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TUDelft/30 September 2008
From 2008.igem.org
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==3A Assembly Thermoparts== | ==3A Assembly Thermoparts== | ||
| - | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115022 K115022], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115036 K115036] (from a frozen pellet [https://2008.igem.org/TUDelft/25_September_2008#September_25th_2008]) and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115027 K115027] were | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115022 K115022], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115036 K115036] (from a frozen pellet [https://2008.igem.org/TUDelft/25_September_2008#September_25th_2008]) and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115027 K115027] were miniprepped. |
| - | After plasmids were obtained, restrictions have been started for K115022, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115024 K115024], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115025 K115025] and K115027 with | + | After plasmids were obtained, restrictions have been started for K115022, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115024 K115024], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115025 K115025] and K115027 with ''Xba''I and ''Pst''I. [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0080 R0080] was cut with ''Eco''RI and ''Spe''I. [http://partsregistry.org/Part:pSB1AT3 pSB1AT3] was cut with ''Eco''RI and ''Pst''I. |
After restrictions were completed, ligations were started o/n for [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115030 K115030], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115032 K115032], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115033 K115033] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115035 K115035]. | After restrictions were completed, ligations were started o/n for [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115030 K115030], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115032 K115032], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115033 K115033] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115035 K115035]. | ||
==Protein Assay== | ==Protein Assay== | ||
| - | We've made a new attempt at a protein assay, because lysis buffer showed to interfere with the measurements in the last assay. To try to compensate we've made a dilution series of BSA in 1000x diluted lysis buffer to make a new calibration line and measure again. | + | We've made a new attempt at a protein assay, because lysis buffer showed to interfere with the measurements in the last assay. To try to compensate we've made a dilution series of BSA in 1000x diluted lysis buffer to make a new calibration line and measure again. Samples of lysed cultures of different temperatures were also diluted 1000x. The new measurements indicated that some samples had "negative" protein content indicating that lysis by Promega lysis buffer is a bad option for our current assay. We will have to look into non-interfering protein assays or optimize lysis by sonication. |
{{Template:TUDelftiGEM2008_sidebar}} | {{Template:TUDelftiGEM2008_sidebar}} | ||
Latest revision as of 13:11, 27 October 2008
[http://www.tudelft.nl ]
[http://www.tudelft.nl ]
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| 25 | 26 | 27 | 28 | 29 | 30 | 31 |
| September | ||||||
| M | T | W | T | F | S | S |
| [http://2008.igem.org/TUDelft/1_September_2008 1] | [http://2008.igem.org/TUDelft/2_September_2008 2] | [http://2008.igem.org/TUDelft/3_September_2008 3] | [http://2008.igem.org/TUDelft/4_September_2008 4] | [http://2008.igem.org/TUDelft/5_September_2008 5] | [http://2008.igem.org/wiki/index.php?title=TUDelft/6_September_2008&action=edit 6] | [http://2008.igem.org/wiki/index.php?title=TUDelft/7_September_2008&action=edit 7] |
| [http://2008.igem.org/TUDelft/8_September_2008 8] | [http://2008.igem.org/TUDelft/9_September_2008 9] | [http://2008.igem.org/TUDelft/10_September_2008 10] | [http://2008.igem.org/TUDelft/11_September_2008 11] | [http://2008.igem.org/TUDelft/12_September_2008 12] | [http://2008.igem.org/wiki/index.php?title=TUDelft/13_September_2008&action=edit 13] | [http://2008.igem.org/wiki/index.php?title=TUDelft/14_September_2008&action=edit 14] |
| [http://2008.igem.org/TUDelft/15_September_2008 15] | [http://2008.igem.org/TUDelft/16_September_2008 16] | [http://2008.igem.org/TUDelft/17_September_2008 17] | [http://2008.igem.org/TUDelft/18_September_2008 18] | [http://2008.igem.org/TUDelft/19_September_2008 19] | [http://2008.igem.org/wiki/index.php?title=TUDelft/20_September_2008&action=edit 20] | [http://2008.igem.org/wiki/index.php?title=TUDelft/21_September_2008&action=edit 21] |
| [http://2008.igem.org/TUDelft/22_September_2008 22] | [http://2008.igem.org/TUDelft/23_September_2008 23] | [http://2008.igem.org/TUDelft/24_September_2008 24] | [http://2008.igem.org/TUDelft/25_September_2008 25] | [http://2008.igem.org/wiki/index.php?title=TUDelft/26_September_2008&action=edit 26] | [http://2008.igem.org/wiki/index.php?title=TUDelft/27_September_2008&action=edit 27] | [http://2008.igem.org/wiki/index.php?title=TUDelft/28_September_2008&action=edit 28] |
| [http://2008.igem.org/TUDelft/29_September_2008 29] | [http://2008.igem.org/TUDelft/30_September_2008 30] | |||||
| October | ||||||
| M | T | W | T | F | S | S |
| [http://2008.igem.org/TUDelft/1_October_2008 1] | [http://2008.igem.org/TUDelft/2_October_2008 2] | [http://2008.igem.org/TUDelft/3_October_2008 3] | [http://2008.igem.org/wiki/index.php?title=TUDelft/4_October_2008&action=edit 4] | [http://2008.igem.org/wiki/index.php?title=TUDelft/5_October_2008&action=edit 5] | ||
| [http://2008.igem.org/TUDelft/6_October_2008 6] | [http://2008.igem.org/TUDelft/7_October_2008 7] | [http://2008.igem.org/TUDelft/8_October_2008 8] | [http://2008.igem.org/TUDelft/9_October_2008 9] | [http://2008.igem.org/TUDelft/10_October_2008 10] | [http://2008.igem.org/wiki/index.php?title=TUDelft/11_October_2008&action=edit 11] | [http://2008.igem.org/wiki/index.php?title=TUDelft/12_October_2008&action=edit 12] |
| [http://2008.igem.org/TUDelft/13_October_2008 13] | [http://2008.igem.org/TUDelft/14_October_2008 14] | [http://2008.igem.org/TUDelft/15_October_2008 15] | [http://2008.igem.org/TUDelft/16_October_2008 16] | [http://2008.igem.org/TUDelft/17_October_2008 17] | [http://2008.igem.org/wiki/index.php?title=TUDelft/18_October_2008&action=edit 18] | [http://2008.igem.org/wiki/index.php?title=TUDelft/19_October_2008&action=edit 19] |
| [http://2008.igem.org/TUDelft/20_October_2008 20] | [http://2008.igem.org/TUDelft/21_October_2008 21] | [http://2008.igem.org/TUDelft/22_October_2008 22] | [http://2008.igem.org/TUDelft/23_October_2008 23] | [http://2008.igem.org/TUDelft/24_October_2008 24] | [http://2008.igem.org/wiki/index.php?title=TUDelft/25_October_2008&action=edit 25] | [http://2008.igem.org/wiki/index.php?title=TUDelft/26_October_2008&action=edit 26] |
| [http://2008.igem.org/wiki/index.php?title=TUDelft/27_October_2008&action=edit 27] | [http://2008.igem.org/wiki/index.php?title=TUDelft/28_October_2008&action=edit 28] | [http://2008.igem.org/wiki/index.php?title=TUDelft/29_October_2008&action=edit 29] | [http://2008.igem.org/wiki/index.php?title=TUDelft/30_October_2008&action=edit 30] | [http://2008.igem.org/wiki/index.php?title=TUDelft/31_October_2008&action=edit 31] | ||
September 30th
3A Assembly Thermoparts
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K115022 K115022], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115036 K115036] (from a frozen pellet [1]) and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115027 K115027] were miniprepped.
After plasmids were obtained, restrictions have been started for K115022, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115024 K115024], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115025 K115025] and K115027 with XbaI and PstI. [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0080 R0080] was cut with EcoRI and SpeI. [http://partsregistry.org/Part:pSB1AT3 pSB1AT3] was cut with EcoRI and PstI.
After restrictions were completed, ligations were started o/n for [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115030 K115030], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115032 K115032], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115033 K115033] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K115035 K115035].
Protein Assay
We've made a new attempt at a protein assay, because lysis buffer showed to interfere with the measurements in the last assay. To try to compensate we've made a dilution series of BSA in 1000x diluted lysis buffer to make a new calibration line and measure again. Samples of lysed cultures of different temperatures were also diluted 1000x. The new measurements indicated that some samples had "negative" protein content indicating that lysis by Promega lysis buffer is a bad option for our current assay. We will have to look into non-interfering protein assays or optimize lysis by sonication.
