From 2008.igem.org
(Difference between revisions)
|
|
Line 8: |
Line 8: |
| |} | | |} |
| | | |
- | =18th August 2008= | + | =18 August 2008= |
| | | |
- | ==Wet Lab== | + | =='''Wet Lab'''== |
| | | |
| ===Cloning=== | | ===Cloning=== |
Revision as of 23:16, 10 September 2008
|
18 August 2008
Wet Lab
Cloning
- Terminator, mRFP - Terminator and pSB1AK3 plasmid containing XL1-Blue cells replica plated and overnight cuture prepared
B.subtilis
- The transformation performed on 15th August 2008 was successful yielding between 1-10 transformed colonies. It was noted that the efficiency of transformation increased with increasing concentrations of DNA. We wish to repeat this protocol, this time using a negative control of competent cells transformed with just water. To do this 20ml of overnight culture of B.subtilis was inoculated.
|