"
Imperial College/10 September 2008
From 2008.igem.org
(Difference between revisions)
(→Growth curve) |
(→Growth curve) |
||
| Line 16: | Line 16: | ||
Second trial run to determine the optimal temperatures for PCR was conducted for epsE 5' insertion sequence, epsE 3' insertion sequence and xylR. Our results are shown below: | Second trial run to determine the optimal temperatures for PCR was conducted for epsE 5' insertion sequence, epsE 3' insertion sequence and xylR. Our results are shown below: | ||
| - | ===Growth | + | ===Growth Curve=== |
| - | We performed an | + | We performed an OD<sub>600</sub> against time growth curve for ''B. subtilis''. The results are shown below: |
| + | |||
[[Image:OD600 v Time.PNG| center]] | [[Image:OD600 v Time.PNG| center]] | ||
| + | |||
| + | Please click on the link below for the Excel data file: | ||
[[Media:OD 600edited.xls]] | [[Media:OD 600edited.xls]] | ||
{{Imperial/EndPage}} | {{Imperial/EndPage}} | ||
Revision as of 10:05, 11 September 2008
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10 September 2008Wet LabTransformationPCRSecond trial run to determine the optimal temperatures for PCR was conducted for epsE 5' insertion sequence, epsE 3' insertion sequence and xylR. Our results are shown below: Growth CurveWe performed an OD600 against time growth curve for B. subtilis. The results are shown below:
 Please click on the link below for the Excel data file: Media:OD 600edited.xls
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
