J31005, B0015, J04650 and I13401 transformants grew overnight but no growth was registered for the C0012 transformant nor the controls.
PCR primers for XylR, LacI and AmyE integration sequences designed and sent for synthesis
B.subtilis
The transformation protocol 2 (click link here for the protocol) was performed yesterday. The transformed cells have not yet grown on the LB agar plates containing 100ug/ul streptinomycin. We will leave over the weekend to grow further.