From 2008.igem.org
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- | =15th August 2008= | + | =15 August 2008= |
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- | ==Wet Lab== | + | =='''Wet Lab'''== |
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| ===Cloning=== | | ===Cloning=== |
Revision as of 23:16, 10 September 2008
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15 August 2008
Wet Lab
Cloning
- Plates form yestreday's transformations checked.
- J31005, B0015, J04650 and I13401 transformants grew overnight but no growth was registered for the C0012 transformant nor the controls.
- PCR primers for XylR, LacI and AmyE integration sequences designed and sent for synthesis
B.subtilis
- The transformation protocol 2 (click link here for the protocol) was performed yesterday. The transformed cells have not yet grown on the LB agar plates containing 100ug/ul streptinomycin. We will leave over the weekend to grow further.
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