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Imperial College/10 September 2008
From 2008.igem.org
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===Transformation=== | ===Transformation=== | ||
===PCR=== | ===PCR=== | ||
| - | Second trial run to determine the optimal temperatures for PCR was conducted for epsE 5' | + | Second trial run to determine the optimal temperatures for PCR was conducted for epsE 5' integration sequence, epsE 3' integration sequence and xylR. Our results are shown below: |
===Growth Curve=== | ===Growth Curve=== | ||
Revision as of 16:34, 11 September 2008
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10 September 2008Wet LabTransformationPCRSecond trial run to determine the optimal temperatures for PCR was conducted for epsE 5' integration sequence, epsE 3' integration sequence and xylR. Our results are shown below: Growth CurveWe performed an OD600 against time growth curve for B. subtilis. The results are shown below:
 Please click on the link below for the Excel data file:
Dry LabMotilityTook 20 videos, binned x4 to a frame rate of 27.9fps, more than twice the rate at which previous videos were taken. However, video quality is not as good as before, but cells can still be easily distinguished. Growth CurveObtained sigmoidal curve for bacteria growth using MATLAB.
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