistance/13 June 2008 (→13 June 2008)
- 18:13, 21 October 2008 (diff | hist) N Brown: Team Resistance/13 June 2008 (New page: {{BNBK}} ==13 June 2008== Attempted experimentation with lysis reagent Ran into problems with apparatus: how to put in shaker and not lose solution from plates? Need apparatus that wil...)
- 18:09, 21 October 2008 (diff | hist) Brown: Team Resistance/12 June 2008 (→Miniprep Prodedure) (top)
- 17:33, 21 October 2008 (diff | hist) N Brown: Team Resistance/8 June 2008 (New page: {{BNBK}} ==June 8, 2008== ====Considerations for gene cassette==== Harvard’s paper: Use of Lambda Phage S and R Gene Products in an Inducible Lysis (Jain and Mekalanos) System for Vib...) (top)
- 17:31, 21 October 2008 (diff | hist) Brown: Team Resistance/7 June 2008 (top)
- 17:30, 21 October 2008 (diff | hist) N Brown: Team Resistance/7 June 2008 (New page: {{BNBK}} ==June 7, 2008== ====Resistance Apparatus Design==== We needed to create an apparatus that could hold multiple plates so that multiple controls and experiments could be run in ...)