Imperial College/12 September 2008

From 2008.igem.org

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{| cellpadding="5" style="background:#66aadd; border: 4px solid #66aadd; color:black" align="center"
{| cellpadding="5" style="background:#66aadd; border: 4px solid #66aadd; color:black" align="center"
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|<font size=4px><font color=white><center>'''OD<sub>600</sub>'''</center>
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|<font size=2px><font color=white><center>'''OD<sub>600</sub>'''</center>
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|<font size=4px><font color=white><center>'''Dilution Factor'''</center>
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|<font size=2px><font color=white><center>'''Dilution Factor'''</center>
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|<font size=4px><font color=white><center>'''Colony Forming Units'''</center>
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|<font size=2px><font color=white><center>'''Colony Forming Units'''</center>
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|-
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|style="background:#FFFFFF"| 0.93
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|style="background:#FFFFFF"| 6
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|style="background:#FFFFFF"|145
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|-
 +
|style="background:#FFFFFF"|0.93
 +
|style="background:#FFFFFF"|7
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|style="background:#FFFFFF"|13
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|-
 +
|style="background:#FFFFFF"|1.5
 +
|style="background:#FFFFFF"|6
 +
|style="background:#FFFFFF"|206
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|-
 +
|style="background:#FFFFFF"|1.5
 +
|style="background:#FFFFFF"|7
 +
|style="background:#FFFFFF"|26
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|-
 +
|style="background:#FFFFFF"|1.98
 +
|style="background:#FFFFFF"|7
 +
|style="background:#FFFFFF"|121
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|-
 +
|style="background:#FFFFFF"|2.5
 +
|style="background:#FFFFFF"|7
 +
|style="background:#FFFFFF"|52
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|-
 +
|style="background:#FFFFFF"|2.5
 +
|style="background:#FFFFFF"|8
 +
|style="background:#FFFFFF"|4
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|-
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|style="background:#FFFFFF"|3
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|style="background:#FFFFFF"|7
 +
|style="background:#FFFFFF"|230
 +
|-
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|style="background:#FFFFFF"|3
 +
|style="background:#FFFFFF"|8
 +
|style="background:#FFFFFF"|25
|}
|}
[[Image:Od600 vs cell number.PNG|500px|thumb|centre]]
[[Image:Od600 vs cell number.PNG|500px|thumb|centre]]
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*From the results it is clear that numerous repeats are needed, in addition the data helps us to narrow down the suitable dilution factors we can use in the dilution plating.

Revision as of 14:48, 15 September 2008

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12 September 2008

Wet Lab

B.subtilis

  • A test protocol for the calibration curve for OD600 vs colony forming units was carried out today. The aim was to define suitable ranges of dilution plating that can be used across a suitable range of OD600 values.
  • The results are summarised on the graph below and the dilution factors used in the table below:
OD600
Dilution Factor
Colony Forming Units
0.93 6 145
0.93 7 13
1.5 6 206
1.5 7 26
1.98 7 121
2.5 7 52
2.5 8 4
3 7 230
3 8 25


Od600 vs cell number.PNG
  • From the results it is clear that numerous repeats are needed, in addition the data helps us to narrow down the suitable dilution factors we can use in the dilution plating.

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